@article{Priyatno_Bakar_Redzuan_Mahadi_Murad_2020, title={Functional Analysis of an Appressorium-Specific Gene from Colletotrichum gloeosporioides}, volume={27}, url={https://journal.ipb.ac.id/index.php/hayati/article/view/31495}, DOI={10.4308/hjb.27.2.107}, abstractNote={<p>A novel gene (<em>CAS2</em>) specifically expressed during appressorium formation was isolated from <em>Colletotrichum gloeosporioides</em> using Differential Display RT-PCR. <em>CAS2</em> comprises 368 deduced amino acid residues and is 50% identical to a hypothetical protein from <em>Chaetomium globosum</em>. ProtFun 2.2 server analysis predicted that Cas2 functions as a transport and binding protein. Based on putative transmembrane domain prediction software (HMMTOP), Cas2 protein is composed of five alpha-helical transmembrane domains with a very short external N-terminus tail and long internal C-terminus. ExPASy ScanProsite analysis showed the presence of <em>integrin beta chain cysteine-rich domain</em>, N-myristoylation site, <em>EGF-like domain</em>, <em>2Fe-2S ferredoxins, iron-sulfur binding region, VWFC domain, fungal hydrophobins signature</em>, membrane lipoprotein lipid attachment site, and <em>Janus-faced atracotoxin (J-ACTX) family signature</em> in <em>CAS2</em> protein. Mutants with deleted <em>CAS2</em> were not significantly different in terms of vegetative growth, conidiation, and appressoria production compared to wild type. However, the Cas2 mutant produced multipolar germination, a feature which distinguishes it from wild type strain. Interestingly, the mutant is non-virulent to mango fruits, indicating that <em>CAS2</em> may encode proteins that function as novel virulence factors in fungal pathogens.</p&gt;}, number={2}, journal={HAYATI Journal of Biosciences}, author={PriyatnoTri Puji and BakarFarah Diba Abu and RedzuanRohaiza Ahmad and MahadiNor Muhammad and MuradAbdul Munir Abdul}, year={2020}, month={Apr.}, pages={107} }