Histopatologi Hepar Tikus Dengan Induksi Pakan Tinggi Lemak Setelah Pemberian Biji Mahoni
Abstract
High accumulation of lipids in hepatocytes can trigger an increase in the production of radical compounds, leading to fatty liver disease. This condition induces histological changes in the liver, especially in hepatocytes, and affects the surrounding structures. Mahogany seeds have the potential to act as a hypolipidemic agent in treating fatty liver. Mahogany seeds are known to contain phytochemical compounds which act as antioxidants. This study aimed to determine liver histopathology of rats by inducing high-fat diet after giving mahogany seeds ethanol extract which was observed through the percentage of steatosis, hepatocyte diameter, and hepatosomatic index. This study involved thirty male Sprague Dawley rats, divided into 6 groups: P0 (given commercial feed), P1 (given high-fat diet), P2 (given high-fat diet and simvastatin at a dose of 8 mg/200 g BW), P3, P4, and P5 (given high-fat diet and mahogany seeds ethanol extract at a dose of 14, 28, and 56 mg/200 g BW). The results showed that the hepatosomatic index among all treatment groups did not differ significantly; however, the percentage of steatosis and hepatocyte diameter in the groups given mahogany seeds ethanol extract significantly differed from the group given only high-fat feed. Based on these results, it can be concluded that mahogany seed ethanol extract can maintain the histological structure of the liver in male Sprague Dawley rats with fatty liver.
Keywords: hepatocyte, hepatosomatic index, high-fat diet, mahogany seeds, steatosis
Downloads
References
Arini W, Isdadiyanto S, Sitasiwi AJ. 2020. Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss.) terhadap Struktur Ren Tikus Putih (Rattus norvegicus L.) yang Diberi Pakan Tinggi Lemak. Buletin Anatomi dan Fisiologi. 5(2): 157–165. https://doi.org/10.14710/baf.5.2.2020.157-165
Astuti A, Antriana N, Zelpia. 2017. Biji Mahoni (Swietenia mahogani) Menurunkan Glukosa Darah Pada Diabetes Melitus Tipe II. Jurnal IPTEKS Terapan. 11(3): 187–193. https://doi.org/10.22216/jit.2017.v11i3.1964
Bedossa P. 2016. Histological Assessment of NAFLD. Digestive Diseases and Sciences. 61(5): 1348–1355. https://doi.org/10.1007/s10620-016-4062-0
Başaranoglu M, Ormeci N. 2014. Nonalcoholic fatty liver disease: diagnosis, pathogenesis, and management. Turk J Gastroenterol. 25(2): 127–132. https://doi.org/10.5152/tjg.2014.7675
Cao D, Feng F, Xiong C, Li J, Xue H, Zhao Y, Wang Y, Tu Y, Zhao Y. 2021. Changes in lipid properties of duck egg yolks under extreme processing conditions. Poultry Science. 100(7): 1–12. https://doi.org/10.1016/j.psj.2021.101140
Ekananda N. 2015. Bay Leaf in Dyslipidemia Therapy. Medical Journal of Lampung University. 4(4): 64–69.
Entredicho AAB, Harina GVD, Quimio EE, Sanchez PJR, Quinto LF. 2019. Hypolipidemic Activity of Ethanolic Extract of Philippine Mahogany Seed (Swietenia macrophylla). LPU-St. Cabrini Journal of Allied Medicine. 3(2): 46–51.
