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Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, comptational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms. Manuscripts should be written and published in Bahasa or English and should be submitted to CB. Authors should refer to the guidline below when preparing their manuscripts. 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Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms. Manuscripts should be written and published in Bahasa or English and should be submitted to CB. Authors should refer to the guidline below when preparing their manuscripts. Previously published manuscripts (either in whole or in part) will not be considered.
Pengembangan Beras Putih Tinggi Protein Rendah Glikemik (RASPUTIN) untuk Penderita Diabetes Berbasis CRISPR/Cas9
Diabetes merupakan salah satu penyakit mematikan yang banyak menjangkit masyarakat Indonesia dengan jumlah penderita yang terus meningkat setiap tahunnya. Salah satu faktor yang menjadi pemicunya adalah konsumsi beras putih yang bernilai indeks glikemik tinggi sebagai makanan pokok sehari-hari. Salah satu alternatif untuk mengatasi masalah tersebut adalah dengan memproduksi beras RASPUTIN, yakni beras terekayasa genetik dengan indeks glikemik rendah. Penelitian ini bertujuan untuk mengetahui potensi beras RASPUTIN dalam menurunkan risiko diabetes serta cara memproduksi beras RASPUTIN secara efektif menggunakan CRISPR/Cas9. Penelitian ini menggunakan metode studi literatur yang bersumber dari artikel ilmiah dan jurnal penelitian. Gen OsSPL16 diisolasi dengan menggunakan bulir padi sebagai sampel. Sel dilisiskan dengan manual grinding dan diekstraksi menggunakan metode CTAB. Plasmid direkonstruksi dengan melibatkan promoter U6 dan CAG yang secara berturut-turut mampu mengekspresikan sgRNA dan SpCas9. Agrobacterium-delivered CRISPR/Cas9 digunakan sebagai model sel kompeten yang akan ditransfer ke dalam kecambah padi setelah dikloning pada media lysogeny broth (LB). Kecambah RASPUTIN dikultivasi dan tanaman dewasa diaklimatisasi. Analisis dilakukan secara kualitatif dan kuantitatif. Hasil yang diperoleh, yaitu diperoleh rancangan rekonstruksi plasmid serta desain sgRNA yang kompatibel untuk memproduksi beras putih termodifikasi secara genetik. Beras RASPUTIN diproduksi melalui teknologi rekayasa genetika CRISPR/Cas9 dapat menjadi solusi alternatif untuk menurunkan risiko terjadinya diabetes melitus.
Copyright (c) 2025 Danendra Daryl Abhinaya, Lutfiah Rahmadini Fitria, Syahid Fattah Asgasatya, Anto Budiharjo
INOVASI PRODUKSI BIOETANOL BERBASIS LIGNOSELULOSA LIMBAH KULIT KOPI (COFFEA SP.) DENGAN KONSEP BIOREFINERY MENGGUNAKAN SACCHAROMYCES CEREVISIAE
Indonesia is the fourth largest coffee exporter in the world, with a market share of around 11%. In 2021, Indonesia's coffee production reached 765,415 tons, generating 382,000 tons of coffee husk waste. However, only about 2% of this amount is utilized, coffee skins contain 20-50% cellulose which hasthepotential to used as a substrate for bioethanol production, a renewable energy source. This study aims to explore innovations in bioethanol production from lignocellulosic coffee pulp waste, particularly through a biorefinery method using Saccharomyces cerevisiae as fermentation agent. The research employs an exploratory descriptive approach, by reviewing relevant scientific articles. The process of converting lignocellulosic substrates into ethanol consists of four main stages in bioethanol production, namely: (1) pretreatment, which can be done physically, chemically, or biologically; (2) hydrolysis of polymers such as cellulose and hemicellulose into simple sugars such as hexose and silose; (3) fermentation of sugars by microorganisms to produce ethanol; and (4) separation and purification of the produced ethanol. The utilization of the biorefinery concept in bioethanol production not only facilitates the conversion of biomass into bioethanol, but also enables the production of other high-value products, such as biohydrogen (environmentally friendly fuel), furfural (resin and composite industry), HMF (bioplastic precursor and furan fuel), xylitol (sweetener in food and pharmaceutical industries), vinasse (organic fertilizer and biomass fuel), organic acids (chemical and pharmaceutical industries), and microbial biomass (high-protein animal feed). Keywords: bioethanol, biorefinery, coffee skin, lignocellulosic waste, saccharomyces cerevisiae
