Evaluasi Ketahanan Galur International Rice Bacterial Blight terhadap Hawar Daun Bakteri dan Identifikasi Patotipe Xanthomonas oryzae pv. oryzae Provinsi Jawa Tengah Berdasarkan Galur IRBB
Abstract
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is the major disease in rice plants causing considerable economic losses. The objective of the study was to evaluate the resistant International Rice Bacterial Blight (IRBB) lines against bacterial leaf blight and the identification of Xoo pathotype from Central Java Province as the basis recommendation of the resistant varieties assembly with the background of resistant genes to overcome BLB disease. The evaluation of Xoo isolates from 22 districts in Central Java Province on IRBB lines was conducted in the rainy season 2016-2017 using split plot with three replications. The main plot was 15 IRBB lines, while the subplot was 110 Xoo isolates. Among 15 lines of IRBB, there were 10 lines have functioned as pathotype examiner of Xoo. The results obtained 6 lines were reacted resistant, namely IRBB5, IRBB7, IRBB8, IRBB57, IRBB64, and IRBB66. The IRBB66 line can be used as a resistant elder for the assembly of BLB resistant varieties because it has a combination of pyramiding resistant genes that were effective against BLB in some areas of Central Java Province. Pathotype IX was only found and dominant in Pemalang. Pathotype X was obtained in six districts of Tegal, Brebes, Banjarnegara, Purbalingga, Purworejo, and Grobogan. Pathotype XII was the most dominant prototype in 15 districts. Assembling of resistant varieties with resistant gene backgrounds based on the existence of a specific Xoo pathotype in the field can reduce the severity of BLB disease.
Downloads
References
Adhikari TB, Vera Cruz CM, Zhang Q, Nelson RJ, Skinner DZ, Mew TW, Leach JE. 1995. Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia. Applied and Environmental Microbiology. 61(3): 966-971.
Asysyuura, Nawangsih AA, Mutaqin KH, Sudir. 2017. Identifikasi patotipe Xanthomonas oryzae pv. oryzae dari tanaman padi di Sulawesi Selatan. Jurnal Fitopatologi Indonesia. 13(3): 73-80. http://doi.org/cmk7
Deng WL, Lin HA, Shih YC, Kuo CC, Tzeng JY, Liu LYD, Huang ST, Huang CM, Chung CL. 2016. Genotypic and pathotypic diversity of Xanthomonas oryzae pv. oryzae strains in Taiwan. Journal of Phytopathology. 164(10): 745-759. http://doi.org/cmk8
Djatmiko HA, Prakoso B, Prihartiningsih N. 2011. Penentuan patotipe dan keragaman genetik Xanthomonas oryzae pv. oryzae pada tanaman padi di wilayah Karesidenan Banyumas. Jurnal Hama dan Penyakit Tumbuhan Tropika Tropika. 11(1): 35-46.
George MLC, Bustamam M, Cruz WT, Leach JE, Nelson RJ. 1997. Movement of Xanthomonas oryzae pv. oryzae in Southeast Asia detected using PCR-based DNA fingerprinting. Phytopathology 87(3): 302-309. http://doi.org/cgjx7r
Hadianto W, Hakim L, Bakhtiar. 2015. Ketahanan beberapa genotipe padi terhadap penyakit hawar daun bakteri (Xanthomonas oryzae pv. oryzae). Jurnal Hama dan Penyakit Tumbuhan Tropika. 15(2): 152-163. http://doi.org/cmk9
Hifni HR, Kardin MK. 1998. Pengelompokkan isolat Xanthomonas oryzae pv. oryzae dengan menggunakan galur isogenik padi IRRI. Hayati. 5(3): 66-72.
Jamil A, Satoto, Sasmita P, Baliadi Y, Guswara A, Suharna. 2015. Deskripsi Varietas Unggul Baru padi. Jakarta (ID): Badan Penelitian dan Pengembangan Pertanian. Kementerian Pertanian.