Fan L, Qu X, Yi T, Peng Y, Jiang M, Miao J, Xiao P. 2020. Metabolomics of the protective effect of Ampelopsis grossedentata and its major active compound dihydromyricetin on the liver of high-fat diet hamster. Evidence-Based Complementary and Alternative Medicine. 2020(3472578): 1–15. https://doi.org/10.1155/2020/3472578
Fitriani RN, Sitasiwi AJ, Isdadiyanto S. 2020. Struktur Hepar dan Rasio Bobot Hepar Terhadap Bobot Tubuh Mencit (Mus Musculus L.) Jantan Setelah Pemberian Ekstrak Etanol Daun Mimba (Azadirachta Indica A. Juss). Buletin Anatomi dan Fisiologi. 5(1): 75–83. https://doi.org/10.14710/baf.5.1.2020.75-83
Ganesan P, Kaewmanee T, Benjakul S, Baharin BS. 2014. Comparative study on the nutritional value of pidan and salted duck egg. Korean journal for food science of animal resources. 34(1): 1–6. https://doi.org/10.5851/kosfa.2014.34.1.1
Gupta N, Gupta DK, Sharma PK. 2017. Condition factor and organosomatic indices of parasitized Rattus rattus as indicators of host health. Journal of parasitic diseases. 41(1): 21–28. https://doi.org/10.1007/s12639-015-0744-3
Hanung A, Saktini F, Gumay AR. 2019. Pengaruh Frekuensi Penggorengan Minyak Jelantah Terhadap Diameter Dan Gambaran Histopatologi Lumen Aorta Tikus Wistar (Rattus Novergicus). Diponegoro Medical Journal. 8(1): 26–37.
Isdadiyanto S, Mardiati SM, Sitasiwi AJ. 2021. Kadar Apoprotein A dan Apoprotein B Serum Darah Tikus Putih (Rattus norvegicus L.) Yang Diinduksi Pakan Tinggi Lemak Setelah diberi Ekstrak Etanol Daun Mimba (Azadirachta indica). Buletin Anatomi dan Fisiologi. 6(1): 1–7. https://doi.org/10.14710/baf.6.1.2021.49-55
Isdadiyanto S, Pratiwi AR, Mardiati SM. 2022. Liver Histopathology of Rats Induced by High-Fat Feed After Giving Neem Leaf Ethanol Extract. Biosaintifika Journal of Biology & Biology Education. 14(2): 254–262. https://doi.org/10.15294/biosaintifika.v14i2.34383
Kusuma AB, Saraswati TR, & Sitasiwi AJ. 2019. Efek pemberian daun mimba (Azadirachta indica) terhadap diameter hepatosit tikus (Rattus norvegicus). Bioma: Berkala Ilmiah Biologi. 21(2): 106–113. https://doi.org/10.14710/bioma.21.2.106-113
Lee J, Homma T, Fujii J. 2017. Mice in the early stage of liver steatosis caused by a high fat diet are resistant to thioacetamide-induced hepatotoxicity and oxidative stress. Toxicology letters. 277: 92–103. https://doi.org/10.1016/j.toxlet.2017.06.005
Mansi I, Frei CR, Pugh MJ, Makris U, Mortensen EM. 2013. Statins and musculoskeletal conditions, arthropathies, and injuries. JAMA internal medicine. 173(14): 1318–1326. https://doi.org/10.1001/jamainternmed.2013.6184
Mazzolini G, Sowa JP, Atorrasagasti C, Kucukoglu O, Syn WK, Canbay A. 2020. Significance of simple steatosis: an update on the clinical and molecular evidence. Cells. 9(11): 2458. https://doi.org/10.3390/cells9112458
Muhartono M, Agung Y, Nindya TP, Tri NS, Oktafany OF. 2018. Minyak Jelantah Menyebabkan Kerusakan pada Arteri Koronaria, Miokardium, dan Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Sprague dawley. JK Unila. 2(2): 129–135.
Mundiri NA, Maulida M, Tejasari M, Furqaani AR, Ekowati RR. 2019. Pengaruh Fraksi Air Buah Lemon terhadap Gambaran Morfologi Jaringan Hati Mencit Tua yang Diberi Pakan Tinggi Lemak. Jurnal Integrasi Kesehatan & Sains. 1(1): 49–53. https://doi.org/10.29313/jiks.v1i1.4321
Nadiroh A, Hariani D. 2022. Efek Ekstrak Daun Pepaya Jepang terhadap Kadar Kolesterol, Morfometri, dan Histologi Hepar Mencit Hiperkolesterolemia. LenteraBio Berkala Ilmiah Biologi. 11(1): 101–112. https://doi.org/10.26740/lenterabio.v11n1.p101-112
Nassir F, Rector RS, Hammoud, GM, Ibdah JA. 2015. Pathogenesis and prevention of hepatic steatosis. Gastroenterology and Hepatology. 11(3): 167–175.