Copyright (c) 2025 Haikal Azmi, Nazla Fauziyah Octaviani, Musa'adah Musa'adah https://creativecommons.org/licenses/by-sa/4.0
IN SILICO STUDY OF ACTIVE COMPOUNDS IN Halosi (Bidens pilosa) AS ANDROGEN INHIBITORS IN PROSTATE CANCER
Prostate cancer is a disease that affects men's prostate gland cells and can spread to other parts of the body, mostly bones and lymph nodes. It is the third most common type of cancer in men in Indonesia and the second most common type of cancer in men worldwide. Ini adalah studi in silico tentang aktivitas chemical compounds in the leaves of the halosi plant (Bidens pilosa) to inhibit the Androgen receptor (1E3G) in the treatment of prostate cancer. Androgen receptor (AR) is a receptor protein that plays an important role in the development of prostate cancer cells. Molecular docking method using Chemdraw 3D, AutoDockTools 1.5.6., BIOVIA Discovery Studio 2021, and Ligandscout were used to conduct tests. Hasil menunjukkan bahwa bahan Bidens pilosa memiliki rendahnya ∆G free bond energy sebesar -10.26 KKal/mol and interaction with with several amino acid residues similar to Oxymetholone, a natural ligand. Therefore, it can be concluded that andrographolid compounds from halosi plant are the most able to inhibit Andorgen receptors
Copyright (c) 2025 Armansyah Maulana Harahap, Indra Priawan, Sarah Rahmayani Siregar
In Silico Analysis of Red Betel (Piper crocatum) Active Compounds as Xanthine Oxidase Inhibitors
Asam urat jika diproduksi secara berlebih oleh tubuh dapat mengakibatkan pembengkakan, rasa nyeri dan kaku dipersendian. Pembentukkan asam urat di tubuh dapat dihambat dengan cara menginhibisi kerja enzim xantin oksidase. Penelitian ini bertujuan mengidentifikasi senyawa aktif sirih merah yang dapat menginhibisi aktivitas enzim xantin oksidase melalui penambatan molekuler (in silico). Metode in silico yang dilakukan yaitu penambatan terarah (site directed docking) dengan menggunakan AutoDock Tools 1.5.6. Ukuran pusat penambatan molekuler sebesar x=26,7853; y= 9,9800; z= 113,3917 dengan nilai dimensi x=14 ; y=14 ; z=18. Senyawa aktif sirih merah terpilih adalah asam protokatekuat, sofalkon, dan 2-(3,4-dimetoksi fenil)-6-etoksi-7- metoksi-1-naftol karena mempunyai nilai ∆G dan Ki yang lebih kecil dibanding allopurinol. Selain itu, senyawa-senyawa aktif tersebut memiliki interaksi residu asam amino yang kuat terhadap sisi aktif xantin oksidase.
Copyright (c) 2022 Mega Safithri, Shafillah Miantika, Laksmi Ambarsari
Acetylcholinesterase Enzyme Inhibitor and Antioxidant Activities from A Mixture Extracts of Black Tea, Red Betel, Cinnamon and Curcuma
Alzheimer's Disease (AD) is one of the consequences of impaired functioning of acetylcholine which can be hydrolyzed by the enzyme acetylcholinesterase. Alzheimer's treatment is carried out using two approaches, namely compounds (acetylcholinesterase inhibitors) and noncholinergics (antioxidants). There are commercial drugs that can slow the progression of Alzheimer's, but their use can cause excessive side effects. The use of herbal plants as a cure for Alzheimer's disease has been proven to be safer and does not cause excessive side effects. Herbal plants that can be used and developed are black tea, red betel, curcuma, and cinnamon. This study determined that extracts of black tea, red betel leaf, curcuma, and cinnamon's formula have the best antioxidant activity and level of inhibition of the acetylcholinesterase enzyme. F1 (cinnamon), F5 (cinnamon, black tea, red betel, and curcuma), F6 (cinnamon and black tea), and F11 (cinnamon, black tea, and curcuma) were the best formulations in each type of sample based on inhibitory AChE enzymes and antioxidants activity. F1 (cinnamon) is the most effective extract out of all formulations analyzed in this study. Keywords: Acetylcholinesterase; Alzheimer; Black Tea; Cinnamon; Red Betel; Curcuma
Copyright (c) 2022 Aziz Syamsul Huda, Akhmad Endang Zainal Hasan, Mega Safithri
In Silico Analysis of Active Compounds of Avocado Fruit (Persea americana Mill.) as Tyrosinase Enzyme Inhibitors
