Karakteristik Isolat Bakteri Penghasil Dekstran dari Batang Tebu (Saccharum officinarum L.)

  • Sitti Rahbiah Akram Sekolah Pascasarjana, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
  • Titi Candra Sunarti Departemen Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
  • Anja Meryandini Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Keywords: dextran, dextransucrase, Paenibacillus polymyxa, sugar cane


Dextran is a long chain of carbohydrate polymers synthesized by dextransucrase enzyme with sucrose as a substrate. The physical, chemical conditions, temperature, and the concentration of sucrose in the isolate bacterial growth media play an important role in dextransucrase production. This study aims to isolate and characterize the dextransucrase producing bacteria. The bacteria were isolated from sugar cane. TBT 3.2 Isolate which had the highest viscosity (1.48 cP) and crude dextran (7.87 g/L) production was selected. TBT 3.2 Isolate was identified as Paenibacillus polymyxa with 99% similar identity. The Paenibacillus polymyxa TBT 3.2 was characterized based on their ability to produce dextran and cell number. The bacteria had the optimum growth conditions on the media for producing dextran at pH 7, temperature 30°C, and concentration of 20% sucrose. The highest dextransucrase activity and the highest specific activity were obtained after 25 minutes of incubation, with the activities of 29.41 mU/mL and 0.46 U/mg. Based on the results of this study, the Paenibacillus polymyxa TBT 3.2 can be used as a dextran producing bacteria.


Download data is not yet available.


Aman A, Siddiqui NN, Qader SA. 2012. Characterization and potential applications of high molecular weight dextran produced by Leuconostoc mesenteroides AA1. Carbohydrate Polymers. 87: 910-15. https://doi.org/10.1016/j.carbpol.2011.08. 094

Ash C, Priest FG, Collins MD. 1993. Molecular identifi cation of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie Van Leeuwenhoek. 64: 253-260. https:// doi.org/10.1007/BF00873085

Baktir A, Koiriyah Z, Rohman A. 2005. A thermophilic microbe producing dextranase from heated sugar cane. Indonesian Journal of Chemistry. 5(3): 224-227.

Belder AN. 2003. Dextran. Handbook from Amersham Biosciences. Sweden (SE): Uppsala University.

Dimic GR. 2006. Characteristics of the Leuconostoc mesenteroides subsp. mesenteroides strains from fresh vegetables. APTEFF. 37: 3-11. https:// doi.org/10.2298/APT0637003D

Falconer DJ, Mukerjea R, Robyt JF. 2011. Biosynthesis of dextrans with different molecular weights by selecting the concentration of Leuconostoc mesenteroides B-512FMC dextransucrase, the sucrose concentration, and the temperature. Carbohydrate Research. 346: 280-284. https://doi.org/ 10.1016/j.carres.2010.10.024

Gao S, Lewis GD, Ashokkumar M, Hemar Y. 2013. Inactivation of microorganisms by low-frequency high-power ultrasound: 1. Effect of growth phase and capsule properties of the bacteria. Ultrason Sonochem. 2013: 1-8.

Han J, Hang F, Guo B, Liu Z, You C, Wu Z. 2014. Dextran synthesized by Leuconostoc mesenteroides BD1710 in tomato juice supplemented with sucrose. Carbohydrate Polymers. 112: 556-562. https://doi.org/ 10.1016/j.carbpol.2014. 06.035

Hasan Z. 1999. Isolasi dan karekterisasi dekstransukrase dari isolat bakteri batang tebu. [Tesis]. Bogor (ID): Institut Pertanian Bogor.

Karthikeyan RS, Rakshit SK, Baradarajan A. 1996. Optimization of batch fermentation condition for dextran production. Bioprocess Engineering. 15: 247-251. https://doi.org/10.1007/BF02391585

Khalikova E, Susi P, Korpela T. 2005. Microbial dextran-dydrolyzing enzymes: fundamentals and applications. Microbiology and Molecular Biology Reviews. 69(2): 306-324. https://doi.org/ 10.1128/MMBR.69.2.306-325.2005

Kim D, Robyt JF, Lee SY, Lee JH, Kim YM. 2003. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of Leuconostoc mesenteroides B-512FMCM dextransucrase. Carbohydrate Research. 338: 1183-1189. https:// doi.org/10.1016/S0008-6215(03)00148-4

Lal S, Tabacchioni S. 2009. Ecological and Biotechnological Potencial of Paenibacillus polymyxa: a minireview. Indian of Journal Microbiology. 49: 2-10. https://doi.org/10.1007/ s12088-009-0008-y

Lawford GG, Kligerman A, Williams T. 1979. Dextranbiosynthesis of dextransucrase production by continous culture of Leuconostoc mesenteroides. Biotechnology and Bioengineering. 21: 1121-1131. https://doi.org/10.1002/bit.26021 0704

