Expression Study of Banana Pathogenic Resistance Genes

  • Fenny M. Dwivany School of Life Sciences and Technology, Institut Teknologi Bandung ForMIND Institute Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Bandung
  • Rizkita Rahmi Esyanti School of Life Sciences and Technology, Institut Teknologi Bandung Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Bandung
  • Aksarani 'Sa Pratiwi School of Life Sciences and Technology, Institut Teknologi Bandung ForMIND Institute
  • Herafi Zaskia School of Life Sciences and Technology, Institut Teknologi Bandung ForMIND Institute
DOI: https://doi.org/10.4308/hjb.23.4.196
Keywords: apoptosis inhibitor 5 (API5), Arabidopsis thaliana, chitinase (ChiI1), Musa acuminata, pathogen resistance

Abstract

Banana is one of the world's most important trade commodities. However, infection of banana pathogenic fungi (Fusarium oxysporum race 4) is one of the major causes of decreasing production in Indonesia. Genetic engineering has become an alternative way to control this problem by isolating genes that involved in plant defense mechanism against pathogens. Two of the important genes are API5 and ChiI1, each gene encodes apoptosisinhibitory protein and chitinase enzymes. The purpose of this study was to study the expression of API5 and ChiI1 genes as candidate pathogenic resistance genes. The amplified fragments were then cloned, sequenced, and confirmed with in silico studies. Based on sequence analysis, it is showed that partial API5 gene has putative transactivationdomain and ChiI1 has 9 chitinase family GH19 protein motifs. Data obtained from this study will contribute in banana genetic improvement.

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Published
2018-03-23
Issue
Vol. 23 No. 4 (2016): October 2016
Section
Articles

Copyright (c) 2018 Fenny M. Dwivany, Rizkita Rahmi Esyanti, Aksarani 'Sa Pratiwi, Herafi Zaskia

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