Melanocytes are an important component of skin pigmentation through their ability to produce melanin assisted by the enzyme tyrosinase which is a core enzyme in melanogenesis. The high phenolic and flavonoid compounds in the leaves, seeds and skin of avocados have potential as ligands (inhibitors) of tyrosinase related protein-1. This study aims to determine the active compounds from the leaves, seeds, and skin of avocados that play a role in inhibiting the activity of the tyrosinase enzyme in humans so that it can inhibit the formation of melanin. The research was conducted by molecular anchoring method using YASARA Structure software. The results showed that of the 68 active compounds of avocado leaves, seeds, and skins, there were 11 active compounds that had binding affinity for tyrosinase related protein-1, namely quarcetin, epicatechin, apigenin, 3-Hydroxytyrosol, scopoletin, abscisic acid, citric acid, coumaric acid. , quinic acid, vanillin, and caffeic acid. The active compound abscisic acid is the ligand with the best inhibitory potential with a bond free energy (∆G) of -2,645 kcal/mol which interacts with 17 amino acid residues. The research results can be used as a reference in in vitro and in vivo studies of the human tyrosinase enzyme.
Copyright (c) 2022 Puspa Julistia Puspita, Laksmi Ambarsari, Ni Putu Peggy Liliyani
Sprague Dawley Rat Spermatozoa Quality Due to Bee Pollen Consumption
Bee Pollen is a food that has a complete nutritional content including as a source of steroid hormones, helping to improve concentration and brain performance. This study aims to determine the effect of bee pollen on the sperm quality of male rats. Administering 1 ml of water as control (-), 1 ml/kg of Eurycoma longifolia capsule as control (+), 1 ml/kg of nano bee pollen, and bee pollen with concentrations of 0.33 ml/kg, 0.66 ml/kg, 1 ml/kg, and 1.33 ml/kg 34 times in Sprague Dawley male white rats. The mice were then operated on and examined for testicular weight, epididymal weight, sperm pH, sperm motility, sperm concentration, and sperm abnormalities. The results showed that bee pollen and nano bee pollen did not significantly affect the quality of rat spermatozoa. In examining the testicular weight of rats, 0.33 ml/kg of bee pollen had an average testicular weight greater than the control group. For sperm weight and pH of the rat epididymis, the administration of bee pollen showed the average weight and pH were lower than the control. In the examination of sperm motility, 0.33 ml of bee pollen had a higher percentage than the control, while 1 ml of nano bee pollen had the lowest percentage of motility. The results of the concentration and abnormalities of rat spermatozoa from a dose of 1 ml/kg of bee pollen had an average sperm count higher than the control which was 2,129.7 million/ml and the lowest percentage of sperm abnormalities was 8.73%.
Copyright (c) 2022 Akhmad Endang Zainal Hasan, Moerfiah Moerfiah, Septa Fitri Handayani, Septa Fitri Handayani
Stress Simulation Analysis on 316L Stainless Steel Bone Plate for Biomaterial Applications
Biomaterial adalah material yang digunakan untuk mengganti struktur biologis yang hilang atau sakit. Material ini harus dapat diterima oleh tubuh. Salah satu biomaterial yang paling banyak digunakan untuk aplikasi bone plate adalah stainless steel 316L. Saat diaplikasikan pada tubuh pasien, material ini akan mendapatkan beban sejajar permukaan. Beban ini merupakan beban yang cukup dominan diterima oleh bone plate stainless steel 316L. Beban yang diterima bone plate stainless steel 316L akan berubah dan bervariasi. Sehingga perlu dilakukan simulasi tegangan degan memberikan gaya tekan pada bone plate stainless steel 316L. Simulasi tegangan dilakukan dengan menggambar dan menganalisis tegangan dengan menggunakan Autodesk Fusion 360. Selanjutnya material tersebut diberikan beban berupa static load mulai dari 150 N sampai 2300 N. Hasil analisis tegangan menunjukkan distribusi tegangan terdapat ditengah dan ujung bone plate. Regangan dan perubahan bentuk menunjukkan peningkatan seiring dengan kenaikan nilai tegangan. Gaya tekan 150 N sampai 600 N merupakan beban yang aman karena masih dibawah titik luluh 170 MPa. Gaya tekan 700 N sampai 2300 N merupakan beban yang tidak aman karena sudah melebihi titik luluhnya. Hal ini akan menyebabkan material mengalami deformasi plastis dan patah.