Nunes C, Silva A, Soares E, Ganias K. 2011. The use of hepatic and somatic indices and histological information to characterize the reproductive dynamics of Atlantic sardine Sardina pilchardus from the Portuguese coast. Marine and Coastal Fisheries. 3(1): 127–144. https://doi.org/10.1080/19425120.2011.556911
Rahayu IN, Kumala AR. 2021. Penurunan Kadar Mda Tikus Putih Jantan Dengan Pemberian Diet Tinggi Lemak Dan Ekstrak Biji Mahoni. Surabaya Biomedical Journal. 1(1): 38–50. https://doi.org/10.30649/sbj.v1i1.7
Rahayu S, Supiyani A, Darmansyah R, Amalia R. 2022. Uji Hepatotoksisitas Minyak Jelantah Yang Dipurifikasi Karbon Aktif Ampas Tebu Pada Tikus Putih Sprague Dawley (Rattus norvegicus). Bioma. 18(2): 83–90. https://doi.org/10.21009/Bioma18(2).5
Rahmiati BF, Jauhari MT, Ardian J, Aini Q. 2020. Pengaruh Frekuensi Penggorengan dan Penambahan Sari Mengkudu (Morinda citrifolia) terhadap Jumlah Asam Lemak Trans pada Minyak Jelantah. Nutriology Jurnal Pangan, Gizi, Kesehatan. 1(1): 18–25. https://doi.org/10.30812/nutriology.v1i1.732
Santi W, Eliya M, Anisa M. 2021. Pengaruh Pemberian Ekstrak Etanol 96% Biji Mahoni (Swietenia Mahagoni L.) dalam Menurunkan Kadar Glukosa Darah pada Mencit (Mus Musculus) yang Diinduksi Aloksan. Jurnal Kedokteran dan Kesehatan Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya. 8(1): 69–74. https://doi.org/10.32539/V8I1.12655
Santosa AP, Trimurtini I, Hasan K. 2018. Efek Anti Hiperlipidemik Ekstrak Etanol Daging Buah Semangka Merah (Citrullus Lanatus) Terhadap Kadar Low Density Lipoprotein Pada Tikus Jantan Galur Wistar (Rattus Norvegicus). JIMKI Jurnal Ilmiah Mahasiswa Kedokteran Indonesia. 6(2): 41–48.
Sari JI, Jusu AA, Krisnuhoni E. 2018. Perbandingan Berat Hati Tikus Spraque Dawley yang Diberikan Diet Kurang Kalori, Cukup Kalori, dan Tinggi Kalori pada Masa Pertumbuhan. Alami Journal (Alauddin Islamic Medical) Journal. 2(2): 15–19. https://doi.org/10.24252/alami.v2i2.12698
Sukardiman, Ervina M. 2020. The recent use of Swietenia mahagoni (L.) Jacq. as antidiabetes type 2 phytomedicine: A systematic review. Heliyon. 6(3): 1–8. https://doi.org/10.1016/j.heliyon.2020.e03536
Takapaha VJ, Simbala HE, Antasionasti I. 2022. Uji In Vivo Ekstrak Bawang Hutan (Eleutherine america Merr.) Terhadap Gambaran Makroskopis Organ Hati Tikus Putih Jantan Galur Wistar (Rattus norvegicus). Pharmacon. 11(1): 1335–1341.
Wahyuningtyas P, Sitasiwi AJ, Mardiati SM. 2018. Hepatosomatic Index (HSI) Dan Diameter Hepatosit Mencit (Mus musculus L.) Setelah Paparan Ekstrak Air Biji Pepaya (Carica papaya L.). Jurnal Akademika Biologi. 7(1): 8–17.
Xiao C, Dash S, Morgantini C, Hegele R A, Lewis GF. 2016. Pharmacological targeting of the atherogenic dyslipidemia complex: The next frontier in CVD prevention beyond lowering LDL cholesterol. Diabetes. 65(7): 1767–1778. https://doi.org/10.2337/db16-0046
Copyright (c) 2024 Agave Chintya Gabriella Sijabat, Sri Isdadiyanto, Agung Janika Sitasiwi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This journal is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License. Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. NonCommercial — You may not use the material for commercial purposes.
View JIPI StatsCounter
