The Potential Roles of Gut Microbiome in Modulating the Immune Response of Asian Redtail Catfish (Hemibagrus nemurus) Vaccinated with Aeromonas hydrophila
The research aims are to observe the effect of vaccination in microbial profiles and gut microbiome composition. The treatments were as follows: the fishes were injected with PBS and challenged (A); the fishes were injected with freeze–dried vaccine dissolved in 100 ml 0.85% NaCl and challenged (B); the fishes were injected with freeze–dried vaccine dissolved in 50 ml 0.85% NaCl and challenged (C), and the fishes were injected with liquid vaccine and challenged (D). Microbiome composition measurements were carried out on the 21st–day post–vaccination and the 7th day after the challenge test. Fish intestine samples from three replications were tested by Next Generation Sequencing (NGS). Two significant phyla were identified from all treatments, namely Proteobacteria and Firmicutes. Cetobacterium, Candidatus Bacilloplasma, and Clostridium sensu stricto were the genera classified as good bacteria in vaccinated fish. It can be concluded that vaccination can increase the diversity of the gut microbiome, especially bacteria beneficial to the fish host. Chitosan as a coating antigen in freeze–dried vaccine increases gut microbiome's number and diversity better than a liquid vaccine.
Copyright (c) 2022 Septyan Andriyanto, Yani Aryati, Tuti Sumiati, Angela Mariana Lusiastuti, Nurhidayat, Kurniawan Kurniawan, Rofingah Kuswatun, Nining Nuraeni
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
HAYATI J Biosci article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation.
Authors who submit and publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/
licenses/by-nc/4.0/Attributi on — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- Authors can still use their work commercially
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).