EVALUASI KEMURNIAN GENETIK DENGAN MARKA MIKROSATELIT DAN APLIKASI RIZOBAKTERIA UNTUK MENINGKATKAN PRODUKSI DAN MUTU BENIH JAGUNG HIBRIDA

  • Memen Surahman
  • . Giyanto
  • Andi Takdir
  • Awaludin Hipi
Keywords: Genetic purity, physiologycal quality, rhizobacteria, phosphate efficiency, seed of maize hybrid

Abstract

One effort to improve of high-quality of maize seed were the development and application of methods for genetic quality testing, such as SSR marker. Another effort was used of rhizobacteria for increased the availability of nutrients, especially P in the soil. The aim of this study were: 1) Microsatellite markers specific to male and female parents of maize hybrid; 2) Seed genetic purity by using molecular marker; 3) Rhizobacteria that could increase the physiological quality of maize hybrid seed; and 4) Rhizobacteria that could increase the growth of plant and efficiency of fertilizer P. The result showed that: 1) From five markers tested, three markers namely phi96100, phi328175 and phi072 produced polymorphic bands and capable to distinguish parental lines of two maize hybrids. Microsatellite marker phi96100 was specific used for testing genetic purity of cv.Bima-4 and phi072 for cv.Bima-3. While phi328175 was specific markers to both hybrids maize. The test of cv. Bima-3 and Bima-4 indicated that genetic purity of both varieties were 97.5% and 80% respectivelly; 2) Isolates B28 and B46 could increase IV 19% and 22% respectively, and had a high speed of growth compared controls; 3). Isolates B46, B42, B13, P14, P31, AB2, AB3, AB11, ATS4, and ATS5 could increased of germination compared to control; 4) Treatment of rhizobacteri significantly affects on plant height 2, 4 and 6 week after planting (WAP); 5) P fertilizer dosage were not significantly influenced on the number of leaves at 2 and 4 WAP, but significant at 6 WAP. Isolate of B28, B42 and ATS4 were potential for increased of plant growth. 

Downloads

Download data is not yet available.

Author Biographies

Memen Surahman
Departemen Agronomi dan Holtikultura, Fakultas Pertanian, Institut Pertanian Bogor
. Giyanto
Departemen Proteksi Tanaman Jl. Kamper Kampus IPB Bogor
Andi Takdir
Balai Penelitian Serealia, Jl. Dr. Ratulangi 274 Maros 90514
Awaludin Hipi
Departemen Agronomi dan Holtikultura, Fakultas Pertanian, Institut Pertanian Bogor

References

Afzal I., Shahzad M.A. Basra, N. Ahmad, M.A. Cheema, E.A. Warraich, A. Khaliq. 2002. Effect of Priming and Growth Regulator Treatments on Emergence and Seedling Growth of Hybrid Maize (Zea mays L.). Int. J. Agri. Biol. 4 (2): 303-306.

Ahmad F, Ahmad I, Khan M.S. 2005. Indoleacetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonad in the presence and absence of tryptophan. Turk J. Biol 29:29-34.

Agrios G.N. 2005. Plant Pathology. 5th ed. London: Elsevier Academic Press Publications.

Badan Litbang Pertanian. 2008. Panduan Umum Pengelolaan Tanaman Terpadu Jagung. Jakarta.

Baihaki, A. 2004. Mengantisipasi persaingan dalam menuju swasembada varietas unggul. Makalah Simposium PERIPI di Balitro Bogor, 5-7 Agustus 2004. 17 halaman.

Baset Mia M.A., Z.H. Shamsuddin, Z. Wahab, M.Marziah. 2010. Effect of plant growth promoting rhizobacterial (PGPR) inoculation on growth and nitrogen incorporation of tissue-cultured Musa plantlets under nitrogen-free hydroponics condition. Australian J. Crop Sci. 4(2): 85-90.

Bennett, M.A., Jr. L. Waters. 1987. Seed hydration treatments for improved sweet maize germination and stand establishment. J. Amer.Soc. Hort. Sci., 112: 45–9.

BPS Indonesia. 2010. Statistik Pertanian Indonesia. Badan Pusat Statistik dan Direktorat Jendral Tanaman Pangan. Jakarta

Business News. 2010. Produksi jagung tahun 2010 tidak mencapai target. Jakarta, 20 November 2010. Download tanggal 27 Januari 2011.

Couch SR, SD. Tanskley. 1991. Development and use of restriction fragment length polymorphism in rice breeding and genetic. In Khush GS. And Toenniessen GH. (eds) Rice Biotechnology. IRRI. Los Banos. Philipines. p.109-133.

Desai S et al. 2007. Seed inoculation with Bacillus spp. improves seedling vigour in oil-seed plant Jatropha curcas L. Biol. Fertil. Soil 44: 229-234.

Egamberdiyeva D et al. 2006. Improvement of wheat and cotton growth and nutrient uptake by phosphate solubilizing bacteria. 26th Southern Conservation. Tillage Converence.

Egamberdiyeva D. 2008. Plant growth promoting properties of rhizobacteria isolated from wheat and pea grown in loamy sand soil. Turkish J. Biol 32: 9-15.

George, M.L.C., E. Regalado, M. Warburton, S. Vasal, D. Hoisington. 2004. Genetic diversity of maize inbred lines in relation to downy mildew. Euphytica 135: 145-155.

