Plant Breeding Techniques in Tissue Culture to Improve the Quality of Orchids

  • Mukhamad Su'udi Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • Waki’atil Rosida Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • El Shania Ravitamala Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • Arif Mohammad Siddiq Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • Rendy Setiawan Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • Dwi Setyati Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • Asyifa Yasmin Ningrum Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121
  • Wasiatur Roziqoh Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jl. Kalimantan Tegalboto No.37, Krajan Timur, Sumbersari, Kec. Sumbersari, Kabupaten Jember, Jawa Timur 68121

Abstract

Conventional orchid cultivation is thought to be ineffective and time-consuming. Simple tissue culture procedures are insufficient to increase orchid quality. The need for ongoing development can be met by integrating different plant breeding strategies in orchid tissue culture, such as induction mutation, elicitor addition, and genetic transformation. The purpose of this article was to report on current improvements in the use of plant breeding techniques to orchid tissue culture to increase orchid quality. This article referenced significant scientific publications. Orchid tissue culture using mutation induction is used to develop improved variations. Giving elicitors can cause orchid plants to conserve themselves and become more resistant to diseases. The required transgene can be introduced into the genome of cultivated orchids via Agrobacterium. These advancements have the potential to revolutionize orchid cultivation.

Keywords: biotechnology, orchid, tissue culture

Downloads

Download data is not yet available.

References

Amanda MD, Dewanti VP, Okviandari P, Sugiharto B. 2023. Induksi mutasi secara in vitro anggrek Dendrobium gabriella suryajaya melalui pemberian kolkisin. Agrium. 26(1): 21–28. https://doi.org/10.30596/agrium.v26i1.13819

Bani R, Dewanti P, Restanto DP, Widuri LI, Alfian FN. 2022. Pengaruh pemberian kitosan pada tahap aklimatisasi anggrek Dendrobium Sonia. Jurnal Penelitian Pertanian Terapan. 22(2): 146–154. https://doi.org/10.25181/jppt.v22i2.2264

Bhattacharyya S, Banerjee N. 2020. Influence of cytokinins on rhizome mediated growth and morphogenesis of an endangered medicinal orchid Geodorum densiflorum (Lam.) Schltr. Plant Tissue Culture and Biotechnology. 30(1): 65–75. https://doi.org/10.3329/ptcb.v30i1.47792

Bhattacharyya P, Suman K, Pramod T. 2016. High frequency regeneration protocol for Dendrobium nobile: A model tissue culture approach for propagation of medicinally important orchid species. South African Journal of Botany. 104: 232–243. https://doi.org/10.5455/jabet.2020.d128

Bhowmik TK. Rahman MM. 2017.Micropropagation of commercially important orchid Dendrobium palpebrae Lindl. through in vitro developed pseudobulb culture. Journal Advances Biotechnol. Experimental Therapeutics. 3(3): 225–232. https://doi.org/10.5455/jabet.2020.d128

Cardoso JC, Zanello, Chen CA. 2020. An overview of orchid protocorm-like bodies: Mass propagation, biotechnology, molecular aspects, and breeding. International Journal of Molecular Sciences. 21(3): 985. https://doi.org/10.3390/ijms21030985

Chand K, Shah S, Pant B. 2023. Growth promoting effect of endophytic bacteria bacillus subtilis from leaves of vanda cristata and its potential impact on in vitro growth of orchid. Journal of Nepal Biotechnology Association. 4(1): 8–16. https://doi.org/10.3126/jnba.v4i1.53441

Chen B, Stephen JT, Jianmin L, Qianzhen L, Huihua F, Juan Z. 2014. Micropropagation of the endangered medicinal orchid, Dendrobium officinale. Life Science Journal. 1(9): 526–530.