Khoshkdaman M, Ebadi AA, Shilsar FM, Dariush S. 2014. Preliminary evaluation of resistance genes in rice against bacterial leaf blight in Gullan Province-Iran. Agriculture Sciences. 5(2): 94-98. http://doi.org/cmmb
Lai VE, Noda T, Pham VD. 1999. Resistance assessment of rice cultivars to Xanthomonas oryzae pv. oryzae and pathogenicity testing of bacterial leaf blight isolates in Vietnam. Omonrice. 7: 120-131.
Lee KS, Rasabandith S, Angeles ER, and Kush GS. 2003. Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology. 93(2): 147-152. http://doi.org/fw3p9j
Lore JS, Vikal Y, Hunjan MS, Goel RK, Bharaj TS, Raina GL. 2012. Genotypic and pathotypic diversity of Xanthomonas oryzae pv. oryzae, the cause of bacterial blight of rice in Punjab State of India. Journal of Phytopathology. 159: 479-487. http://doi.org/b8gbcx
Nayak B, Reddy PR, Nayak P. 2009. Variability in Xanthomonas oryzae pv. oryzae, the incitant of bacterial leaf blight of rice. Journal of Plant Protection Research. 49(1): 15-26. http://doi.org/d53jc2
Nino-Liu D, Ronald PC, Bogdanove AJ. 2006. Xanthomonas oryzae pathovars: model pathogens of a model crop. Molecular Plant Pathology. 7(5): 303-324. http://doi.org/cvp2f2
Noda T, Horino O, Ohuchi A. 1990. Variability of pathogenicity in races of Xanthomonas campestris pv. oryzae in Japan. Japan Agricultural Research Quarterly. 23(3): 182-189.
Sattari A, Fakheri B, Noroozi M, Gudarzi M. 2014. Leaf blight resistance in rice: a review of breeding and biotechnology. International Journal of Farming and Allied Sciences. 3(8): 895-902.
Sudir, Sutaryo B. 2011. Reaksi padi hibrida introduksi terhadap penyakit hawar daun bakteri dan hubungannya dengan hasil gabah. Jurnal Penelitian Pertanian Tanaman Pangan. 30(2): 88-94.
Sudir, Nuryanto B, Kadir TS. 2012. Epidemiologi, patotipe, dan startegi pengendalian penyakit hawar daun bakteri pada tanaman padi. Buletin IPTEK Tanaman Pangan. 7(2): 79-87.
Suparyono, Sudir, Suprihanto. 2003. Komposisi patotipe patogen hawar daun bakteri pada tanaman padi stadium tumbuh berbeda. Jurnal Penelitian Pertanian Tanaman Pangan. 22(1): 45-50. http://doi.org/cmmc
Suparyono, Sudir, Suprihanto. 2004. Pathotype profile of Xanthomonas oryzae pv. oryzae isolates from the rice ecosystem in Java. Indonesian Journal of Agricultural Science. 5(2): 63-69.
Tasliah. 2012. Gen ketahanan tanaman padi terhadap bakteri hawar daun (Xanthomonas oryzae pv. oryzae). Jurnal Litbang Pertanian 31(3): 103-112. http://doi.org/cmmd
Tasliah, Mahrup, Prasetiyono J. 2013. Identifikasi molekuler hawar daun bakteri (Xanthomonas oryzae pv. oryzae) dan uji patogenisitasnya pada galur-galur padi isogenik. Jurnal Agro Biogen. 9(2): 49-57.
Vera CCM, Bai J, Ona I, Leung H, Nelson RJ, Mew TW, Leach JE. 2000. Predicting durability of a disease resistance gene based on an assessment of the fitness loss and epidemiological consequences of avirulence gene mutation. Proceedings of the National Academy of Sciences. 97(25): 13500-13505. http://doi.org/dzhhpv
This journal is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License. Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. NonCommercial — You may not use the material for commercial purposes.
View JIPI StatsCounter
