Spesies Nematoda Puru Akar (Meloidogyne spp.) yang Berasosiasi dengan Penyakit Umbi Bercabang pada Wortel: Penyakit Baru di Indonesia

  • . Supramana
  • Gede Suastika
Keywords: carrots, root branching, species Melidogyne

Abstract

Diseases and pests is one of the main obstacles in the cultivation of carrots in Indonesia. One of the emerging diseases that cause significant losses is branched (forked) tuber caused by root knot nematodes (RKN), Meloidogyne spp. Research aimed to identify the species of RKN was carried out by taking samples of diseased plants and soil from the carrot plantation in four vegetable production centers on the island of Java, namely: (1) District Pacet, Cianjur, West Java, (2) Dieng Plateau, Central Java, (3) Kopeng, Central Java and (4) District Bumiaji, Kota Batu, East Java. Three carrot plantations with different altitude / elevation were sampled for each area involved. NPA species identification was done by observation of perineal pattern (fingerprint-like pattern) of females and PCR of nematodes r-DNA ITS. Four Meloidogyne species, namely M. arenaria, M. hapla, M. incognita, and M. jananica, were identified from West Java, Central Java and East Java samples, whereas M. fallax found only in West Java.

Downloads

Download data is not yet available.

Author Biographies

. Supramana

Departemen Proteksi Tanaman, Fakultas Pertanian, Institut
Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

Gede Suastika

Departemen Proteksi Tanaman, Fakultas Pertanian, Institut
Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

References

Barker KR, Campbell CL. Sampling nematode population. Di dalam: Zuckerman BM and Rohde RA, editor. Plant Parasitic Nematodes. Vol. III. New York: Academic Press.

[BPS] Biro Pusat Statistik. 2008. Statistik Indonesia 2008. BPS. Jakarta. Indonesia. Hlm 200-205.

Davis MR. 1981. Carrot disease and their mana-gement. Di dalam: Naqvi SAM, editor. Diseases of Fruits Vegetables Diagnosis and Management. Ed ke-1. USA: Kluwer Academic Publisher.

Davis ME, Sorensen E, Nunez J. 2005. Crop profile for carrots in the United States. http:// pestdata.ncsu.edu/cropprofiles/Detail.CFM FactSheets_ RecordID =167.

Eisenback JD, Triantaphyllou HH. 1991. Root-knot nematode: Important nematode parasite. In: Kinloch RA, Barker KR, Pederson GA, Windham GL, editor. Plant and Nematode Interactions. USA: ASA, CSSA, SSA, Publishers.

Eisenback JD, Hirschmann H, Sasser JN, and Triantaphyllou HH. A Guide to the Most Common Species of Root-knot Nematodes (Meloidogyne species), with a Pictorial Key. International Meloidogyne Project, Dept. Plant Pathol. NC State Univ. Raleigh.

Ferris H. 2008. Meloidogyne hapla. http://www. nematode.unl.edu/mhap.htm [20 October 2009].

Hay F. 2005. Nematode control in carrots. Tasmanian Institute of Agricultural Research. http:// www.horticulture.com.au. [1 September 2009].

Hikmia Z, Supramana, Suastika G. 2012. Identifikasi species Meloidogyne spp. penyebab umbi bercabang pada tanaman wortel di Jawa Timur. Jurnal Fitopatologi Indonesia. 8(3): 73-78.

Kurniawan W. 2010. Identifikasi penyakit umbi bercabang pada wortel, Daucus carota (L.) di Indonesia. [Thesis]. Bogor (ID): Institut Pertanian Bogor.

Luc M, Sikora RA, Bridge J. 2005. Plant Parasitic Nematodes in Subtropical and Tropical Agricultural. Ed ke-2. USA: CABI Publishing.

Meng QP, Long H, Xu JH. 2004. PCR assays for rapid and sensitive identification of three major root-knot nematodes, Meloidogyne incognita, M. javanica, and M. arenaria. Acta Phytopathologica Sinica. 34: 204-210.

Orui Y. 1998. Identification of Japanese species of the genus Meloidogyne (Nematoda: Meloidogynidae) by PCR-RFLP analysis. Appl. Entomol. Zool. 33: 43-51.

Shurtleff MC, Averre CW. 2005. Diagnosing Plant Diseases Caused by Nematodes. USA: APS Press Minnesota.

Sorensen E, Long G. 2000. Carrot IPM Survey Results http://ipm.wsu.edu/ Surveys/Carrot.html. [2 September 2009].

Sorensen EJ. 2002. Crop Profile for Carrots in Washington. Washington State University. http:// caheinfo.wsu.edu. [2 September 2009].

Taher M, Supramana, dan Suastika G. 2012. Identifikasi Meloidogyne penyebab penyakit umbi bercabang pada wortel di Dataran Tinggi Dieng. Jurnal Fitopatologi Indonesia. 8(1): 16-21.

Tanaka JS, Nakagawa Y, Sakuoka R. 1997. Carrots. College of Tropical Agriculture and Human Resources University of Hawai.

Taylor AL, Sasser JN, Nelson LA. 1982. Relationship of climate and soil characteristic to geographical distribution of Meloidogyne species in agricultural soils. Coop. Public. Dept. Plant Pathol. NC State Univ. NC State Graphics.

Vrain TC. 1982. Relationship between Meloidogyne havla density and damage to carrots in organic soil. J nematol. 14: 50-57.

Wishart J, Phillips MS, Blok VC. 2002. Ribosomal intergenic spacer: a polymerase chain reaction diagnostic for Meloidogyne chitwoodi, M. fallax and M. hapla. Phytopathology 93: 884-892.

Zijlstra C, Lever AEM, Uenk BJ, Van Silfhout CH. 1995. Differences between ITS regions of isolated of root-knot nematodes Meloidogyne hapla and M. chitwoodi. Phytopathology: 85: 1231-1237.

Published
2014-08-23
How to Cite
Supramana., & SuastikaG. (2014). Spesies Nematoda Puru Akar (Meloidogyne spp.) yang Berasosiasi dengan Penyakit Umbi Bercabang pada Wortel: Penyakit Baru di Indonesia. Jurnal Ilmu Pertanian Indonesia, 17(2), 108-112. Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/8324