Copyright (c) 2023 Ahmad Fikri, Agam Muarif, Rizka Mulyawan, Nursakinah
Effectiveness of Bay Leaf Decoction (Syzygium polyanthum) on Reducing Blood Glucose Levels in Paok Motong, Masbagik, East Lombok
One disease that correlates with blood glucose levels is diabetes mellitus (DM). According to Basic Health Research (RISKESDAS), in Indonesia by 2030 there will be an increase of up to 21.3 million people suffering from diabetes mellitus. However, nowadays most people are afraid of the side effects of using the drugs they consume and are turning to herbal therapy to lower blood glucose levels. Bay leaves have the benefit of treating diabetes mellitus, hypercholesterolemia, hypertension, diarrhea and gastritis. Phytochemical analysis shows that bay leaves contain essential oils, tannins, flavonoids and terpenoids. The aim of the research was to determine the effect of bay leaf boiled water on reducing blood glucose levels. The research was an experimental One Group Pretest Post-test Design, namely the research subjects had their blood glucose levels measured before (pre-test) and after being given bay leaf boiled water (post-test). 41 respondents in Paok Motong Barat village, Masbagik District, East Lombok Regency had their Blood Glucose Levels (GDS) measured before (pre-test) and after (post-test) giving 300 mL of bay leaf boiled water. Paired Test Statistical Analysis was used to determine the difference in pretest and posttest blood glucose levels given bay leaf boiled water. The results of the study showed that there was a significant decrease in blood glucose levels (p= 0.001) by 15.22 mg/dL to 179.27 mg/dL before administering bay leaf decoction amounting to 194.49 mg/dL. The conclusion is that there is a significant effect (p=0.001) of giving boiled bay leaves on reducing blood glucose levels. The suggestion for this research is to increase the number of respondents in the research as well as examination variables such as fasting blood glucose
Copyright (c) 2024 Aji Kresnapati, Rozikin, I Putu Bayu Agus Saputra, Sabariah, I Nyoman Bagus Aji Kresnapati
Kandungan Nutrisi dan Daya Inhibisi α-glukosidase Ekstrak Daging Buah Salak Sidempuan (Salacca sumatrana)
Penelitian terhadap pemanfaatan salak khususnya salak Sidempuan sebagai inhibitor α-glukosidase belum dilakukan. Penelitian ini bertujuan mengukur kadar proksimat (air, abu, protein kasar, lemak kasar, serat kasar, dan karbohidrat), menganalisis komponen fitokimia, kandungan total fenolik dan aktivitas penghambatan α-glukosidase pada ekstrak air dan etanol daging buah salak Sidempuan. Kadar total fenol diukur menggunakan metode Folin Ciocalteu. Aktivitas penghambatan α-glukosidase diukur menggunakan substrat pNPG. Kadar karbohidrat, air, abu, lemak kasar, protein kasar, dan serat kasar berturut-turut sebesar 86.35%, 6.57%, 3.60%, 1.44%, 1.24%, dan 0.82%. Kedua ekstrak mengandung senyawa flavonoid dan saponin. Ekstrak etanol 70% daging buah salak Sidempuan memiliki kandungan total fenol sebesar 10.6 mg GAE/g ekstrak, lebih tinggi dibandingkan ekstrak air sebesar 5.55 mg GAE/g esktrak. Ekstrak etanol daging buah salak Sidempuan memiliki konsentrasi inhibisi (IC50) sebesar 13.69 mg/L. Konsentrasi ini lebih kecil dibandingkan ekstrak air yaitu 4160.56 mg/L, namun masih lebih besar dibandingkan akarbosa yaitu 1.15x10-3 mg/L sebagai kontrol positif.
