Karakteristik Mikoriza Arbuskula Tanaman Serai Wangi (Cymbopogon nardus L.) di Lapangan Ternaungi dan Tidak Ternaungi

Nampiah Sukarno; Rahayu Laelandi; Ibnu  Qayim; Mega Putri Amelya

doi:10.18343/jipi.27.1.109

Nampiah Sukarno Departemen Biologi, Fakultas Matematika dan Ilmu Penetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Rahayu Laelandi Departemen Biologi, Fakultas Matematika dan Ilmu Penetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Ibnu Qayim Departemen Biologi, Fakultas Matematika dan Ilmu Penetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Mega Putri Amelya Departemen Biologi, Fakultas Matematika dan Ilmu Penetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

DOI: https://doi.org/10.18343/jipi.27.1.109

Abstract

Arbuscular mycorrhiza (AM) characteristics of citronella grass in the field have not been reported. This research aimed to study the AM characteristics of citronella grass grown in unshaded and shaded fields. The roots of citronella grass were collected from citronella grass plantations in Cianjur, West Java. The root samples were analyzed for AM structures, namely entry points, intercellular hyphae, arbuscules, and vesicles. The results showed that the citronella grass form AM colonization. The quality of root colonization differed between the two cultivation systems. The unshaded citronella grass had higher root colonization compared to shaded citronella grass. In the unshaded citronella grass, the number of arbuscules was 7 per cm of root length, whereas in the shaded citronella grass was 4 per cm of root length. The types of arbuscules observed were arum and intermediate. There were no differences in the number of entry points in the two cultivated systems, which was 3,5 entry points per cm of root length. The numbers of vesicles and internal hyphae in unshaded citronella grass were lower than that of in the shaded citronella grass. In the unshaded citronella grass, the number of vesicles and intracellular hyphae were 1,5 and 8,5 per cm root length, whereas in the shaded citronella grass were 3,5 and 11 per cm root length, respectively. Shading plants grown in the field were bamboo, banana, coffee, tea, and sugar palm. All the shading plants formed AM symbiosis with a colonization value of 7 to 30%. This research indicates that arbuscular mycorrhiza is an important component in the citronella grass cultivation in unshaded and shaded fields.

Keywords: Arbuscule, entry point, intercellular hyphae, root colonization, vesicle

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References

Barrett G, Campbell CD, Hodge A. 2014. The direct response of the external mycelium of arbuscular mycorrhizal fungi to temperature and the implications for nutrient transfer. Soil Biology and Biochemistry. 78: 109117. https://doi.org/10.1016/ j.soilbio.2014.07.025

Brundrett MC, Bougher N, Dell B, Grove T, Malajczuk N. 2008. Working with Mycorrhizas in Forestry and Agriculture. CSIRO Forestry and Forest Product. CSIRO Centre for Mediterranean Agricultural Research Wembley, WA. Bernie Dell. Mudorch University. Mudorch, WA.

Campanelli A, Ruta C, De Mastro G, Morone-Fortunato I. 2012. The role of arbuscular mycorrhizal fungi in alleviating salt stress in Medicago sativa L. var. icon. Symbiosis. 59(2), 6576. https://doi.org/ 10.1007/s13199-012-0191-1

Charles DJ. 2012. Lemongrass. In: Antioxidant Properties of Spices, Herbs and Other Sources. New York (USA). https://doi.org/10.1007/978-1-4614-4310-0_35

Cruz RMSD, Alberton O, Lorencete MDS, Cruz LSD, Gasparotto-Junior A, Cardozo-Filho L, Sauza SGHD. 2020. Phytochemistry of Cymbopogon citratus (D.C.) Stapf inoculated with arbuscular mycorrhizal fungi and plant growth promoting bacteria. Industrial Crops & Products. 149 (112340): 14. https://doi.org/10.1016/j.indcrop.2020.112340

Das P, Highland K. 2010. Arbuscular mycorrhizal fungi and dark septate endophyte colonization in bamboo from Northeast India. Frontiers of Agriculture in China. 4(3): 375382. https://doi.org/10.1007/ s11703-010-1013-y

