Molecular Identification and Phylogenetic Analysis of Fungi Contaminants Associated with In Vitro Cultured Banana Based on ITS Region Sequence
Abstract
This study characterized, identified and conducted phylogenetic analysis on fungi contaminants in vitro bananas based on the sequence of inter-space (ITS) regions. Genomic DNA was extracted from the pure culture of fungi contaminants, amplified and sequenced using ITS1 and ITS4 markers. Analysis of the sequences using MEGA 7 Software at higher similarity sequence identified five Aspergillus spp., three Penicillium spp., one each of Fusarium, Trichoderma and Cladosporium as the contaminants. The genetic distance between the fungi species was 0.205, which suggests a homogeneous substitution between the sequences, and thiamine was the most stable. The fungi clustered in three major groups at 0.10 genetic distance, subdivided into five clusters. A cluster and sub-cluster consisting of five Aspergillus strains; a major cluster of three Penicillium strains; a cluster comprising of Fusarium chlamydosporum and Trichoderma viride; and a sole fungi Cladosporium tenuissimum. The Aspergillus group were phylogenetically related to A. flavus and A. parvissclerotigenus, the identified Penicillium spp. were closely related to Penicillium citrinum while the detected Cladosporium aligned with Cladosporium tenuissium and Phoma multirostrata. The information provided by this study could be utilized to develop a specific and compelling sterilization protocol to minimize the rate of contamination during in vitro culture procedures.
Downloads
Copyright (c) 2022 David Adedayo Animasaun, Chinaza Davies Nnamdi, Omotola I. Ipinmoroti, Stephen Oyedeji, Emmanuel A. Olonya, Ramar Krishnamurthy, Joseph Akintade Morakinyo
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
HAYATI J Biosci is an open access journal and the article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation. Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/