Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia

DEWI FITRIANI; BUDI SAKSONO

doi:10.4308/hjb.17.2.58

Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia

Authors

DEWI FITRIANI Research Center for Biotechnology, Indonesia Institute of Science
BUDI SAKSONO Research Center for Biotechnology, Indonesia Institute of Science

DOI:

https://doi.org/10.4308/hjb.17.2.58

Abstract

L-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesia. The amplified araA gene consisted of 1494 bp nucleotides encoding 497 amino acids. DNA alignment analysis showed that the gene had high homology with that of G. stearothermophilus T6. The enzyme had optimum activity at high temperature and alkalin condition.

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Issue

Vol. 17 No. 2 (2010): June 2010

Section

Articles

License

HAYATI J Biosci is an open access journal and the article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation. Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/licenses/by-nc/4.0/ . And Authors can still use their work commercially.

How to Cite

FITRIANI, D., & SAKSONO, B. (2010). Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia. HAYATI Journal of Biosciences, 17(2), 58. https://doi.org/10.4308/hjb.17.2.58