Isolasi dan Respons Tumbuh Cendawan Mutualistik Akar pada Beberapa Tanaman Pangan dan Kehutanan

Rida Oktorida  Khastini; Nampiah Sukarno; Utut Widyastuti  Suharsono†; Yasuyuki Hashidoko†

doi:10.18343/jipi.27.1.85

Authors

Rida Oktorida Khastini Program Studi Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680; Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Sultan Ageng Tirtayasa, Jl. Raya Palka No. KM 3, Panacangan, Cipocok Jaya, Serang 42124 ; Pusat Unggulan Inovasi Perguruan Tinggi Ketahanan Pangan-Inovasi Pangan Lokal, Universitas Sultan Ageng Tirtayasa, Jl. Raya Palka No. KM 3, Panacangan, Cipocok Jaya, Serang 42124
Nampiah Sukarno Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680; ; Pusat Penelitian Sumberdaya Hayati dan Bioteknologi (PPSHB), Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Utut Widyastuti Suharsono†
Yasuyuki Hashidoko† Fakultas Pertanian, Universitas Hokkaido, Jepang

DOI:

https://doi.org/10.18343/jipi.27.1.85

Abstract

The study aims to isolate and test the effectiveness of mutualistic root symbiont fungi isolates from the roots of rubber plants grown in marginal acidic soil plantations in increasing the growth of food crops and forestry plants. The fungal were isolated by root surface sterilization methods. We obtained 19 fungal isolates consisting of 8 genera, namely Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Penicillium Paecilomyces, Trichoderma, and mycelia sterilia. All isolates were subjected to a pathogenicity test on the Centrosema pubescens plant. Five out of the 19 fungal isolates increased plant growth and showed no disease symptoms, and the Aspergillus section Nigri FKK 3 isolate showed the best response. The isolate was further analyzed to assess the growth response of food crops (rice and corn) and forestry plants (Acacia auriculiformis and Paraserianthes falcataria). The treatments consisted of 3 phosphate (P) concentrations, namely 20%, 50%, and 100% of the recommended field applications. The combination of mutualistic fungal inoculation of Aspergillus section Nigri FKK 3 and 50% P concentration exhibited the highest biomass growth response compared to other treatments. This finding can provide basic information for developing fungal-based fertilizers to increase the productivity of food crops and forestry plants on sub-optimal land.

Keywords: food crops, phosphate fertilizer, forestry trees, plant growth improvement, root mutualistic fungi

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