Physalis angulata Leaf Ethanol Extract Reduces Oxidative Stress and Improves Endothelial Progenitor Cells in L-NAME-Induced Hypertensive Rats

  • Dian Nugrahenny Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Nur Permatasari Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Setyawati Soeharto Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Indriati Dwi Rahayu Department of Anatomy and Histology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Edwin Widodo Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Karyono Mintaroem Department of Anatomical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Hidayat Sujuti Department of Biomolecule and Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Retty Ratnawati Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Elly Mayangsari Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Dicky Faizal Irnandi Department of Biomolecule and Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Lavina Sofia Ardani Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Tika Ardhini Wardoyo Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Nathania Bella Claresta Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Nura Fattah Cantika Yoga Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Adinda Chika Anindita Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Niarti Ulan Sari Siarnu Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Fardizia Putri Alia Bachelor’s Degree Study Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

Abstract

This study aimed to evaluate the effects of ciplukan (Physalis angulata L.) leaf ethanol extract on L-NG-nitroarginine methyl ester (L-NAME)-induced hypertensive rats. We randomly divided twenty-five Wistar rats into five groups. The sham group was given a PBS injection. The hypertensive group was injected with L-NAME on days 1 to 28. Three groups of hypertensive rats were given the extract on days 4 to 28. Blood pressure was measured using the tail-cuff method on days 0, 4, 10, and 27. The endothelial progenitor cells (EPCs) in the blood were measured by flow cytometry as a percentage of circulating angiogenic cells (CACs, CD34+/CD309+/CD45+) and endothelial colony-forming cells (ECFCs, CD34+/CD309+/CD45-). Serum NO and MDA levels, as well as serum SOD activity, were measured colorimetrically. Serum TNF-α levels were measured by the ELISA method. The ciplukan leaf extract reduced systolic and diastolic blood pressure, reduced the percentage of EPCs in the blood, increased serum NO levels, reduced MDA levels, increased serum SOD activity, and reduced serum TNF-α levels in L-NAME-induced hypertensive rats. It is concluded that ciplukan ethanol leaf extract exerts protective effects on L-NAME-induced hypertensive rats. These study results can strengthen the scientific basis of using ciplukan leaf ethanol extract to treat hypertension.

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Published
2022-08-29
How to Cite
NugrahennyD., PermatasariN., SoehartoS., RahayuI. D., WidodoE., MintaroemK., SujutiH., RatnawatiR., MayangsariE., IrnandiD. F., ArdaniL. S., WardoyoT. A., ClarestaN. B., YogaN. F. C., AninditaA. C., SiarnuN. U. S., & AliaF. P. (2022). Physalis angulata Leaf Ethanol Extract Reduces Oxidative Stress and Improves Endothelial Progenitor Cells in L-NAME-Induced Hypertensive Rats. HAYATI Journal of Biosciences, 30(1), 81-87. https://doi.org/10.4308/hjb.30.1.81-87