Pengaruh Nanovaksin Berbasis Nanopartikel Hybrid Chitosan Terhadap Sel Darah Ikan Kerapu Cantang Yang Diinfeksi VNN

Gian Suryanatha Hartawan; Uun Yanuhar; Muhammad Musa; Heru  Suryanto; Gunanti  Mahasri; Apri Supii; Nico Rahman  Caesar

doi:10.18343/jipi.28.4.650

Gian Suryanatha Hartawan Program Magister Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Jl. Veteran No.16, Malang 65145
Uun Yanuhar Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Jl. Veteran No.16, Malang 65145
Muhammad Musa Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Jl. Veteran No.16, Malang 65145
Heru Suryanto Fakultas Teknik, Universitas Negeri Malang, Jl. Semarang No.5, Malang, 65145
Gunanti Mahasri Fakuiltas Perikanan dan Kelautan, Universitas Airlangga Kampus C, Jl. Dr.Ir. H. Soekarno, Surabaya 60115
Apri Supii Research Center of Marine and Land Bioindustry, National Research and Inovation Agency Indonesia, Malaka, Pemenang, Lombok Utara 83352
Nico Rahman Caesar Program Doktor Ilmu Lingkungan, Sekolah Pascasarjana, Universitas Brawijaya, Jl. MT. Haryono No.169, Malang 65145

DOI: https://doi.org/10.18343/jipi.28.4.650

Abstract

Cantang grouper (Epinephelus sp.) is a fish with a high economic value and has been widely cultivated. VNN disase, caused by Betanodavirus infection, can lead to mass mortality of cantang grouper. Precautions considered effective in preventing Viral nervous necrosis (VNN) disease include vaccination. This study aimed to determine the effect of administering recombinant Chlorella vulgaris protein nanovaccine based on chitosan hybrid nanoparticles in increasing the immune response of cantang grouper infected with VNN. The study consisted of five treatments: K+ (cantang grouper infected with VNN), K- (healthy cantang grouper), T1 (healthy cantang grouper given 33 µl of nanovaccine and then challenged with VNN), T2 (healthy cantang grouper given 66 µl of nanovaccine and then tested with a VNN challenge), and T3 (healthy cantang grouper given 112 µl of nanovaccine and then tested with a VNN challenge). The parameters observed were haematology, nanovaccine Relative percent survival (RPS), and Survival rate (SR) levels. The study results showed that administering the nanovaccine increased the immune response of the fish, as indicated by an increase in haematology response, RPS, and SR in cantang grouper compared to the treatment without nanovaccine administration. The best dose of nanovaccine in this study is 33 µl.

Keywords: fish disease, nanovaccines, virus, C. vulgaris, grouper

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