Characterization of the 4th Exon of DRD4 Gene in Arab Tiara G15 Chicken Breed with Feather Pecking Behaviors

  • Achmad Farajallah Department of Biology, IPB University, Bogor
  • Pristina Tuti Wijayanti Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University, Jl. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java, Indonesia, 16680
  • Achmad Alfiyan Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University, Jl. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java, Indonesia, 16680
  • Maria Ulfah Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, IPB University, Jl. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java, Indonesia, 16680

Abstract

Feather Pecking (FP) is a behavior which founded in chicken which may cause feather damage and death. Feather pecking behavior were influenced by various factor, one of them is genetic factors. One of the gene that was affecting this behavior is the Dopamine Receptor D4 (DRD4) gene. This study was aimed to characterize the 4th exon of DRD4 gene in Arab chicken golden red TIARA G15. The TIARA G15 chicken sample used in this study consisted of 30 individuals which were divided into 3 groups, namely the high feather pecking (HFP) (n=10), low feather pecking (LFP) (n=10) and control (n=10) groups. Blood sample of the chicken samples was used as genetic material source which its total genome were extracted using commercial DNA extraction kit. The 4th exon of DRD4 gene were amplified using PCR method with primer pair MU140-141. The amplicon of the individual of each group was pooled together in 1 reaction tube and then were sequenced using Sanger methods. The length of the nucleotide sequence from each group of chickens that was obtained were 642 bp long, with the 3rd intron fragment were 55 bp, the 4th exon were 202 bp and 3'UTR fragment were 385 bp. Based on nucleotide variation analysis, in the three groups of TIARA G15 golden red Arabic chickens, no nucleotide variations were found in either the 3rd intron, the 4th exon and 3'UTR regions of the DRD4 gene.

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Published
2024-03-18
How to Cite
FarajallahA., WijayantiP. T., AlfiyanA., & UlfahM. (2024). Characterization of the 4th Exon of DRD4 Gene in Arab Tiara G15 Chicken Breed with Feather Pecking Behaviors. Jurnal Ilmu Pertanian Indonesia, (00). Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/50011
Issue
Vol. 0 No. 00 (2024): inpress
Section
Articles

Copyright (c) 2024 Achmad Farajallah, Pristina Tuti Wijayanti, Achmad Alfiyan, Maria Ulfah

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