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Vol 31, No 2 (2008): Forum Pascasarjana

Silica, a polimerized silicon dioxide, is widely used as raw materials for food industries, such as food packaging, filter agent, biomarkers and biosensor for various analysis.  In biological sistem such as sponge, the formation of silica structure was directed by protein known as silicatein.  The aims of this research were to extract silicatein-like protein isolated from sponge live surrounding the Nias and Lombok seacost Indonesia and to study their activity to polymerize tetraethoxyorthosilicate (TEOS) in-vitro.  Protein in silica spicule was isolated by collecting silica spicule, soaked in HF/NH4F buffer (pH5.0) for dissolving silica and releasing this protein.  The protein was analysed by electrophoresis SDS-PAGE to estimate the molecular weight and the concentration was analyzed by Bradford method.  The highest yield of silica spicula was 58.5% of dry weight sponge that was isolated from sponge MT37.  By SDS-PAGE, the molecular weight of protein from N6 showed three bands of 32, 27, 23 kDa, while MT5 protein was 15.5 kDa, and MT37 protein was 18 kDa.  The highest polymerization activity was 144 µmol/ml TEOS occurs at 12 hours, showed by protein isolated from sponge MT37 of Lombok Marine.

 

Key words: sponge, silicatein like-protein, tetraethoxyorthosilicate
Abstract   PDF
M.R.R. Lukie Trianawati, Maggy T. Suhartono, Dahrul Syah, Ekowati Chasanah
 
Vol 35, No 1 (2012): Forum Pascasarjana Reconstruction of The Inner Baduy’s Ecohouse Structure in Banten Province Abstract   PDF
Meiske Widyarti, Budi Indra Setiawan, Hadi Susilo Arifin, Arief Sabdo Yuwono
 
Vol 14, No 1 (1991): Forum Pascasarjana Response of Soybean (Glycine max (L.) Merr.) to Micronutrients Zn, Cu, B at Some Dosages of Manure on Latosol Soil Abstract   PDF
Maya Melati, Fred Rumawas, Justika S Baharsjah, IPG Widjaja Adhi
 
Vol 35, No 1 (2012): Forum Pascasarjana The Economic Impact of International Tourism on the Indonesian Economy Abstract   PDF
Adi Lumaksono, D.S. Priyarsono, Kuntjoro ., Rusman Heriawan
 
Vol 14, No 1 (1991): Forum Pascasarjana The Effect of Phosphorus Fertilization and Variety on The Growth and Production of Soybean (Glycine m a (L.) Merr) Under Saturated Soil Culture Abstract   PDF
Munif Ghulamahdi, Fred Rumawas, Joedojono Wiroatmodjo, Jajah Koswara
 
Vol 18, No 1 (1995): Forum Pascasarjana The Estimation of Price Sensitivity Curves Using Generalized Linear Models Abstract   PDF
Hari Wijayanto, Khairil A. Notodiputro, . Barizi, Jajah K. Wagiono
 
Vol 16, No 1 (1993): Forum Pascasarjana The Genetic Estimation of Fruit Component Parameters of Seven Coconut Populations Without a Progeny Test Abstract   PDF
Dwi Asmono, Alex Hartana, Edi Guhardja, Sudirman Yahya
 
Vol 35, No 1 (2012): Forum Pascasarjana The Impact of Government Policy of Presidential Instruction in 2005-2008 for Rice Policy on Food Security) Abstract   PDF
Surya Abadi Sembiring, Harianto Harianto, Hermanto Siregar, Bungaran Saragih
 
Vol 32, No 4 (2009): Forum Pascasarjana The research objective was to study tree's vegetation structure based on diameter class distribution on varians vegetation types at Salak Mountain.  Samples were taken at north, south, east, and west facing slope of Salak Mountain using line transect.  Systematic sampling with random start was used to locate the transetcs.  Measuring stem diameter at breast height was done in order to study the tree diameter class distribution.  Non parametric U Man Whitney statistic was used to know whether there was a different in number of individual at all diameter class in each vegetation type.  At mix forest and plantation forest, the tree diameter class distribution forming J curve shape.  At bamboo forest, the individual number increase at the highest class diameter.  The number of individual trees were highest at mix forest and lowest at bamboo forest. Key words: Mount Salak, Reverse J Curve, tree diameter class,  vegetation type Abstract   PDF
Muhammad Wiharto, Cecep Kusmana, Lilik Budi Prasetyo, Tukirin Partomihardjo
 
Vol 34, No 3 (2011): Forum Pascasarjana Urban pests like cockroaches, mosquitoes, and flies can transmit human diseases that cause health problems in the society.  Currently, mosquito is main insect that transmit very deadly diseases, such as dengue fever, cikungunya, and malaria. Prevention of those diseases is generally focused on controlling the vectors in various ways, such as fogging, the use home pesticides, sanitation, use of mosquito repellents, electric rackets, and other traditional ways developed by the community (mosquito net, gauze, water).  The purpose of this study was to describe the relationship between characteristics of peoples live in Jakarta (based on age, sex, education, and income level) to their behavior in using home pesticides and to know the impact of those pesticides to peoples and pets live in their houses.  Research sites were purposively directed to the areas that were appropriate with the substances and purposes of this study.  Interviewed was conducted to the respondents (housewives) and inspection of their houses were also conducted to classify their houses as clean, moderately clean, and dirty or slum.  Based on a survey to 155 respondents, it was revealed that respondent characteristics as well as their house conditions influenced the behavior of those respondents in choosing home pesticides.  Majority of  housewives (28.21%) use liquid pesticides which were contains the active ingredient of cypermethrin 0.4 g/l, imiprotrin 0.32 g/l, and transflutrin 0.2 g/l.  In addition, 47.26% of them ignored the instruction written on the label in using those pesticides.  Awareness of those respondents was still low in terms of dumping the package of pesticides; 96.64% of respondents dumped pesticides to the dumpster near their houses.  Commercial advertencies from electronic media, especially TV, influence half of the respondents (52.74%) in choosing home pesticides used.  In general, it can be summarized that the awareness of respondents in using home pesticides were still low.  Most housewives did not know the danger of those chemicals and how to use it properly.  Only 38.46% of them used non-chemical methods, such as insect net.   Key words: home pesticides, active ingredient, urban pest, housewife, the environment Abstract   PDF
Titiek Siti Yuliani, Hermanu Triwidodo, Kooswardhono Mudikdjo, Nurmala K. Panjaitan, Sjafrida Manuwoto
 
Vol 13, No 2 (1990): Forum Pascasarjana Variability Analysis Of Several Soybean Mutants And Varieties II. Electrophoretic Study Of Seed Globulin And Albumin Abstract
M Jusuf, N Marina, U Widyastuti, A Girindra
 
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