Antibacterial and Antioxidant Activities of Fungal Endophytes Isolated from Medicinal Plants in Simeulue Island, Aceh

Ahmad Fathoni; Muhammad Ilyas; Praptiwi; Dewi Wulansari; Andria Agusta

doi:10.4308/hjb.29.6.720-732

Antibacterial and Antioxidant Activities of Fungal Endophytes Isolated from Medicinal Plants in Simeulue Island, Aceh

Ahmad Fathoni Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, Bogor, Indonesia https://orcid.org/0000-0002-5412-0281
Muhammad Ilyas Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, Bogor, Indonesia
Praptiwi Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, Bogor, Indonesia https://orcid.org/0000-0003-1804-4549
Dewi Wulansari Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, Bogor, Indonesia https://orcid.org/0000-0003-1904-7741
Andria Agusta Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, Bogor, Indonesia

DOI: https://doi.org/10.4308/hjb.29.6.720-732

Abstract

Natural products from endophytic fungi have a wide range of medicinal applications, including antibacterial and antioxidant agents. The research aimed to evaluate the antibacterial and antioxidant activities of compounds derived from endophytic fungi isolated from medicinal plants collected from Simeulue Island by TLC dot-blot and microdilution assays. Eighty-one fungal extracts were screened for antibacterial and antioxidant activities. Antibacterial activity was evaluated against Gram-positive and negative bacteria, i.e., Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5, respectively. Antioxidant activity was carried out by using free radical scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH). The results of the TLC bioassay revealed that the fungal extracts have activities as antibacterial and antioxidant are 65 (against S. aureus), 57 (against E. coli), and 48 extracts (DPPH free radical scavenger). Two extracts, Phomopsis sp. 48BtSi-2.1 and Xylaria sp. 55DnSi-1.1, showed strong antibacterial activity against S. aureus with MIC value of 32 μg/ml. Furthermore, nine extracts (Schizophyllum sp. 20DnSi-1; Hyphomycetes 36BhSi-1.1; Phomopsis sp. 36DnSi-2.1; Schizophyllum sp. 39DnSi-1.1; Xylaria sp. 39RpSi-2.1; Phomopsis sp. 41BtSi-1.1; Phomopsis sp. 48BtSi-2.1; Lasiodiplodia sp. 48BtSi-3.1; Schizophyllum sp. 50DnSi-3) were strong activity against E. coli with MIC value ranges from 32 to 64 μg/ml. One extract, Xylaria sp. 04BtSi-2.2 has strong antioxidant activity as DPPH free radical scavenger with an IC₅₀ value of 21.66 μg/ml (AAI value of >1).

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Published

2022-06-28

How to Cite

FathoniA., IlyasM., Praptiwi, WulansariD., & AgustaA. (2022). Antibacterial and Antioxidant Activities of Fungal Endophytes Isolated from Medicinal Plants in Simeulue Island, Aceh. HAYATI Journal of Biosciences, 29(6), 720-732. https://doi.org/10.4308/hjb.29.6.720-732

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Vol. 29 No. 6 (2022): November 2022

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