Application of Proteomics to Identify Fertility Markers in Angus Bull Sperm

Naseer A.  Kutchy; Sule  Dogan; Xiaojun  Wang; Einko  Topper; Abdullah  Kaya; Erdogan  Memili

doi:10.4308/hjb.27.2.116

Application of Proteomics to Identify Fertility Markers in Angus Bull Sperm

Naseer A. Kutchy Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State
Sule Dogan Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State
Xiaojun Wang Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State
Einko Topper Alta Genetics, Inc., Watertown
Abdullah Kaya Alta Genetics, Inc., Watertown
Erdogan Memili Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State

DOI: https://doi.org/10.4308/hjb.27.2.116

Abstract

The goal of the study was to ascertain sperm proteins as fertility markers by identifying sperm proteins in Angus bull sperm using proteomics and validate the markers through comparative sperm biology between Angus and Holstein bulls for which there is reliable fertility data available. We aimed to determine proteins differentially expressed in sperm from Angus bulls with different fertility phenotypes. Two-dimensional differential gel electrophoresis with mass-spectrometry, functional gene clusters, canonical pathways and protein networks, using integrated discovery bioinformatics software and ingenuity pathway analysis were used to identify and analyze sperm proteome. We identified 80 proteins that were differentially expressed in sperm of our experimental population. Using computational biology approaches we demonstrated involvement of structural proteins such as outer dense fiber of sperm tails 2 and enzymes including kinases, and phosphatases having functions in essential pathways in glycolysis/gluconeogenesis and free scavenging. The results are significant because analyzed proteins in Angus sperm are determinants of fertility, gene-environment interactions, as well as potential biomarkers for animal breeding.

Downloads

Download data is not yet available.

Published

2020-04-01

How to Cite

KutchyN. A., DoganS., WangX., TopperE., KayaA., & MemiliE. (2020). Application of Proteomics to Identify Fertility Markers in Angus Bull Sperm. HAYATI Journal of Biosciences, 27(2), 116. https://doi.org/10.4308/hjb.27.2.116

Download Citation

Issue

Vol. 27 No. 2 (2020): April 2020

Section

Articles

HAYATI J Biosci article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation.

Authors who submit and publish with this journal agree to the following terms:

Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/licenses/by-nc/4.0/ Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Authors can still use their work commercially
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).