Cui HY, Hosakatte NM, Sang HM, Yong-Yi C, Kee-Yoeup P. 2015. Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds. Horticulture, Environment, and Biotechnology. 56: 114–122. https://doi.org/10.1007/s13580-015-0082-5

Handini E, Sukma D, Sudarsono, Roostika I. 2020. Radiosensitivitas dan pertumbuhan in vitro protocorm anggrek ‘tien soeharto’ (Cymbidium hartinahianum J.B. Comber & R.E. Nasution). Journal Agronomi Indonesia. 48(3): 323–330. https://doi.org/10.24831/jai.v48i3.29170

Hardjo PH, Binarto CWS, Savitri WD. 2016. Induksi protocorm-like bodies (Plbs) Vanda tricolor Lindl. var. Pallida. Proceeding Seminar Nasional Biodiversitas VI: 172–179.

Hsieh KT,Su L, Aku WW, Liang JC. 2020. Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene. Botanical Studies. 61(10): 93–109. https://doi.org/10.1186/s40529-020-00288-0

Irsyadi MB, Shania NF, Alya FA, Jennifer G, Widhi DS, Aziz P. 2022. Transformasi genetik in planta: metode cepat memperoleh produk rekayasa genetika. Prosiding Seminar Nasional Penelitian dan Pengabdian Masyarakat. 7(1): 243–252

Kang H, Kang KW, Kim DH, Sivanesan I. 2020. In vitro propagation of Gastrochilus matsuran (Makino) Schltr., an endangered epiphytic orchid. Plants. 9(4): 1–10. https://doi.org/10.3390/plants9040524

Khatun K, Ujjal KN, Md. Shafikur R. 2020. Tissue culture of Phalaenopsis: present status and future prospects. Journal Advances Biotechnol Experimental Therapeutics. 3(3): 273–285. https://doi.org/10.5455/jabet.2020.d135

Khumkarjorn N, Sudarat T, Mamoru Y, Preekamol, Pornthap T. 2017. Agrobacterium-mediated transformation of Dendrobium orchid with the flavanone 3-hydroxylase gene. Turkish Journal of Botany. 41: 442–454. https://doi.org/10.3906/bot-1701-13

Kurniadi SK., Irawati F, Putra SED, Hardjo PH. 2023. Induction of protocorm-like bodies (plbs) Phalaenopsis spp. hybrids mutation through ultraviolet irradiation (uv254) and ethyl methane sulfonate (EMS). Agriprima Journal of Applied Agricultural Sciences. 7(1): 1–15. https://doi.org/10.25047/agriprima.v7i1.512

Lal N, Singh M. 2020. Prospects of plant tissue culture in orchid propagation: a review. Indian Jurnal of Biology. 7(2): 103–110.

Lawrie MD Z. Layina D, Ningtias R, Alifianto FN, Indrianto A, Purwantoro A. Semiarti E. 2021. In vitro germination and flowering of Dendrobium capra J.J. Smith, An endemic orchid of java. Hayati. Journal of Biosciences. 28(2): 172–180. https://doi.org/10.4308/hjb.28.2.172

Li C, Dong N, Zhao Y, Wu S, Liu Z, Zhai J. 2021. A review for the breeding of orchids: Current achievements and prospects. Horticultural Plant Journal. 7(5): 380–392. https://doi.org/10.1016/j.hpj.2021.02.006

Lisnandar DS, Mudyantini W, Pitoyo P. 2012. Pengaruh pemberian variasi konsentrasi NAA (α-naphthaleneacetic acid) dan 2.4-D terhadap induksi protocorm like bodies (PLB) anggrek macan (Grammatophyllum scriptum (Lindl.). Bioteknologi. 9(2): 6672. https://doi.org/10.13057/biotek/c090205

Lun LP, Tsung CJ. 2014. Plant regeneration via callus culture and subsequent in vitroflowering of Dendrobium huoshanense. Acta Physiology Plant. 36: 2619–2625. https://doi.org/10.1007/s11738-014-1632-7

Maharjan S, Thakuri LS, Thapa BB, Pradhan S, Pant KK, Joshi GP, PantB. 2020. In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture. Nepal Journal of Science and Technology. 19(1): 39–47. https://doi.org/10.3126/njst.v19i1.29737

Muna S, Beura S, Patra SK. 2016. Standardization of media mixture for hardening of in vitro plantlets of Dendrobium cv. Sonia-17. Agriculture Science Digest. 36(1): 78–80.

