In Vitro and In Vivo Malondialdehyde Inhibition Activities of Stichopus hermanii and Spirulina platensis

  • Mega Safithri Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia. Division of Marine Biotechnology, Center for Coastal and Marine Resources Studies, Bogor Agricultural University, Bogor, Indonesia
  • Kustiariyah Tarman Department of Aquatic Products Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, Indonesia. Division of Marine Biotechnology, Center for Coastal and Marine Resources Studies, Bogor Agricultural University, Bogor, Indonesia
  • Iriani Setyaningsih Department of Aquatic Products Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, Indonesia. Division of Marine Biotechnology, Center for Coastal and Marine Resources Studies, Bogor Agricultural University, Bogor, Indonesia
  • Yanti Fajarwati Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia
  • Imanniar Yuta Ellana Dittama Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia

Abstract

Previous research showed that Stichopus hermannii and Spirulina platensis had an antioxidant activity. It is indicated by the reduced malondialdehyde (MDA) level in the liver of the diabetic rats. However, the STZ administration did not significantly increase MDA concentration of diabetic rats' blood serum for 14 days. This research aimed to determine in vitro and in vivo MDA inhibition of S. hermanii and S. platensis. The in vitro antioxidant activity test was conducted using the MDA-TBA method, and a positive control used α-tocopherol. For in vivo experiment, diabetic rats (DM) were induced by streptozotocin for 21 days. Twenty-five rats which were divided into five groups: normal rat group (NA), diabetic rat group (DA), diabetic rat group + glibenclamide (DG), diabetic rats + Stichopus hermanii (SH), and diabetic rats + Spirulina platensis (SP). The in vitro results showed that the antioxidant activity of 25 ppm Spirulina platensis had the same MDA inhibitory activity as 200 ppm α-tocopherol, but 200 ppm Stichopus hermanii had lower inhibition than 200 ppm α-tocopherol. The in vivo result showed that Stichopus hermanii treatment was more effective in suppressing blood serum MDA concentration, but Spirulina platensis was more effective in suppressing liver MDA concentration.

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Published
2022-06-28
How to Cite
SafithriM., TarmanK., SetyaningsihI., FajarwatiY., & DittamaI. Y. E. (2022). In Vitro and In Vivo Malondialdehyde Inhibition Activities of Stichopus hermanii and Spirulina platensis. HAYATI Journal of Biosciences, 29(6), 771-781. https://doi.org/10.4308/hjb.29.6.771-781