Phytochemical Contents of Torbangun (Coleus amboinicus Lour) from Fractionation of Pressurized Liquid Extraction

Farida Laila; Dedi Fardiaz; Nancy Dewi Yuliana; Muhammad Rizal Martua  Damanik; Fitriya Nur Anissa Dewi

doi:10.18343/jipi.25.2.224

Farida Laila Department of Food Science and Technology, Faculty of Agricultural Technology, IPB University, IPB Darmaga Campus, Bogor 16680 and School of Vocational Studies, IPB University, Jl. Kumbang No 14, Bogor 16151 http://orcid.org/0000-0002-6690-8105
Dedi Fardiaz Department of Food Science and Technology, Faculty of Agricultural Technology, IPB University, IPB Darmaga Campus, Bogor 16680
Nancy Dewi Yuliana Department of Food Science and Technology, Faculty of Agricultural Technology, IPB University, IPB Darmaga Campus, Bogor 16680 http://orcid.org/0000-0003-0249-3753
Muhammad Rizal Martua Damanik Department of Community Nutrition, Faculty of Human Ecology, IPB University, IPB Darmaga Campus, Bogor 16680 http://orcid.org/0000-0002-8700-2185
Fitriya Nur Anissa Dewi Primate Research Center, Bogor Agricultural University, Jl. Lodaya II/5, Bogor 16151

DOI: https://doi.org/10.18343/jipi.25.2.224

Abstract

Coleus amboinicus (Lour) (torbangun) has been used traditionally as a breast milk stimulant, flavoring agent in many cuisines, and reported to possess many pharmacological values. The aim of this study was to explore the utility of the Pressurized Liquid Extraction (PLE) technique to identify the phytochemical contents of torbangun. In this study, total phenolic content and flavonoid in torbangun were determined using spectrophotometric method. The GC-MS analysis was used to identify the chemical constituents of torbangun, which was sequentially extracted with a wide range of solvent or solvent mixture from nonpolar-moderately polar and finally polar solvent. Total phenolic and flavonoid contents in the methanol extract of torbangun were 42.17±2.96 mg GAE/g and 11.20±0.58 mg QE/g, respectively. The identified chemical constituents in torbangun were sugar, hydrocarbon, ketone, terpenes, phenolics, fatty acid, fatty alcohol, steroids, alkaloids, and others, whereas the dominant constituents were phenolic compound, alkane, and sugar. In conclusion, this study demonstrated the effectiveness and rapid extraction of the PLE technique. Many valuable compounds in torbangun were extracted using gradual composition of solvent and were able to identify certain compounds in different polarities of solvents that are important in functional food preparation, pharmaceutical, and metabolomics research.

Keywords: bioactive, extract, nontoxic, plant, solvent

Downloads

Download data is not yet available.

References

Amalia NR, Damanik MRM. 2018. Torbangun Leaves (Coleus Amboinicus Lour) Powder Capsules Supplementation Improve Lipid Profile and Blood Pressure in Men with Hypercholesterol. Jurnal Gizi Dan Pangan. 13(2): 71–78. https://doi.org/10.25182/jgp.2018.13.2.71-78.

Amar Y, Meddah B, Bonacorsi I, Costa G, Pezzino G. 2017. Phytochemicals, Antioxidant and Antiproliferative Properties of Rosmarinus Officinalis L on U937 and CaCo-2 Cells. 16: 315–327.

Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, Jahurul MHA, Ghafoor K,Norulaini NAN, Omar AKM. 2013. Techniques for Extraction of Bioactive Compounds from Plant Materials: A Review. Journal of Food Engineering. 117(4): 426–436. https://doi.org/10.1016/j.jfoodeng.2013.01.014.

Balasundram N, Sundram K, Samman S. 2006. Phenolic Compounds in Plants and Agri-Industrial by-Products: Antioxidant Activity, Occurrence, and Potential Uses. Food Chemistry. 99(1): 191–203. https://doi.org/10.1016/j.foodchem.2005.07.042.

Bhatt P, Joseph GS, Negi PS, Varadaraj MC. 2013. Chemical Composition and Nutraceutical Potential of Indian Borage (Plectranthus Amboinicus) Stem Extract 2013.

Dai J, Mumper RJ. 2010. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules. 15(10): 7313–7352. https://doi.org/10.3390/molecules15107313.

Damanik R. 2009. Torbangun (Coleus Amboinicus Lour): A Bataknese Traditional Cuisine Perceived as Lactagogue by Bataknese Lactating Women in Simalungun, North Sumatera, Indonesia. Journal of Human Lactation. 25(1): 64–72. https://doi.org/10.1177/0890334408326086.