Diagne N, Ngom M, Djighaly PI, Fall D, Hocher V, Svistoonoff S. 2020. Roles of Arbuscular Mycorrhizal Fungi on Plant Growth and Performance: Importance in Biotic and Abiotic Stressed Regulation. Diversity. 12(370): 125. https://doi.org/10.3390/d12100370

Genre A, Paola B. 2010. The making of symbiotic cells in arbuscular mycorrhizal roots. In: Koltai H., Kapulnik Y. (eds) Arbuscular Mycorrhizas: Physiology and Function. 5771. https://doi.org/ 10.1007/978-90-481-9489-6_3

Giovannetti M, Avio L, Sbrana C. 2010. Fungal spore germination and pre-symbiotic mycelial growth - physiological and genetic aspects. In: Koltai H., Kapulnik Y. (eds) Arbuscular Mycorrhizas: Physiology and Function. 332. https://doi.org/ 10.1007/978-90-481-9489-6_1

Giovannetti M, Mosse. 1980. An evaluation technique for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytologist. 84(3): 489500. https://doi.org/10.1111/j.1469-8137.1980.tb04556.x

Giovannini L, Palla M, Agnolucci M, Avio L, Sbrana C, Turrini A, Giovannetti M. 2020. Arbuscular Mycorrhizal Fungi and Associated Microbiota as Plant Biostimulants: Research Strategies for the Selection of the Best Performing Inocula. Agronomy. 10(106): 125. https://doi.org/10.3390/ agronomy10010106

Gobinath R, Ganapathy GP, Akinwumi II. 2015. Evaluating the use of lemon grass roots for the reinforcement of a landslide affected soil from Nilgris district, Tamil Nadu, India. Journal of Materials and Environmental Science. 6: 26812687.

Hajiboland R. 2013. Role of arbuscular mycorrhiza in amelioration of salinity. Salt Stress in Plants. 301354. https://doi.org/10.1007/978-1-4614-6108-1_13

Jefwa JM, Esther K, Turop L, Joseph M, Wilson N, Stephen MI, Nteranya S, Bernard V. 2012. Arbuscular mycorrhizal fungi in the rhizosphere of banana and plantain and the growth of tissue culture cultivars. Agriculture, Ecosystems and Environment. 157: 2431. https://doi.org/10.1016/ j.agee.2012.03.014

Karthikeyan A, Muthukumar T, Udaiyan K. 2005. Response of tea (Camellia sinensis (L). Kuntze) to arbuscular mycorrhizal fungi under plantation nursery conditions. Biological Agriculture & Horticulture. 22(4): 305319. https://doi.org/10. 1080/01448765.2005.9755294

Kumar A, Gupta A, Aggarwal A, Bhargav V. 2020. Efficacy of bioinoculants on biomass, nutritional status and yield of lemon grass, Cymbopogon citratus (DC.) Stapf. Journal of Spices and Aromatic Crops. 29(1): 5966. https://doi.org/10.25081/ josac.2020.v29.i1.6004

Lambraño RH, Nerlis PS, Karina CG, Stashenko E, Jesus OV. 2015. Essential oils from plants of the genus Cymbopogon as natural insecticides to control stored product pests. Journal of Stored Products Research. 63: 8283.

Li AM, Li SH, Wu XJ, Zhang J, He AN, Zhao G, Yang X. 2016. Effect of light intensity on leaf photosynthetic characteristics and accumulation of flavonoids in Lithocarpus litseifolius (Hance) Chun. (Fagaceae). Open Journal of Forestry. 6: 445459. https://doi.org/10.4236/ojf.2016.65034

McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA. 1990. A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol. 115(3): 495501. https://doi.org/10.1111/j.1469-8137.1990.tb00476.x

Muthukumar T, Sampath P. 2009. Arbuscular mycorrhizal morphology in crops and associated weeds in tropical agro-ecosystems. Mycoscience. 50(3): 233239. https://doi.org/10.1007/S10267-008-0475-8

Parapasan Y, Adryade RG. 2014. Waktu dan cara aplikasi cendawan mikoriza arbuskular (CMA) pada pertumbuhan bibit tanaman kopi. Jurnal Penelitian Pertanian Terapan. 13(3): 203208.

