Purification and Characterization of ?-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei
AbstractTrichoderma enzymes that inhibit fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal root rot pathogen Ganoderma philippii. This experiment was aimed to purify and characterize the ?-1,3-glucanase of T. reesei. Extracellular ?-1,3-glucanase was produced by growing mycoparasite T. reesei isolate T13 in colloidal chitin and sucrose as carbon sources. The enzyme was then purified to its homogeneity by precipitation with ammonium sulfate, followed by gel filtration chromatography and chromatofocusing. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 12% was used to confirm the purity of enzyme at each stage of preparation and to characterize purified protein. The results showed that T. reesei produced at least three extracellular ?-1,3-glucanases. Estimation of molecular weight based on SDS-PAGE 12% have three isoform of ?-1,3-glucanase were 90 kDa for ?-1,3-glucanase-I, 75 kDa for ?-1,3-glucanase-II, and 64 kDa for ?-1,3-glucanase-III. Their optimum pH and temperature were 5 and 50 oC, respectively.
Key words: ?-1,3-glucanase, Trichoderma reesei, Ganoderma philippii
Starting Vol. 25 No. 1, HAYATI J Biosci article's license is CC-BY. This license lets others distribute, remix, tweak, and build upon author's work, even commercially, as long as they credit the original creation.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).