The Use of HIS6 Gene as a Selectable Marker for Yeast Vector

Authors

  • I MADE ARTIKA

DOI:

https://doi.org/10.4308/hjb.16.1.40

Abstract

The yeast Saccharomyces cerevisiae HIS6 gene has been shown to be functional as a selectable marker for selecting and maintaining a yeast vector in yeast S. cerevisiae host cells. The yeast HIS6 gene encodes an enzyme involved in the yeast histidine biosynthesis. The yeast HIS6 gene was cloned into a yeast expression vector. The resultant recombinant plasmid was introduced into yeast host cells defective in endogenous HIS6 gene. The functionality of the HIS6 gene as a selectable marker was tested by growing transformed cells on selective minimum medium lacking histidine supplementation.

Key words: HIS6 gene, selectable marker, vector, transformation, yeast

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Issue

Vol. 16 No. 1 (2009): March 2009

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Articles

License

HAYATI J Biosci  is an open access journal and the article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation. Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/licenses/by-nc/4.0/ .  And Authors can still use their work commercially.

How to Cite

ARTIKA, I. M. (2009). The Use of HIS6 Gene as a Selectable Marker for Yeast Vector. HAYATI Journal of Biosciences, 16(1), 40. https://doi.org/10.4308/hjb.16.1.40
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