Perbandingan Pertumbuhan <i>Butyrivibrio fibrisolvens</i> E14 Varian <i>Sticky</i> dan <i>Loose</i>

  • Djarot Sasongko Hami Seno Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
  • John Douglas Brooker


A number of Butyrivibrio fibrisolvens strains have been reported to attach cellulose fibres, feed and other particles in the rumen, as well as to sheep rumen epithelia. In this research, B. fibrisolvens E14 variants sticky (S) and loose (L) were used to study the mechanism of B. fibrisolvens attachment to surfaces, especially to plant fibre. Results may be useful in enhancing plant fiber degradation within the rumen, or the use of plant biomass as energy source. The two cell types were compared; studies included physical and growth characteristics in defined, solid or liquid medium containing various carbon sources, the presence of compounds that may induce or inhibit attachment, and their phenotypic stability. Compared to the non-adhering L cells, the adhering S cells were shinier, spherical, more intensely pigmented (yellow), more firmly attached to the agar surface and could only be removed with scraping. After longer incubation, the cells were released from the agar but the colonies tended to stick together, and only became separable when further incubated. In contrast, the L cells were non spherical, loosely attached to the agar and separable at all stages of growth. In liquid medium, the S cells tended to clump during the early stages of growth, and be dispersed at later stages. The L cells were dispersed throughout the medium at all stages of growth. The phenotypes of the 2 variants were stable; both variants maintained their characteristics through multiple passages on solid and in liquid medium. The presence of molecules that induced attachment of S or inhibited attachment of L cells were not detected.


Butyrivibrio fibrisolvens; bacterial attachment/adhesion; rumen; plant fiber degradation Banner MA, Cunniffe JG, Macintosh RL, Foster TJ, Rohde H, Mack D, Hoyes E, Derrick J, Upton M, Handley PS (2007) Localized tufts of fibrils on Staphylococcus epidermidis NCTC 11047 are comprised of the accumulationassociated protein. J. Bacteriol. 189: 2793–2804.

Bauchop T, Clarke R T J, Newhook J C (1975) Scanning electron microscopy study of bacteria assosiated with the rumen ephitelium of sheep. Appl. Microbiol. 30: 668-675

Bauchop T (1980) Scanning electrone microscopy in the study of microbial digestion of plant fragments in the gut. In: Ellwood D C, Hedger J N, Latham M J, Lynch J M, Slater J H (eds.), Contemporary microbial ecology, Academic Press, New York, pp. 305-326

Bauchop T (1981) The anaerobic fungi in rumen fibre digestion. Agric. Environ. 6: 338-348

Beard C F, Hefford M A, Forster R J, Sontakke S, Teather R M, Gregg K (1995) A stable and efficient transformation system for Butyrivibrio fibrisolvens OB156. Curr. Microbiol. 30:105-109

Bowden MG, Chen W, Singvall J, Xu Y, Peacock SJ, Valtulina V, Speziale P, Hook M (2005) Identification and preliminary characterization of cell-wall anchored proteins of Staphylococcus epidermidis. Microbiol. 151: 1453–1464.

Byrant M P (1959) Bacterial species of the rumen. Bacteriol. Rev. 23: 125-153

Corrigan RM, Rigby D, Handley P, Foster TJ (2007) The role of Staphylococcus aureus surface protein SasG in adherence and biofilm formation. Microbiol. 153: 2435–2446.

Dehority B A (1975) Characterization studies on rumen bacteria isolated from Alaskanreindeer (Rangifer tarandus L.). Proc. of the first International Reindeer and Caribou Symposium, University of Alaska, Anchorage, 228-240

Dehority B A, Grubb J A (1977) Characterization of the predominant bacteria occuring in the rumen of goats (Capra hircus). Appl. Environ. Microbiol. 33: 1030-1036

Dehority B A, Grubb J A (1981) Bacterial population adherent to the ephitelium on the roof of the dorsal rumen of sheep. Appl. Environ. Microbiol. 41: 1424-1427

Dinsdale D, Jane Morris D, Bacon S D (1978) Electron microscopy of the microbial populations present and their modes of attack on various cellulosic substrates undergoing digestion in the sheep rumen. Appl. Environ. Microbiol. 36: 160-168

Fletcher M (1977) The effects of culture concentration and age, time and temperature on bacterial attachment to polystyrene. Can. J. Microbiol. 23: 1-6.

