Protein Hydrolysate of Green Peas Bromelain Attenuates Kidney Fibrosis in Cisplatin-Induced Nephrotoxicity Rats: Emphasis on Anti-Inflammatory Activities

Meilinah Hidayat; Retno Pangastuti; Sijani Prahastuti; Khomaini Hasan

doi:10.4308/hjb.30.2.347-357

Protein Hydrolysate of Green Peas Bromelain Attenuates Kidney Fibrosis in Cisplatin-Induced Nephrotoxicity Rats: Emphasis on Anti-Inflammatory Activities

Meilinah Hidayat Nutrition Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia
Retno Pangastuti Student of Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia
Sijani Prahastuti Biochemistry Department, Faculty of Medicine,Universitas Kristen Maranatha, Bandung, Indonesia
Khomaini Hasan Biochemistry Department, Faculty of Medicine, Universitas Jendral Ahmad Yani, Cimahi, Indonesia https://orcid.org/0000-0002-7395-4206

DOI: https://doi.org/10.4308/hjb.30.2.347-357

Abstract

Massive tubule fibrosis is a histopathology hallmark of chronic renal failure (CRF). The previous study of protein hydrolysate of green pea (Pisum sativum) bromelain (PHGPB) showed promising results as an antifibrosis in glucose-induced renal mesangial culture cells, by decreasing their TGF-β levels. In this experiment the anti-inflammatory, anti-fibrotic effect of PHGPB in Cisplatin-induced chronic renal failure (CRF) rats was measured. The purpose of this study is to evaluate the anti-inflammatory and antifibrosis effect of PHGPB in Cisplatin-induced chronic renal failure rats. Five groups consisted of five rats: negative control, Cisplatin control, and three groups of Cisplatin+PHGPB (dose of 100, 200, and 400 mg/kg BW/day) treated for 56 days. The examined parameters are Beta-2-microglobulin (β2MG), hs-CRP, histopathological observations of Masson's trichrome stain, and IHC. The level of β2MG and hs-CRP in the Cisplatin+PHGPB group lowered and was highly different from Cisplatin control (p<0.05) depending on doses. In histopathology, the Cisplatin+PHGPB 400 mg/kg BW group showed less fibrosis and no significant difference with the negative control in Masson trichome staining. While in IHC histopathology, PHGPB treatment slightly ameliorated TGF-β expression and intensity of TGF-β values convincingly. In conclusion, PHGPB can relieve kidney fibrosis in cisplatin-induced nephrotoxicity rats: Emphasis on anti-inflammatory activities.

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Published

2022-12-21

How to Cite

HidayatM., PangastutiR., PrahastutiS., & HasanK. (2022). Protein Hydrolysate of Green Peas Bromelain Attenuates Kidney Fibrosis in Cisplatin-Induced Nephrotoxicity Rats: Emphasis on Anti-Inflammatory Activities. HAYATI Journal of Biosciences, 30(2), 347-357. https://doi.org/10.4308/hjb.30.2.347-357

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Vol. 30 No. 2 (2023): March 2023

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