Gholami.A., S. Shahsavani, S. Nezarat. 2009. The Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Germination, Seedling Growth and Yield of Maize. World Academy of Science, Engineering and Technology 49 (2009) : 19-24.

Glick B.R., Zhenyu Cheng, J Czarny, Jin Duan. 2007. Promotion of plant growth by ACC deaminase producing soil bacteria. J. Plant Pathol 119 : 329-339.

Glickman E, Dessaux Y. 1995. A critical examination of specificity of slakowski reagent for indolic compound produced by phytopathogenic bacteria. App Environ Microbiol 61: 793-796.

Goenadi DH. 2008. Pupuk dan teknologi pemupukan berbasis hayati. Jakarta: Yayasan John Hi-Tech Idetama.

Gupta, PK., HS Balyan, PC Sharma, B Ramesh. 1996. Microsatellites in plants:A new class of molecular markers. Journal of Current Sci. 7 (1): 45-54

Harris D, RS Tripathi, A Joshi. 2000. Onfarm priming to improve crop establishment and yield in direct seeded rice in IRRI : International Workshop on Dry –seeded Rice Technology. held in Bangkok, 25-28 January 2000. The international Rice Research institute. Manila. The Philippines. 164p.

Jone, US. 1982. Fertilizers and soil fertility. 2nd ed. Reston Publ.Co.Reston. Virginia

Karama. S. 2004. Posisi perbenihan Indonesia sekarang dan antiisipasi terhadap benih impor. Makalah Simposium PERIPI di Balitro Bogor, 5-7 Agustus 2004.

Kasryno F, E Pasandaran, Suyamto, MO Adnyana. 2007. Gambaran umum ekonomi jagung Indonesia. dalam Jagung. Teknik produksi dan pengembangan. Badan Penelitian dan Pengembangan Pertanian. Pusat Penelitian dan Pengembangan Pertanian Tanaman Pangan. Hal. 474-497.

Khalimi, Kh., dan G.N.A.S. Wirya. 2009. Pemanfaatan plant growth promoting rhizobacteria untuk biostimulants dan bioprotectants. J. Ecotrophic 4 (2) : 131‐135.

Kloepper JW et al. 1999. Plant root bacterial interaction in biological control of soilborne disease and potential extension to systemic and foliar disease. Austral Plant Pathol 28: 21-26.

Liu Li-Wang, Yan Wang, Yi-Qin Gong, Tong-Min Zhao, Guang Liu, Xiao-Yan Li, Fa-Min Yu. 2007. Assesment of genetic purity of tomato (Lycopersicum esculentum L.) hybrid using molekuler markers. Journal of Scientia Horticulturae 115 (2007): 7-12. www.elsevier .com.

Macaulay M, L Ramsay, W Powell, R Waugh. 2001. A representative, highlyinformative, ‘genotyping set’ of barley SSR. Theor. Appl. Genet. 102: 801-809.

Pabendon MB, MJ Mejaya, Subandi, M Dahlan. Sidik jari empat varietas jagung hibrida beserta tetuanya berdasarkan marka mikrosatelit. Zuriat 2005; 16 (2): 192-200.

Prihartini T. 2009. Mikroorganisme meningkatkan efisiensi pemupukan fosfat. www.pustakadeptan.go.id/publikasi/wi.303.kdpdf.

Subba-Rao, NS. 2007. Mikroorganisme dan Pertumbuhan Tanaman. UI Press. Jakarta.

Rodríguez H, R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Department of Microbiology, Cuban Research Institute on Sugarcane By-Products (ICIDCA), Havana, Cuba. http://www.molecular-plant-biotechnology.

Saenong, S. Zubactirodin, Y. Sinuseng, Rahmawati, A. Hipi. 2005. Peluang pengembangan perbenihan berbasis komunal di pedesaan Nusa Tenggara Barat. Pros.Sem.Nas. Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian. Bogor. Mataram Agustus 2005.

Senior, M.L., J.P. Murphy, M.M. Goodman, and C.W. Stuber. 1998. Utility of SSR for determining genetic similarities and relationships in maize using an agarose gel system. J. Crop Sci. 38: 1088-1098.

Silva HAS et al. 2004. Rhizobacterial Induction of systemic resistance in tomato plants: non-spesific protection and increace in enzyme activities. Bio Control 29:288-295.

Sundstrom, F.J., R.B. Reader, R.L. Edwards, 1987. Effect of seed treatment and planting method on Tabasco pepper. J. Amer. Soc.Hort. Sci. 112: 641-4.

Widayanti T. 2006. Isolasi dan karakterisasi Bacillus sp. indigenus penghasil asam indol asetat asal tanah rizosfer [Skripsi]. Dept.Biologi. FMIPA. IPB. Bogor.

Yusepi TT. 2011. Kemampuan aktinomiset endofit dalam meningkatkan pertumbuhan tanaman padi (Oryza sativa L.) melalui aktivitas asam Indol Asetat [Skripsi]. Bogor: Departemen Biologi. Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.

Published
2012-05-05
How to Cite
SurahmanM., Giyanto., TakdirA., & HipiA. (2012). EVALUASI KEMURNIAN GENETIK DENGAN MARKA MIKROSATELIT DAN APLIKASI RIZOBAKTERIA UNTUK MENINGKATKAN PRODUKSI DAN MUTU BENIH JAGUNG HIBRIDA. Jurnal Ilmu Pertanian Indonesia, 17(1), 22-34. Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/8034