Copyright (c) 2024 Mega Safithri, Aprilita Putri Defan Ritonga, Susi Indariani, Maheswari Alfira Dwicesaria, Sulistiyani, Hasim
Optimization of PCR Conditions for Adding XhoI Restriction Sites to the Glucose Oxidase Gene of Aspergillus niger IPBCC 08.610
Glucose oxidase (GOX) is naturally produced by fungi Aspergillus niger. The GOX enzyme catalyzes the oxidation reaction of β-D-glucose to produce δ-gluconolactone and hydrogen peroxide a (H2O2). Hydrolysis of δ-gluconolactone will produce gluconic acid. Gluconic acid and its derivatives have benefits in the pharmaceutical field as a drug for mineral deficiencies. A. niger IPBCC 08.610 is a local isolate that produce intracellular GOX with higher yield than extracellularly. GOX can be expressed extracellularly by cloning into the expression vector pPICZαB which has the signal peptide α-mating factor (α-MF). GOX gene construction needs to be done by adding some features such as XhoI restriction sites at the 5' and 3' ends, XbaI restriction site at 3’ side, and spacer peptide glu-ala-glu-ala at 5’ side. This research aims to optimize Polymerase Chain Reaction (PCR) conditions in two stages of amplification, stage I to copy the GOX gene and stage II to add those features so it is hoped that recombinant GOX can increase gluconic acid production. The results of primer concentration optimization showed that primers with a concentration of 10 µM produced clearer PCR amplicons than those with a concentration of 20 µM. The optimal temperature for amplification stage I is 58°C. The amplification stage II annealing temperature was modified with the first ten cycles based on the lowest Tm of primer value, 52°C, and the subsequent 25 cycles based on the highest Tm of primer value, 61°C.
Copyright (c) 2024 Hanifah Aryani, Nadhira Fathiaz Akbar, Popi Asri Kurniatin, Asrul Muhamad Fuad, Laksmi Ambarsari
Phytochemical and Organoleptic Tests of Combined Extracts of Turmeric, Black Tea, and Ginger
Phytochemical screening to conduct a preliminary evaluation of the chemical content of black tea (Camellia sinensis), Turmeric (Curcuma longa), and Ginger (Zingiber officinale). In addition, the content of secondary metabolites in black tea, turmeric and ginger can be used as an indication of the quality of these plants. the need for a combination of black tea extract act, turmeric, and ginger from various people so that it can be liked by the public. This study aims to test the phytochemical extracts of black tea, turmeric, and ginger as well as organoleptic tests on the most optimum combination of -glucosidase enzyme inhibitory activity. Phytochemical screening showed that black tea and ginger extract contains alkaloids, flavonoids, tannins, saponins, terpenoids, quinones, glycosides, and phenolics, while turmeric extract contains alkaloids, flavonoids, tannins, terpenoids, quinones, glycosides, and phenolics. The organoleptic test of the F7 combination consisting of a combination of black tea extract, turmeric, and ginger showed the highest level of preference by P3 and P2 on color, aroma, consistency, and aftertaste, while taste was at P3.
Copyright (c) 2024 Rara Annisaur Rosyidah, Dimas Andrianto
Combination of Pulai Leaf (Alstonia scholaris (L.) R. Br.) and Papaya Leaf (Carica papaya L.) Water Extract as HMG-CoA Reductase Inhibitor
Heart disease has become the leading cause of death in the world in the last 15 years. This disease has several primary risk factors, one of which is hypercholesterolemia. Cholesterol biosynthesis in the liver is influenced by the activity of the rate-limiting enzyme, HMG-CoA reductase. Traditional herbal medicine provides alternative treatment for hypercholesterolemia. Both pulai and papaya leaves were reported as HMG-CoA reductase inhibitors. There has been very limited study however, on the efficacy of a mixture of aqueous extracts of the two in inhibiting HMG-CoA reductase. This study aims to determine the activity of a mixture of water extracts of both pulai and papaya leaves. The activity of the enzyme HMG-CoA reductase was measured by Abcam® kit No. Ab204701 by spectrophotometric method at 334 nm. The results showed that the three extracts of the combination of pulai and papaya leaves had four times better inhibitory action than the single extract (p0.05). The results of the phytochemical literature study showed that the aqueous extract contained flavonoids, alkaloids, tannins, and saponins