Mursyanti E. Aziz P, Sukarti M, Endang S. 2015. Induction of somatic embryogenesis through overexpression of atrkd4 genes in Phalaenopsis “Sogo Vivien”. International Journal of Biotechnology. 20(1): 42–53. https://doi.org/10.22146/ijbiotech.15276

Ningsih IY. 2014. Pengaruh elisitor biotik dan abiotik pada produksi flavonoid melalui kultur jaringan tanaman. Pharmacy. 11(2): 1–16.

Pant B, Thapa D. 2016. In vitro mass propagation of an epiphytic orchid, Dendrobium primulinum Lindl. through shoot tip culture. African Journal of Biotechnology. 11(42): 9970–9974.

Parthibhan S, Rao MV, Kumar. 2015. In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid.Journal of Genetic Engineering and Biotechnology. 13(2): 227–233. https://doi.org/10.1016/j.jgeb.2015.07.001

Paul S, Kumaria S, Tandon P.2012. An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India. AoB Plants. vol(issue): 1–7. https://doi.org/10.1093/aobpla/plr032

Pujasatria G, Miura C, Kaminaka K. 2020. In vitro symbiotic germination: a revitalized heuristic approach for orchid species conservation. Plants. 9: 1–15. https://doi.org/10.3390/plants9121742

Putri M.S, Yuliani HE, Kurniawati S, Putri WR, Amin HN, Rahayu P. 2023. Literature review: Pembelajaran kultur jaringan untuk menumbuhkan science motivation. Prosiding Webinar Biofair. 23–34.

Rojanawong T, Thepsithar C, Thongpukdee A. 2006. Micropropagation of Phalaenopsis Cygnus ‘silky moon’ from leaf segments. Proceedings of the 32nd Congress on Science and Technology of Thailand.

Romeida A, Supanjani, Sinaga SS. 2018. Low-Cost Media for in vitro multiplication and development of protocorm like bodies (PLBs) of Eulophia graminea Orchid. International Journal on Advanced Science Engineering Information Technology. 8(1): 78–84. https://doi.org/10.18517/ijaseit.8.1.1162

Romeida A, Sutjahjo SH, Purwito A, Sukma D, Rustikawati. 2012. Induksi mutasi protocorm like body (plb) anggrek spathoglottis plicata blume. aksesi bengkulu pada sebelas taraf dosis iradiasi sinar gamma. Prosiding Simposium dan Seminar Bersama Peragi-Perhorti-Peripi-Higi. 381–387.

Semiarti E, Ixora SM, Agus S, Bekti S, Bestari IAP , Soenghoe J, Machida Y, Machida C. 2015. Induction of in vitro flowering of indonesian wild orchid, Phalaenopsis amabilis (L.) Blume. The 3rd International Conference on Biological Science. 2: 398–404. https://doi.org/10.18502/kls.v2i1.182

Semiarti EA, Purwontoro, Mercuriani IS, Sasongko AB, Slamet A, Utami BS. 2014. Regulasi genetik inisiasi pembungaan pada tanahan anggrek Phalaenopsis amabilis (L.) blume pada kultur in vitro. Yogyakarta (ID): Seminar Nasional Bioteknologi. Universitas Gajah Mada. 1–9.

Setiawan RB, Nurul K, Diny D. 2015. Induksi mutasi kalus embriogenik gandum (Triticum aestivum L.) melalui iradiasi sinar gamma untuk toleransi suhu tinggi. Jurnal Agronomi Indonesia. 43(1): 36–44. https://doi.org/10.24831/jai.v43i1.9589

Shah S, Shah B, Sharma R, Rekadwad B, Shouche YS, Sharma J, Pant JB. 2022. Colonization with non-mycorrhizal culturable endophytic fungi enhances orchid growth and indole acetic acid production. BMC Microbiology. 22(101): 1–13. https://doi.org/10.1186/s12866-022-02507-z