Paterson E, Allan S, Jane D, Timothy JD. 2016. Arbuscular mycorrhizal hyphae promote priming of native soil organic matter mineralization. Plant Soil. 408: 243. https://doi.org/10.1007/s11104-016-2928-8

Paz C, Öpik M, Bulascoschi L, Bueno CG, Galetti M. 2020. Dispersal of Arbuscular Mycorrhizal Fungi: Evidence and Insights for Ecological Studies. Microbial Ecology. 81(2): 110. https://doi.org/ 10.1007/s00248-020-01582-x

Perrin PM, Fraser JG, Mitchell. 2013. Effects of shade on growth, biomass allocation and leaf morphology in European yew (Taxus baccata L.). The European Journal of Forest Research. 132(2): 211218. https://doi.org/10.1007/s10342-012-0668-8

Pfeffer PE, David DD, Jr. Guillaume B, Yair Shachar-Hill. 1999. Carbon uptake and the metabolism and transport of lipids in an arbuscular mycorrhiza. Plants Physiol. 120(2): 587598. https://doi.org/ 10.1104/pp.120.2.587

Ragupathy S, Mahadevan A. 1991. VAM distribution influenced by salinity gradient in a coastal tropical forest. In Proceeding of second Asian Conference on Mycorrhiza. BIOTROP Special Publication. 42: 9197.

Setiawati W, Rini M, Ahsol H. 2011. Laboratory and field evaluation of essential oils from Cymbopogon nardus as oviposition deterrent and ovicidal activities against Helicoverpa armigera Hubner on chili pepper. Indonesian Journal of Agricultural Science. 12(1): 916. https://doi.org/10.21082/ ijas.v12n1.2011.p9-16

Sghir F, Jihane T, Mohamed C, Amina OT, Abdelkarim FM, Cherkaoui EM, Abdelmajid M, Ahmed O, Rachid B, Allal D. 2014. Diversity of arbuscular mycorrhizal fungi in the rhizosphere of date palm tree (Phoenix dactylifera) in Tafilalt and Zagora regions (Morocco). Indian Journal of Pure & Applied Biosciences. 2(6): 111.

Shi G, Yongjun L, Nancy CJ, Pål AO, Lin M, Gang C, Shengjing J, Lizhe A, Guozhen D, Huyuan F. 2014. Interactive influence of light intensity and soil fertility on root-associated arbuscular mycorrhizal fungi. Plant and Soil. 378(12): 173188. https://doi.org/ 10.1007/s11104-014-2022-z

Shukla A, Kumar A, Jha A, Chaturvedi OP, Prasad R, Ajit G. 2008. Effects of shade on arbuscular mycorrhizal colonization and growth of crops and tree seedlings in Central India. Agroforestry Systems. 76(1): 95109. https://doi.org/10.1007 /s10457-008-9182-x

Smith FA, Smith SE. 1997. Structural diversity in (vesicular)-arbuscular mycorrhizal symbioses. New Phytol. 137: 373388. https://doi.org/10.1046/ j.1469-8137.1997.00848.x

Smith SE, Read DJ. 2008. Mycorrhizal Symbiosis. San Diego (USA): Academic Press.

Suwitchayanon P, Osamu O, Kiyotake S, Hisashi KN. 2017. N-Octanoyl tyramine, a phytotoxic compound in the roots of Cymbopogon nardus. Acta Physiol Plant. 39(6): 123129. https://doi.org/10.1007/ s11738-017-2419-4

Voriskova A, Jansa J, Puschel D, Vosatka M, Smilauer P, Janouskova M. 2019. Abiotic contexts consistently influence mycorrhiza functioning independently of the composition of synthetic arbuscular mycorrhizal fungal communities. Mycorrhiza. 113. https://doi.org/10.1007/s00572-018-00878-8

Zangaro W, Leila VR, Priscila BdS, Ricardo dAA, Luiz EAML, Artur BLR, Marco AN, Rosilaine C. 2013. Root colonization and spore abundance of arbuscular mycorrhizal fungi in distinct successional stages from an Atlantic rainforest biome in southern Brazil. Mycorrhiza. 23: 221233. https://doi.org/ 10.1007/s00572-012-0464-9

Zheng C, Ji B, Zhang J, Zhang F, Bever JD. 2014. Shading decreases plant carbon preferential allocation towards the most beneficial mycorrhizal mutualist. New Phytologist. 205(1): 361368. https://doi.org/10.1111/nph.13025