Copyright (c) 2024 Sulistiyani, Dimas Andrianto, Sri Mariati
Minyak atsiri Kapulaga (Elettaria cardamomum) sebagai inhibitor Sap 5 Candida albicans penyebab kandidiasis vulvovaginalis (KVV) secara in silico
ABSTRACT Vulvovaginal candidiasis (VVC) is a disease caused by the inflammatory process of the vulva and vaginal mucosa caused by Candida sp., mainly Candida albicans. This study aimed to analyze the molecular interaction between the volatile oil in cardamom and Sap 5 as an inhibitor of Candida albicans causing VVC through In silico molecular interaction analysis. The methods used are analysis of homology, structural quality, and essential areas, receptor and ligand preparation, gridbox validation, virtual screening, Lipinski prediction and toxicity, and ligand-receptor interaction visualization analysis. The results showed that essential oils have the potential to inhibit Sap 5 through molecular bonding and produce interactions in the form of hydrogen bonds, electrostatic bonds, and hydrophobic interactions. The best test ligands were Geranyl acetate (-6.78 kcal/mol), Alpha-terpinyl acetate (-6.07 kcal/mol), 1,8-Sineol (-5.47 kcal/mol), and Linalool (-5.06 kcal/mol). The test ligands have contact with catalytic residues on Asp32/Asp218. In addition, the properties of these ligands also meet the Lipinski and Toxicity rules, so they can be predicted to be safe. Keywords: Candida albicans, Cardamom, Essential oil, In silico, Sap 5 ABSTRAK Kandidiasis vulvovaginalis (KVV) merupakan penyakit akibat dari proses inflamasi vulva dan mukosa vagina yang disebabkan oleh Candida sp. utamanya Candida albicans. Penelitian ini bertujuan untuk menganalisis interaksi molekuler antara minyak atsiri yang terkandung pada kapulaga dengan Sap 5 sebagai inhibitor Candida albicans penyebab KVV melalui analisis interaksi molekuler secara In silico. Metode yang digunakan yaitu analisis homologi, kualitas struktur, dan daerah penting, preparasi reseptor dan ligan, validasi gridbox, penapisan virtual, prediksi Lipinski dan toksisitas, dan analisis visualisasi interaksi ligan-reseptor. Hasil penelitian menunjukkan bahwa minyak atsiri mempunyai potensi menghambat Sap 5 melalui penambatan molekuler dan menghasilkan interaksi berupa ikatan hidrogen, ikatan elektrostatik, dan interaksi hidrofobik. Golongan ligan uji yang terbaik yaitu Geranil asetat (-6.78 kkal/mol), Alfa-terpinil asetat (-6.07 kkal/mol), 1,8-Sineol (-5.47 kkal/mol), dan Linalool (-5.06 kkal/mol). Ligan uji tersebut mempunyai kontak residu katalitik pada Asp32/Asp218. Selain itu, sifat ligan tersebut juga memenuhi aturan Lipinski dan Toksisitas, sehingga dapat diprediksi aman. Kata kunci: Candida albicans, Kapulaga, Minyak atsiri, In silico, Sap 5
Copyright (c) 2024 Gusnia Meilin Gholam, Rini Kurniasih, I Made Artika
INHIBISI ENZIM α-GLUKOSIDASE OLEH SENYAWA FLAVONOID DAUN KELOR (Moringa oleifera) IN SILICO DAN IN VITRO
Moringa leaves flavonoid have potential to inhibit α-glucosidase but they have low bioavailability, so they are made in nanoparticles. It is also not known which specific flavonoid compounds from Moringa leaves have the potential to inhibit α-glucosidase. This research aimed to determine the inhibition potential of α-glucosidase by the moringa leaves flavonoid in silico through molecular docking and determine the inhibitory activity of α-glucosidase by moringa leaves flavonoid in extracts and nanoparticles in vitro. Moringa leaves flavonoid have potential to be a competitive inhibiton of α-glucosidase with the highest to lowest inhibitory potential are cryptochlorogenic acid, quercetin-3-O-beta-D-glucopyranoside, quercetin-3-glucoside, kaempferol-3-O-a-ramnoside, kaempferol-3-O-glucoside, epicathechins, catechins, quercetin, kaempferol, glucomoringin isothiocyanate. Cryptochlorogenic acid has the best potential with ΔG and Ki values -8.5 kcal/mol and 0.5788 μM. Inhibition α-glucosidase moringa leaves flavonoid in extract and nanoparticles respectively are classified as inactive (IC 50 = 5.84x10 3 ppm) and active (IC 50 = 1.59x10 1 ppm) in vitro, so nanoparticles can increase the inhibitory activity.
