Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production

  • Niken Respati Maharani 1Master Program, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta, Indonesia. 2Disease Investigation Center Subang, Ministry of Agriculture of The Republic of Indonesia, Subang, Indonesia
  • Heru Susetya Department of Veterinary Public Health, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia
  • Michael Haryadi Wibowo Department of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia

Abstract

Avian influenza virus H9N2 subtype is considered a low pathogenic strain that has been reported in Indonesia since late 2016. The outbreak has caused economic losses for farmers due to the sharp drop in egg production. The evidence of the existence of AIV H9N2 has been published, but very limited information on the prominent symptom and macroscopic lesion. This research was a retrospective study of suspected avian influenza H9 subtype, obtained from layer commercial farm with recorded characteristic symptoms. Specific trachea samples were collected and further processed to be isolated, propagated using embryonated chicken egg, and then extracted the RNA for molecular detection using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). A positive result was further detected in its H-9 gene with  RT-PCR technique and sequencing methods. One of five samples showed positive for RT-qPCR with CT value 30.19. Sequence analysis confirmed that the sample with characteristic macroscopic lesion could be detected in the presence of the AIV H9 subtype. Phylogenetic tree analyses revealed that this virus belongs to the China-Vietnam- Indonesia (CVI) lineage.

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Published
2022-04-20
How to Cite
MaharaniN. R., SusetyaH., & WibowoM. H. (2022). Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production. HAYATI Journal of Biosciences, 29(4), 531-539. https://doi.org/10.4308/hjb.29.4.531-539
Section
Articles