Genipin Crosslinked Blended Collagen-Chondroitin: A Promising Biomaterial Scaffold Candidate for Cartilage Reconstruction

  • Trimartani Trimartani Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia https://orcid.org/0000-0002-7440-0950
  • Normalina Sandora Indonesia Medical Education and Research Institute, Faculty of Medicine University of Indonesia, Jakarta, Indonesia
  • Bambang Hermani Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Jeanne A Pawitan Department of Histology, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Raden Ayu Anatriera Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Abstract

Tissue engineering offers a solution to the shortage of materials for cartilage reconstruction surgery by providing various potential biomaterial scaffolds. Tissue engineering utilizes biological or synthetic biomaterials as a scaffold for the host cells to repopulate and regenerate the tissue. The natural biomaterials such collagen and chondroitin imitates native cartilage matrix composition. Genipin as one of natural crosslinkers was added to improve the matrix biomechanical properties. This study was done to investigate biocomposition of blended collagen type 1, collagen type 2, chondroitin sulphate (Col1-Col2-CS) and genipin for its cytotoxicity using human umbilical mesenchymal stem cells (hUCMSCs), surface morphology, and biochemical composition. Genipin-crosslinked collagen-chondroitin biocomposite showed a homogeneous shape while uncrosslinked biocomposite had rough surface and fibrillar folds size. Spectroscopy demonstrated both biocomposites had similar peak resemble to no alternation of the biocomposition by crosslinking. Both types of biocomposites were biocompatible and had no toxic effects, as compared to the cell colony only (p value = 0.26). The conclusion are blended composite of collagen chondroitin crosslinked with genipin had generated a fine microstructure scaffold with smaller pore size, had similar biomolecular component spectrum absorption, and no exhibition of residual toxicity.

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Author Biographies

Trimartani Trimartani, Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Normalina Sandora, Indonesia Medical Education and Research Institute, Faculty of Medicine University of Indonesia, Jakarta, Indonesia

Indonesia Medical Education and Research Institute, Faculty of Medicine University of Indonesia, Jakarta, Indonesia 

Bambang Hermani, Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Jeanne A Pawitan, Department of Histology, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Department of Histology, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Raden Ayu Anatriera, Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Published
2022-01-03
How to Cite
TrimartaniT., SandoraN., HermaniB., PawitanJ. A., & AnatrieraR. A. (2022). Genipin Crosslinked Blended Collagen-Chondroitin: A Promising Biomaterial Scaffold Candidate for Cartilage Reconstruction. HAYATI Journal of Biosciences, 29(2), 122-128. https://doi.org/10.4308/hjb.29.2.122-128
Section
Short Communication