Darusman, Latifah K, Indonesia

  • Forum Pasca Sarjana Vol. 34 No. 1 (2011): Forum Pascasarjana - Articles

    A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute).  The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes.  However, only some of pollutant (Cu, Zn, Pb and E.coli) can be reduced until below tolerable threshold.


    Keywords: effluent, leachate, tolerable threshold

    Abstract  PDF
  • Forum Pasca Sarjana Vol. 33 No. 1 (2010): Forum Pascasarjana - Articles

    The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis.  Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s monocytes.  Analyses were done by measuring the formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde (MDA).  LDL were harvested and isolated from 5 adult female Macaca fascicularis  fed aterogenic diet for 3 months.  LDL oxidation by mice macrophage incubated for 4 hours were inhibited by curcuminoid at concentration of 8 ppm.  There was decreased 17% (P<0.01) in the concentration of MDA compared to control without curcuminoid (31.99B±0. vs 36.77A±0.9 nmol/mg protein LDL, respectively).  Inhibiton of LDL oxidation in M. nemestrina macrophage were highest by curcuminoid at 8 ppm for 4 hours and 6 hours incubation.  There was 14.8% and 23% inhibiton (P<0.01) (23.768 ± 0.095A vs 27.111B±0.972 and 23.37B±0.12 vs 30.87A±2.49 nmol/mg LDL protein, respectively).  These data suggest that curcuminoid of temu mangga were able to inhibit LDL oxidation in the cellular level, therefore offer protection against oxidation of LDL.


    Key words: LDL, atherosclerosis, curcuminoid, Curcuma mangga
    Abstract  PDF