Suseno, Rusmilah, Indonesia
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Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 1 (1999): Buletin Hama dan Penyakit Tanaman - Articles
Geminivirus infecting pepper: symptom variation and transmission study Infection of geminiviruses has been reported to cause signz3cantyield loss on various crops. Polymerase chain reaction was able to detect geminivirus infection on pepper samples around Bogor and Cipanas, West Java. Symptom variation and transmission of the geminivirus infectingpepper was studied using five solanaceae plants with three dzgerent transmission mannec i.e. mechanical inoculation, side grafirng, and insect vector. The transmission study showed that mechanical inoculation was not able to transmit the eminivirus, while side grafting and insect vector, Bemisia tabaci, caused infection with symptoms varies from yellow mosaic, leaf curl leaf distortion, to stunting of the plant. Higher infection was observed on pepper var. Hot Chilli through B. tabaci (8009) and side grafting (71.4%) than those on chilli pepper and tomato. DNA fiagment of - 1.7 kb was amplified wing PCRfim those plants showing symptoms, but no DNA fiagment was observedfrom symptomless eggplant and tobacco var. White Burley. Thus it can be concluded that the last two kina3 ofplants were resistant to the geminivirus infectingpepper.
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Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman - Articles
It has been known that phytoplasma inducing witches broom disease can infect various kind of leguminous plants in Indonesia, among others soybean. Information on the resistance of various kind of soybean varieties to the phytoplasma is still limited. Experiments were conducted to eveluate the resistance of ten soybean varieties/lines to the phytoplasma. The evaluation was carried out by inoculation method through Orosius argentatus Evans, with two days of acquisition feeding period, ten days latent period and two days inoculation feeding period. Observation was conducted on the presence of phytoplasma, incubation period, symptom and number & weight of seed. The result indicated that line Malang 3474 was resistant, Sindiro and Sriono were tolerant, while the other tested varieties/lines, i.e. Galunggung, Orba, Ringgit, Wilis, Malang 2999, Malang 2805 and Sicinang were susceptible
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Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman - Articles
Biological and molecular variability among PStV (peanut stripe virus) strains isolated from various peanut growing areas in Indonesia was studied based on their pathogenicity and coat protein genes. Six strains of PStV causing different types of symptoms on peanut leaves were used to study biological and molecular variability among these strains. Biological variability of the PStV strains was evaluated based on their pathogenicity on peanut cultivars, Gajah and Landak. While analysis of the coat protein gene was used to study the molecular variability of PStV strains. Based on their pathogenicity, six PStV strains could be grouped into three groups, medium, strong, and very strong strains. Analysis of a coat protein gene showed the variability of the PStV strains were 0-2.1percent based on nucleotide sequence and 0-1.4percent based on predicted amino acid sequence. There was no correlation between biological and molecular variability.
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Buletin Hama dan Penyakit Tumbuhan Vol. 12 No. 2 (2000): Buletin Hama dan Penyakit Tanaman - Articles
Resistance of peanut varieties to phytoplasm. Recently, witches' broom disease on peanut is becoming more important in Indonesia The use of resistant varieties is very potential to overcome the yield loss caused by the disease. Ten peanut varieties were evaluated for their response to phytoplasma using insect vector transmission. Three categories of plant response were observed during the experiment, i.e. moderate resistant (var, Macan, Zebra, and Simpai), susceptible (var. Biawak, Treggiling, Pelanduk, and Kidang), very susceptible (var. Gajah, Tupai, and Banteng). The average seed weight decrease per plant due to the phytoplasma infection was 40.99 - 100%.
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