Copyright (c) 2024 Puspa Julistia Puspita, Shobiroh Nuur Alimah, Laksmi Ambarsari, Riksa Nur Wahyuni
Peredaman Radikal DPPH oleh Minuman Mikroenkapsulasi dan Nanoenkapsulasi Sirih Merah (Piper crocatum)
Formulasi minuman fungsional ekstrak sirih merah dengan penambahan berbagai ekstrak rempah telah diteliti memiliki aktivitas antioksidan, namun hasil penerimaan konsumen masih rendah pada uji sensori karena rasanya pahit. Teknologi mikroenkpasulasi dan nanoenkapsulasi diketahui dapat melindungi komponen bioaktif dan dapat meningkatkan kualitas sensori pangan. Penelitian ini bertujuan untuk mengembangkan minuman fungsional berdasarkan ekstrak daun sirih merah menggunakan teknik mikroenkapsulasi dan nanoenkapsulasi dengan membandingkan ukuran partikel, kadar fenol, aktivitas antioksidan dan sensori dengan minuman ready to drink (RTD). Maltodekstrin 10% digunakan sebagai penyalut pada pembuatan minuman mikroenkapsulasi, sedangkan gum arab dan maltodekstrin digunakan untuk penyalut pada minuman nanoenkapsulasi. Ukuran partikel diuji menggunakan metode Particle Size Analyzer (PSA), total fenolik diuji dengan metode Folin- Ciocalteu, aktivitas antioksidan diuji dengan metode DPPH, dan evaluasi sensori diuji dengan tingkat hedonik (kesukaan). Hasilnya menunjukkan bahwa minuman fungsional minuman nanoenkapsulasi memiliki rata-rata ukuran partikel paling kecil yaitu sebesar 107,9 nm. Kandungan fenolik tertinggi pada minuman RTD sebesar (782,30 ± 2,54 mg GAE / g). Aktivitas antioksidan tertinggi terdapat dalam minuman mikroenkapsulasi yaitu (107,54 ± 0,83 μg / mL). Minuman fungsional nanoenkapsulasi memberikan tingkat hedonik (2,73 ± 1,19) yang lebih disukai dibandingkan minuman mikroenkapsulasi dan RTD. Teknik mikroenkapsulasi dapat meningkatkan aktivitas antioksidan dan teknik nanoenkapsulasi dapat menurunkan aktivitas antioksidan.
Copyright (c) 2024 Tiana Fitrilia Tiana, Mega Safithri Mega, Dinar Mutiara Hikmah Dinar
Peredaman Radikal Bebas DPPH dan Aktivitas Inhibisi α-Glukosidase oleh Ekstrak Air Jahe Merah
Red ginger (Zingiber officinale Roxb. var. Rubrum) contains phenolic compounds that have potential to reduce blood glucose levels (antihyperglycemic) in patients with diabetes mellitus. This study aimed to determine the inhibitory activity toward α-glucosidase, antioxidant activity in reducing DPPH free radicals, and total phenolic content of aqueous extract of red ginger. The red ginger was extracted using water solvent at 100 oC for 15 minutes. Analysis of antioxidant activity, inhibitory activity of α-glucosidase, and total phenolic content using the spectrofotometry. Antioxidant activity showed that the extracts from the first, second and third replicates were not significantly different (p<0.05). α-glucosidase inhibition activity showed that the 1st replicate extract had the highest value of 97.729% and was significantly different (p<0.05) from the 2nd and 3rd replicate extracts and acarbose. The total phenolic content showed that the 2nd replicate extract had the highest value, namely 27,624 mg GAE/g extract and was significantly different (p<0.05) from the 1st and 3rd replicate extracts. The 1st, 2nd, and 3rd replicates of red ginger aqueous extract has the same antioxidant activity and equivalent glucosidase inhibitory activity as acarbose.
Copyright (c) 2024 Mega Safithri, Syaefudin, Azka Adzkya Emalia Putri
Phytochemical Screening, Total Flavonoids and Antioxidants of Kalanchoe Pinnata Linn. Leaves
Kalanchoe pinnata Linn. or in Indonesia known as cocor bebek, this plants grow well in temperate and tropical regions. Cocor bebek is used to treat several diseases including skin diseases, wound healing, kidney stones, gallstones, diabetes. The pharmacological activities of cocor bebek leaves have been reported as anti-inflammatory, anti-oxidant and anti-microbial. The objective of this study is to determine the metabolite content of cocor bebek leaves, total flavonoid levels and antioxidant activity. Observations of secondary metabolites of cocor bebek leaves were carried out through qualitative secondary metabolite screening, while total flavonoid levels were carried out quantitatively UV-VIS with quercetin as a comparison, atioksidan activity through DPPH molecular inhibition testing. The results of this study indicate that cocor bebek leaves contain alkaloids, flavonoids, saponins, triterpenoids, tannins and phenols. 200gr of cocor bebek leaves obtained % yield extract is 14.63%. The total favonoid content obtained was 8.77% of quercetin, while the antioxidant inhibition of DPPH molecules obtained IC50 of 97.42 ppm.
