@article{Al-Mughni_Daryono_2020, title={Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene}, volume={27}, url={https://journal.ipb.ac.id/index.php/hayati/article/view/31501}, DOI={10.4308/hjb.27.2.174}, abstractNote={<p>Melon (<em>Cucumis melo</em> L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. ’Meloni’ is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The ’Meloni’ plant produces females flowers that expressed by <em>CmACS-7</em> gene and it can be expected that melon plants ’Meloni’ included in the primitive cultivars, while ’Meloni’ is a cultivation cultivar. The aim of this research is to study the relation of ’Meloni’ kinship to primitive and modern cultivars. Characterization of the <em>CmACS-7</em> gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on <em>CmACS-7</em> gene expression using MEGA 7.0. Result of this study show that the presence of the <em>CmACS-7</em> gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while ’Hikapel’ separated from other cultivars.</p&gt;}, number={2}, journal={HAYATI Journal of Biosciences}, author={Al-MughniEka Wasi’ and DaryonoBudi Setiadi}, year={2020}, month={Apr.}, pages={174} }