Fletcher M (1980a). Adherence of marine microorganisms to smooth surfaces. In: Beachey E H (ed.), Bacterial adherence. Chapman and Hall, New York.

Fletcher M (1980b) The question of passive versus active attachment mechanisms in nonspecific C. W., Lynch, J. M., Melling, J., Rutter, P. R. and Vincent, B. (eds.), Microbial adhesion to surfaces, Ellis Horwood, pp. 197-210

Flint HJ, Bayer EA (2008) Plant cell wall breakdown by anaerobic microorganisms from the mammalian digestive tract. Ann. New York Acad. Sci. 1125: 280–288.

Fujino T, Beguin P, Aubert JP (1993) Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein CipA and protein possibly involved in attachment of the cellulosome to the cell surface. J. Bacteriol. 175: 1891-1899

Gelhaye E, Petitdemange H, Gay R (1993) Adhesion and growth of Clostridium cellulolyticum ATCC 35319 on crystalline cellulose. J. Bacteriol. 175:3452-3458

Gerngross V T, Romaniac P M, Huskisson N S, Demail A L (1993) Sequencing of Clostridium thermocellum gene (cipA) encoding the cellulosomal SL protein reveals an unusual degree of internal homology. J. Mol. Biol. 8: 325-334

Hespell R B (1991) The genera Butyrivibrio, Lachnospira, and Roseburia. In: Balows A, Truper H G, Dworkin M, Harder W, Schleifer K H (eds.), The Procaryotes: a hand book on the biology of bacteria. Ecophysiology, isolation, identification, and application. Springer-Verlag, New York, pp. 2022-2023

Hespell R B, Cotta M A (1995) Degradation and utilization by Butyrivibrio fibrisolvens H17c of xylans with different chemical and physical properties. Appl. Environ. Microbiol. 61: 3042-3050

Hungate R E (1988) The ruminant and the rumen. In: Hobson, P. N. (ed.), The rumen microbial ecosystem, Elsevier Applied Science, London and New York, pp. 1-20.

Izano EA, Amarante, MA, Kher WB, Kaplan JB (2008) Differential roles of poly-Nacetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms. Appl. Environ. Microbiol. 74: 470–476.

Krause DO, Denman SE, Mackie RI, Morrison M, Rae AL, Attwood GT, McSweeney CS (2003) Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics. FEMS Microbiol. Rev. 27: 663–693

Margherita SS, Hungate R E (1963). Serological analysis of Butyrivibrio from the bovine rumen. J. Bacteriol. 86: 855-860

Marshall K C, Cruickshank RH (1973) Cell surface hydrophobicity nd orientation of certain bacteria at interfaces. Arch. Microbiol. 91: 29-40.

Mead L J, Jones G A (1981) Isolation and presumptive identification of adherent epithelial bacteria (“epimural”) bacteria) from the ovine rumenwall. Appl. Environ. Microbiol. 41: 1020- 1028.

Miron J, Ben-Ghedalia D (1993) Digestion of cell wall-wall monosaccharide of ryegrass and alfalfal hays by ruminal bacteria Fibrobacter succinogens and Butyrivibrio fibrisolvens. Can. J. Microbiol. 39: 780-786

Miron J, Duncan S H, Stewart C S (1994) Interaction between rumen bacterial strains during degradation and utilization of the monosaccharide of barley straw cell walls. J. Appl. Bacteriol. 76: 282-287

Miron J, Jacobovitch J, Bayer E A, Lamed R, BenGhedalia D (2001) Subcellular distribution of glycanases and related components in Ruminococcus albus SY3 and their role in cell adhesion to cellulose. J. Appl. Microbiol. 91: 677-685.