Lee JM, Fox PF. 1985. Purification and characterization of Paecilomyces lilacinus dextranase. Enzyme Microbiology Technology. 7: 573-577. https://doi.org/10.1016/01410229(85) 90103-6

Martinez-espindola JP, Iopez-munguia CA. 1985. On the kinetics of dextransucrase and dextran synthesis in batch reactors. Biotechnology Letters. 7(7): 483-486. https://doi.org/10.1007/BF01199863

Moulis C, Gilles J, David H, Emeline F, Gabrielle PV, Pierre M, Magali RS. 2006. Understanding the polymerization mechanism of glycoside-hydrolase family 70 glucansucrases. Journal of Biological Chemistry. 281(42): 31.254-31.267.

Miyagawa Y, Seto H, Ohto K, Kawakita H. 2012. Giant dextran partivles formed by dextransucrase immobilized on a tube surface in laminar flow. Biochemical Engineering Journal. 64: 17-21. https://doi.org/ 10.1016/j.bej.2012.02.008

Nuryadi MM, Istiqomah T, Faizah R, Uabidillah S, Mahmudi Z. 2013. Isolasi dan identifikasi bakteri asam laktat asal youghurt. Journal University of Jember. 1(5): 1-11.

Olcer z, Tanriseven A. 2010. Co-immobilization of dextransucrase and dextranase in alginate. Process Biochemistry. 45: 1.645-1.651.

Padmanabhan PA, Kim DS. 2002. Production of insoluble dextran using cell-bound dextransucrase of Leuconostoc mesenteroides NRRL B-523. Carbohydrate Research. 337: 1529-1533. https://doi.org/ 10.1016/S0008-6215(02)00214-8

Pennell RD, Barker PE. 1992. The production of the enzyme dextransucrase using unaerated continous fermentation. Journal of Chemical Technology and Biotechnology. 53: 21-27. https://doi.org/10.1002/ jctb.280530104

Pontoh J, Mirah G, Karundeng P, Kamuh V. 2012. Metode analisa dextran dalam nira dan gula aren. Bul Pal. 13(2): 109-114.

Purama RK, Goyal A. 2008. Screening and optimization of nutritional factors for higher dextransucrase production by Leuconostoc mesenteroides NRRL B-640 using statistical approach. Bioresource Technology. 99: 7.108-7.114. https://doi.org/10.1016/j.biortech. 2008.01.032

Roberts IS. 1996. The biochemistry and genetics of capsular polysaccharide production in bacteria. Annuak Review of Microbiology. 50: 285-315. https://doi.org/ 10.1146/annurev.micro.50.1.285

Robyt JF, Yoon SH, Mukerjea R. 2008. Dextransucrase and the mechanism for dextran biosynthesis. Carbohydrate Research. 343: 3.039-3.048. https:// doi.org/ 10.1016/j.carres.2008.09.012

Sarwat F, Qader SA, Aman A, Ahmed N. 2008. Production & characterization of a unique destran from an indigenous Leuconostoc mesenteroides CMG713. International Journal of Biological Sciences. 4: 379-386. https://doi.org/10.7150/ ijbs.4.379

Son MJ, Jang EK, Kwon OS, Seo JH, Kim IJ, Lee IS. 2008. Characterization of dextran produced from Leuconostoc citreum S5 strain isolated from Korean fermented vegetable. European Food Research and Technology. 226(4): 697-706. https:// doi.org/10.1007/s00217-007-0579-y

Tahir MN. 2012. Alkynyl ethers of dextrans as intermediates for new functional biopolymers. [Disertasi]. Braunschweig (DE): Technische Universität Braunschweig.

Yang Y, Peng Q, Guo Y, Han Y, Xiao H, Zhou Z. 2015. Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut. Carbohydrate Polymers. 133: 365-372. https://doi.org/10.1016/j.carbpol.2015.07.061

Zahnley JC, Smith MR. 1995. Insoluble glucan formation by Leuconostoc mesenteroides NRRL B-1355. Applied and Environmental Microbiology. 61: 1.120-1.123.

Zohra RR, Aman A, Zohra RR, Ansari A, Ghani M, Qader SAU. 2013. Dextranase: hyper production of dextran degrading enzyme from newly isolated strain of Bacillus licheniformis. Carbohydrate Polymers. 92: 2.149-2.153. https://doi.org/10.1016/ j.carbpol.2012.11.044

How to Cite
AkramS. R., SunartiT. C., & MeryandiniA. (2019). Karakteristik Isolat Bakteri Penghasil Dekstran dari Batang Tebu (Saccharum officinarum L.). Jurnal Ilmu Pertanian Indonesia, 24(2), 160-167. https://doi.org/10.18343/jipi.24.2.160