Silva L, Karla LA, Cristina S, Alejandro M, Rodrigo A, Magno P, Valter F, Francisco C. 2015. In vivo antimalarial activity and mechanisms of action of 4- nerolidylcatechol derivatives. Antimicrobial Agents and Chemotherapy. 59(6): 78–89. https://doi.org/10.1128/AAC.05012-14

Solichatun S, Pitoyo A, Etikawati N, Herawati E, Ardo T. 2020. Penerapan teknologi kultur jaringan bagi petani anggrek di Desa Berjo, Karanganyar. Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR). 3: 217223. https://doi.org/10.37695/pkmcsr.v3i0.935

Sornchai P, Rakchanok K, Parichart B, Sontichai C, Wachiraya I, Sermsiri C. 2015. Genetic transformation of Dendrobium 'Sonia Earsakul' with antisense Carica papaya ACO1 gene. Modern Applied Science. 9(12): 125–133. https://doi.org/10.5539/mas.v9n12p125

Suetsugu K. 2020. A novel seed dispersal mode of Apostasia nipponica could provide some clues to the early evolution of the seed dispersal system in Orchidaceae. Evolution Letters. 4: 457–464. https://doi.org/10.1002/evl3.188

Syahfitri D, Nurcahyani E, Chrisnawati L, Ernawiati E. 2022. Analisis kandungan klorofil dan indeks stomata planlet anggrek Dendrobium hasil induksi asam salisilat secara in vitro. Analit: Analytical and Environmental Chemistry. 7(2): 165–176. https://doi.org/10.23960/aec.v7i02.2022.p165-176

Utami ESW, Sucipto H,Yosephine SWM. 2018. Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: an important medicinal orchid. Journal of Genetic Engineering and Biotechnology. 16(2): 703–709. https://doi.org/10.1016/j.jgeb.2018.02.002

Vargas, L, Neftali O. 2018. Somatic Embryogenesis: Fundamental Aspects and Applications. Switzerland (SW): Springer.

Wang ZH, Tu HY, Ye QS. 2006. Rapid propagation and in vitro flowering of Dendrobium moniliforme (L.) Sw. Plant Physiology Communication. 42: 1143–1144.

Yan Y, Zhu X, Yu Y, Li C, Zhang Z, Wang F. 2022. Nanotechnology strategies for plant genetic engineering. Advanced Material. 34: 2106945. https://doi.org/10.1002/adma.202106945

Yasmin ZF, Aisyah S I, Sukma D. 2018. Pembibitan (kultur jaringan hingga pembesaran) anggrek Phalaenopsis di hasanudin orchids, Jawa Timur. Buletin Agrohorti. 6(3): 430–439. https://doi.org/10.29244/agrob.v6i3.21113

Yong HP, Kyung WK, Doo HK,Iyyakkannu S. 2018. In vitro propagation of Cymbidium goeringii Reichenbach fil. through direct adventitious shoot regeneration. Physiology Molecular Biology Plants. 24(2): 307–313. https://doi.org/10.1007/s12298-017-0503-2

Zhao DK, Li CF, Chen ZY, Yang JB. 2012. In vitro flowering and conservation of Dendrobidium strongylanthum Rchb.f. Subtropical Plant Science. 41: 48–50.

Zulwanis, Nintya S, Jose G, Endang S. 2020. The expression of AtRKD4 transgene during induction of somatic embryogenesis in transgenic Dendrobium phalaenopsis orchid carrying 35S::GR::AtRKD4. AIP Conference Proceeding. 2260: 1–7. https://doi.org/10.1063/5.0015873

Published
2024-11-19
How to Cite
Mukhamad Su’udi, RosidaW., RavitamalaE. S., SiddiqA. M., SetiawanR., SetyatiD., NingrumA. Y., & RoziqohW. (2024). Plant Breeding Techniques in Tissue Culture to Improve the Quality of Orchids. Jurnal Ilmu Pertanian Indonesia, 30(1), 116-122. https://doi.org/10.18343/jipi.30.1.116