El-hawary SS, El-sofany RH, Abdel-Monem AR, Ashour RS. 2012. Phytochemical Screening, DNA Fingerprinting, and Nutritional Value of Plectranthus Amboinicus (Lour.) Spreng. Pharmacognosy Journal. 4(30): 10–13. https://doi.org/10.5530/pj.2012.30.2.

Hemalatha R, Nivetha P, Mohanapriya C, Sharmila G, Muthukumaran C. 2016. “Phytochemical Composition, GC-MS Analysis , in Vitro Antioxidant and Antibacterial Potential of Clove Flower Bud (Eugenia Caryophyllus) Methanolic Extract.” Journal of Food Science and Technology 53 (February): 1189–1198. https://doi.org/10.1007/s13197-015-2108-5.

Ivanov I, Dincheva I, Badjakov I, Petkova N, Denev P, Pavlov A. 2018. GC-MS Analysis of Unpolar Fraction from Ficus Carica L. (Fig) Leaves. International Food Research Journal. 25(1): 282–286.

Kellogg JJ, Wallace ED, Graf TN, Oberlies NH, Cech NB. 2017. Conventional and Accelerated-Solvent Extractions of Green Tea (Camellia Sinensis) for Metabolomics-Based Chemometrics. Journal of Pharmaceutical and Biomedical Analysis. 145: 604–610. https://doi.org/10.1016/j.jpba.2017.07.027.

Kim S, Thiessen PA, Bolton EE, Chen J, Fu G, Gindulyte A, Han L, et al. 2016. PubChem Substance and Compound Databases. Nucleic Acids Research. https://doi.org/10.1093/nar/gkv951.

De L.e. MS, David JP, Silva LCRC, Santos RAF, David JM, Lima LS, Reis PS, Fontana R. 2012. Bioactive Oleanane, Lupane and Ursane Triterpene Acid Derivatives. Molecules. 17(10): 12197–12205. https://doi.org/10.3390/molecules171012197.

Ls R, Nja S, Ncp S, Mc M, Aj T. 2016. Anticancer Properties of Phenolic Acids in Colon Cancer–A Review. 6(2). https://doi.org/10.4172/2155-9600.1000468.

Majumder P, Bhattacharjee P. 2013. Investigation of Phytochemicals and Anti-Convulsant Activity of the Plant Coleus Amboinicus (Lour.). International Journal of Green Pharmacy. 7(3): 211. https://doi.org/10.4103/0973-8258.120223.

Mustafa A, Turner C. 2011. Analytica Chimica Acta Pressurized Liquid Extraction as a Green Approach in Food and Herbal Plants Extraction : A Review. Analytica Chimica Acta. 703(1): 8–18. https://doi.org/10.1016/j.aca.2011.07.018.

NCATS 2018. National Center for Advancing Translational Sciences. US Department of Health and Human Services. National Institute of Health. Available on https://drugs.ncats.io

NParks Flora & Fauna Web. 2013. Plectranthus Amboinicus (Lour.) Spreng. Available on https://florafaunaweb.nparks.gov.sg/Special-Pages/plant-detail.aspx?id=3717.

Shubha JR, Bhatt P. 2015. Plectranthus Amboinicus Leaves Stimulate Growth of Probiotic L . Plantarum : Evidence for Ethnobotanical Use in Diarrhea. Journal of Ethnopharmacology. 166: 220–227. https://doi.org/10.1016/j.jep.2015.02.055.

Suryowati T, Damanik R, Bintang M. 2015. Antihyperlipidemic Activity of Torbangun Extract (Coleus Amboinicus Lour) on Diabetic Rats Induced by Streptozotocin. IOSR Journal Of Pharmacy. 5(5): 50–54.

Suryowati T, Rimbawan, Damanik R, Bintang M, Handharyani E. 2015. Identifikasi Komponen Kimia dan Aktivitas Antioksidan dalam Tanaman Torbangun (Coleus Amboinicus Lour). Jurnal Gizi Dan Pangan. 10(3): 217–224.

Yulianto W, Andarwulan N, Giriwono PE, Pamungkas J. 2016. HPLC-Based Metabolomics to Identify Cytotoxic Compounds from Plectranthus Amboinicus (Lour.) Spreng against Human Breast Cancer MCF-7Cells. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. https://doi.org/10.1016/j.jchromb.2016.10.024.