Enhanced Biodegradation of DDT by White-Rot Fungus Phlebia brevispora TMIC34596 in a Nutrient-Rich Medium at Short Incubation Time

Erly Grizca Boelan; Adi Setyo  Purnomo; Fajriah; Ichiro  Kamei; Maria Aloisia Uron  Leba; Hildegardis  Missa; Anselmus Boy  Baunsele; Aloysius Masan  Kopon

doi:10.4308/hjb.33.1.250-255

Authors

Erly Grizca Boelan Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia
Adi Setyo Purnomo Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS Sukolilo, Surabaya 60111, Indonesia
Fajriah Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS Sukolilo, Surabaya 60111, Indonesia
Ichiro Kamei Department of Forest and Enviromental Sciences, Faculty of Agriculture, University of Miyazaki 1-1 Gakuen-kibanadai-nishi, Miyazaki, Japan
Maria Aloisia Uron Leba Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia
Hildegardis Missa Department of Biology Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia
Anselmus Boy Baunsele Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia
Aloysius Masan Kopon Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia

DOI:

https://doi.org/10.4308/hjb.33.1.250-255

Abstract

1,1,1-Trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) is a persistent organic pollutant that remains a global environmental concern due to its high toxicity and recalcitrance. This study investigated the ability of the white-rot fungus Phlebia brevispora to degrade DDT in a nutrient-rich Potato Dextrose Broth (PDB) medium under a short incubation time. The fungus degraded 64.25% of DDT within 7 days. Metabolites identified by GC/MS were DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene), indicating both reductive dechlorination and dehydrogenation pathways. The results demonstrate that P. brevispora has strong potential for rapid biotransformation of DDT in nutrient-rich environments.

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Author Biographies

Erly Grizca Boelan, Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia

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Adi Setyo Purnomo, Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS Sukolilo, Surabaya 60111, Indonesia

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Fajriah, Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS Sukolilo, Surabaya 60111, Indonesia

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Ichiro Kamei, Department of Forest and Enviromental Sciences, Faculty of Agriculture, University of Miyazaki 1-1 Gakuen-kibanadai-nishi, Miyazaki, Japan

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Maria Aloisia Uron Leba, Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia

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Hildegardis Missa, Department of Biology Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia

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Anselmus Boy Baunsele, Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia

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Aloysius Masan Kopon, Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Kupang 85211, Indonesia

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