Microscopical Evaluation and TLC Analysis of Pluchea indica (L.) Less: Leaf, Stem, and Root

  • Ni Putu Ermi Hikmawanti Graduate Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Cluster of Health Sciences Building, Depok 16424, Indonesia. Department of Pharmaceutical Biology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, Jakarta 13460, Indonesia. National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Fadlina Chany Saputri Department of Pharmacology-Toxicology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia. National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Arry Yanuar Department of Biomedical Computation-Drug Design, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia. National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Ratih Asmana Ningrum National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia. Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Raya Bogor Street KM.46, Cibinong, Bogor 16911, Indonesia
  • Abdul Mun'im Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Cluster of Health Sciences Building, Depok 16424, Indonesia. National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Hayati Hayati Department of Pharmaceutical Biology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, Jakarta 13460, Indonesia

Abstract

Pluchea indica (L.) Less is traditionally utilized to treat postpartum women in Indonesia. The plant has many pharmacological properties, so that it can be further developed as herbal medicine. In that development process, plant authentication is needed to ensure the quality of raw materials. A simple microscopical and thin-layer chromatography (TLC) analysis might be a way to authenticate the plant, but it has never been reported. So, this study evaluates the microscopical and TLC analysis for P. indica authentication in standardized herbal medicines production. Plant microscopic observation, fluorescence analysis, and polyphenol screening were conducted. n-Hexane, ethyl acetate, and methanol extracts of plant organs were then analyzed by TLC. Here, we reported that in microscopical analysis the simplicia of P. indica contains trichomes and tannin-containing cells. In addition, chlorogenic acid as a marker was present in TLC analysis by ethyl acetate-water-formic acid-acetic acid (8.5:1.5:1:1, v/v). The results of this evaluation might provide additional information in the identification, authentication, and quality control of P. indica as a raw material for herbal medicine.

Downloads

Download data is not yet available.
Published
2023-10-11
How to Cite
HikmawantiN. P. E., SaputriF. C., YanuarA., NingrumR. A., Mun’imA., & HayatiH. (2023). Microscopical Evaluation and TLC Analysis of Pluchea indica (L.) Less: Leaf, Stem, and Root. HAYATI Journal of Biosciences, 31(1), 71-81. https://doi.org/10.4308/hjb.31.1.71-81