Moore W E C, Holdeman L V (1974) Human fecal flora: the normal flora of 20 Japanese Hawaiians. Appl. Microbiol. 27: 961-979.

Moore W E C, Johson J L, Holdeman L V (1976) Emendation of Bacteroidaceae and Butyrivibrio and description of Desulfomonas gen nov. and the new species in the genera Desulfomonas, Butyrivibrio, Eubacterium, Clostridium, and Ruminococcus. Int. J. Syst. Bacteriol. 26: 238-252.

Nili N, Brooker J D (1995) A defined medium for rumen bacteria and identification of strains impaired in de novo biosynthesis of certain amino acids. Lett. Appl. Microbiol. 21: 69-74.

Nili N (1996) Limitations to amino acid biosynthesis in ruminal strains of Prevotella and Butyrivibrio. Ph. D. thesis, University of Adelaide, South Australia, Chapters: 1, 2, 6, 7.

Orpin C G, Mathiesen S D, Greenwood Y, Blix A S (1985) Seasonal changes in the ruminal microflora of the high–artic Svalbard reindeer (Rangiver tarandus platyrhyncus). Appl. Environ. Microbiol., 50: 144-151.

Ørstarvik D (1977) Sorption of Streptococci to glass: effect of macromolecular solutes. Acta Path. Microbiol. Scand. Sect. B. 85: 47-53.

Rasmussen M A, White B A, Hespell R B (1989) Improved assay for quantitatin adherence of ruminal bacteria to cellulose. Appl. Environ. Microbiol. 55: 2089-2091.

Rice KC, Mann EE, Endres JL,Weiss EC, Cassat JE, Smeltzer MS, Bayles KW (2007) The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus. Proc. Natl. Acad. Sci. USA, 104: 8113–8118.

Rohde H, Burandt EC, Siemssen N, Frommelt L, Burdelski C, Wurster S, Scherpe S, Davies AP, Harris LG, Horstkotte MA (2007) Polysaccharide intercellular adhesin or protein factors in biofilm accumulation of Staphylococcus epidermidis and Staphylococcus aureus isolated from prosthetic hip and knee joint infections. Biomaterials, 28: 1711–1720.

Rohde H, Burdelski C, Bartscht K, Hussain M, Buck F, Horstkotte MA, Knobloch JK, Heilmann C, Herrmann M, Mack D (2005) Induction of Staphylococcus epidermidis biofilm formation via proteolytic processing of the accumulation-associated protein by staphylococcal and host proteases. Mol. Microbiol. 55: 1883–1895.

Rumney C J, Duncan S H, Henderson C, Stewart C S (1995) Isolation and characteristics of a wheatbran-degrading Butyrivibrio from human faeces. Lett. Appl. Microbiol. 20: 232-236.

Stack R J (1988) Neutral sugar composition of extracellular polysaccharides produced by strains of Butyrivibrio fibrisolvens. Appl. Environ. Microbiol. 54: 878-883.

Thareja A, Puniya AK, Goel G, Nagpal R, Sehgal JP, Singh PK, Singh K (2006) In vitro degradation of wheat straw by anaerobic fungi from small ruminants. Arch. Anim. Nutr. 60: 412–417.

van Gylswyk N O (1976) Some aspects of the metabolism of Butyrivibrio fibrisolvens. J. Agric. Sci. 97: 105-111.

Waters CM, Bassler BL (2005) Quorum sensing: cell-to-cell communication in bacteria. Annu. Rev. Cell. Dev. Biol. 21: 319–346.

Zhang Y, Gao W, Meng Q (2006) Fermentation of plant cell walls by ruminal bacteria, protozoa and fungi and their interaction with fibre particle size. Arch. Anim. Nutr. 61: 114–125.