Nicotiana tabacum var. Virginia Bio Oil-based Pyrolysis Extraction Have Prominence Antimicrobial Potential Compared to Ethanol Heat Reflux Extraction (EHRE)

  • Andri Pramesyanti Pramono Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Basra Ahmad Amru Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Halimah Anggi Rahmani Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Sheila Azelya Fernanda Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Yudhi Nugraha National Research and Innovation Agency Republic of Indonesia, Jakarta, Indonesia
  • Muhammad Yusuf Arya Ramadhan Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia
  • Andre Fahriz Perdana Harahap Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia
  • Ahmad Fauzantoro National Research and Innovation Agency Republic of Indonesia, Jakarta, Indonesia
  • Nasihin Saud Irsyad Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Meiskha Bahar Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Oktania Sandra Puspita Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Fajriati Zulfa Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia
  • Kori Yati 4Faculty of Pharmacy and Science, Universitas Muhammadiyah Prof. DR. HAMKA, Jakarta, Indonesia. 5Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • Mahdi Jufri Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • Misri Gozan Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia

Abstract

Tobacco leaf contains antibacterial secondary metabolite compounds, such as phenol, alkaloids, and essential oils. This study compares the potential antibacterial effects of Indonesian tobacco leaf extracted using the heat reflux method (producing an extract) and pyrolysis method (providing a bio-oil). The tobacco leaf extract was challenged against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. The bio-oil from the pyrolysis method showed inhibitory Kirby Bauer zones higher than those of the extract from heat reflux method, with the maximum results in the pyrolysis method indicating zones of 6.35 mm (S. aureus), 5.90 mm (E. faecalis), 3.97 mm (E. coli), and 5.025 mm (P. aeruginosa). Further study analyzed the effectiveness of the disc and well diffusion antibacterial test methods for measuring the antibacterial effect of bio-oils against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The bio-oil used in the well diffusion test method showed the most significant antibacterial effectiveness. It showed the biggest inhibition zone, with a maximum of 11.65 mm and 8.90 mm for E. coli and P. aeruginosa. Our results showed Nicotiana tabacum var. Virginia Bio Oil from Ponorogo (Indonesia) is a strong potential antimicrobial, especially using well diffusion test.

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Published
2022-04-19
How to Cite
PramonoA. P., AmruB. A., RahmaniH. A., FernandaS. A., NugrahaY., RamadhanM. Y. A., HarahapA. F. P., FauzantoroA., IrsyadN. S., BaharM., PuspitaO. S., ZulfaF., YatiK., JufriM., & GozanM. (2022). Nicotiana tabacum var. Virginia Bio Oil-based Pyrolysis Extraction Have Prominence Antimicrobial Potential Compared to Ethanol Heat Reflux Extraction (EHRE). HAYATI Journal of Biosciences, 29(4), 515-525. https://doi.org/10.4308/hjb.29.4.515-525
Section
Articles