@article{Wan Noor Azmi_Hashim_Suhaili_Misbah_Zakaria_2023, title={Identification of Vermamoeba vermiformis and Tetramitus sp. Isolated from the Gills of Oreochromis sp. (Red Hybrid Tilapia)}, volume={30}, url={https://journal.ipb.ac.id/index.php/hayati/article/view/41436}, DOI={10.4308/hjb.30.3.404-412}, abstractNote={<p>This study is the first report of an infestation of amoeba in the gills of asymptomatic <em>Oreochromis</em> sp. from the Manir River, Terengganu, Malaysia. The results confirmed the presence of two species of amoeba, <em>Vermamoeba vermiformis</em> and <em>Tetratmitus</em> sp., based on the 18 ribosomal RNA gene sequences with 99% sequence similarity. Morphological observations using light and scanning electron microscopy supported the findings, demonstrating both amoeba species’ specific features and locomotions. In the trophozoite stage, the mean size of <em>V. vermiformis</em> in the locomotive form was between 25.55 µm in length and 4.17 µm in width. The mean size of <em>Tetramitus</em> sp. was 20.96 µm in length and 8.66 µm in width. The diameter of the cyst for <em>V. vermiformis </em>was 4.67 µm and <em>Tetramitus</em> sp. was 3.37 µm. <em>V. vermiformis</em> was characterized by cylindrical trophozoites and single monopodial morphology. <em>Tetramitus</em> sp. showed a limax-type and various cell shapes. Infestation of the amoeba was also confirmed by histopathological observation in the lamella region with distinct amoeba characteristics compared to the lamellae’s epithelial tissue. These findings revealed the presence of <em>V. vermiformis</em> and <em>Tetramitus</em> sp. infestation in the gills of <em>Oreochromis</em> sp. and its potential pathogenic activity, whilst the symbiosis interaction in <em>Oreochromis</em> sp. is still unidentified.</p&gt;}, number={3}, journal={HAYATI Journal of Biosciences}, author={Wan Noor AzmiWan NorNadhirah and HashimFatimah and Suhaili, Zarizal and Misbah, Suzana and ZakariaNor Hafizah}, year={2023}, month={Jan.}, pages={404-412} }