Paederia foetida Linn Leaves-Derived Extract Showed Antioxidant and Cytotoxic Properties Against Breast Carcinoma Cell
Abstract
Paederia foetida Linn. is a rich source of active compounds, yet its antioxidant and cytotoxic properties remain poorly studied. This current research aimed to investigate the chemical profiles of a crude extract derived from P. foetida leaves, its fractionated substances, and their antioxidant and cytotoxic properties on MCF-7 cells, a breast carcinoma cell. Six fractionated substances have been separated in thin layer chromatography (TLC) plate. Five fractions showed antioxidant properties, as indicated by the formation of the yellowish band on the TLC plate after spraying with the DPPH reagent. Furthermore, crude extract of P. foetida leaves and its fractions are also characterized by antioxidant properties against 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. F1 fraction exhibited the most potent antioxidant activity with IC50 values of 10.94±2.67 and 50.04±0.48 µg/ml, respectively, toward DPPH and ABTS radicals. Crude extract and all six fractions also varied in their cytotoxic properties against MCF-7 cells, with IC50 values ranging from 410.24±0.30 to 831.57±6.91 µg/ml. F3 fractions showed the strongest cytotoxicity (IC50 value: 410.24±0.30 µg/ml). Methanolic extract and active plant leaf fractions contained flavonoid and phenolic compounds, as measured by aluminum chloride and Folin-Ciocalteu reagent, respectively. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis showed that phenol, flavonoid, alkaloid, limonoid, and steroid were the major compound found in the crude extract and fractions. In conclusion, the present study proved that crude extract of P. foetida leaves and its fractionated substances were potential as antioxidant and cytotoxic agents.
Downloads
Copyright (c) 2023 Jepri Agung Priyanto, Muhammad Eka Prastya, Gian Primahana, Ahmad Randy, Diyah Tri Utami
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
HAYATI J Biosci article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation.
Authors who submit and publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/
licenses/by-nc/4.0/ Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. - Authors can still use their work commercially
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
.png){kind=logo}
Department of Biology