Copyright (c) 2023 Eka Nurul Qomaliyah, Nurul Indriani, Atika Rohma, Ricka Islamiyati
Antibacterial Test of Endophytic Bacteria Isolated from Jawer Kotok (Coleus scutellarioides L. Benth) Against Bacillus cereus and Salmonella enteritidis
The use of synthetic antibiotics in small doses and long term against pathogenic bacteria can cause negative effects, such as resistance of pathogens and killing of good bacteria. We needs an innovation to substitute the use of synthetic antibiotics with natural antimicrobial compounds. Jawer kotok (Coleus scutellarioides L. Benth) has been used as medicinal plant and shown to be efficacious as antimicrobial because it contains flavonoids, tannins, alkaloids and saponins. Endophytic bacteria are symbiotic microorganisms that live in healthy plant tissue without harming to the host plant and may produce natural antimicrobial compounds which similar to the host plant. The aim of this study are to isolating endophytic bacteria from jawer kotok and screening their antibacterial activity against two pathogenic bacteria, namely Bacillus cereus and Salmonella enteritidis. A total of 22 bacterial isolates were obtained from C scutellarioides [L.] Benth. Based on antibacterial activity test, nine isolates shown antibacterial activity against B. cereus and S.enteritidis. BJ7 is the most potential isolate because it shown the biggest inhibition. It great possible that endophytic bacteria from jawer kotok may synthesize the same antimicrobial compounds like their host plant.
Copyright (c) 2023 Dwi Endah Kusumawati, Maria Bintang
In Vitro Analysis of Gradual Water Extract of Red Betel Leaf (Piper crocatum) as Free Radical Scavenging and Inhibitor of α-Glucosidase
Red betel (Piper crocatum) has antihyperglycemic activity for diabetic treatment. The purpose of this study was to investigate the activity of DPPH free radical scavenging, the α-glucosidase inhibitory activity, and total phenolics of the aqueous extract of P. crocatum (1st extract, 1st extract residue, and 2nd extract residue). The infundation method was used with spectrophotometric test techniques. The red betel leaves extract in all extracts and extract residues had a small of α-glucosidase inhibition activity ranging from 0.76% - 1.43%. Meanwhile, the antioxidant activity of DPPH and total phenolic levels showed significantly different results (p<0.05) in the 1st extract, 1st extract residue, and 2nd extract residue. The highest antioxidant capacity and total phenolic content were found in the first extract, it were 59.64 ± 0.18 mg AAE/g extract and 55.50 ± 0.14 mg GAE/g, respectively. Gradual extraction reduced DPPH free radical scavenging activity, α-glucosidase inhibition activity and phenolic total content.
Copyright (c) 2024 Mega Safithri, Tiara Fibri Yuniasih, Syaefudin
A Brief Overview and History of Human Nutrition and Health
Nutritional biochemistry is finally getting the attention it deserves, despite the fact that food and nutrition have been studied for centuries. In 1926, less than 100 years ago, the first vitamin was extracted and chemically characterized, leading to studies on single-nutrient deficiency diseases. Nutritional research on chronic diseases like cardiovascular disease, diabetes, obesity, and malignancies has hastened in the past few decades, especially after the year 2000. Nutrition has played a key role in the improvement of public health since the past 200 years, and is expected to play a major role in public health for the future. Keywords: nutrition, diet, history, food, agriculture.
A Novel Plaster Dressing: A Combination of Chicken Eggshell Hydrogel Plus Button-Fungus Extract Enhances Wound Healing in Diabetic Patients
Diabetic patients are susceptible to severe wound conditions due to high blood sugar levels and bacterial infections. This study aims to determine the formulation and effectiveness of the hydrogel plaster combination of laying hen eggshells with button mushrooms in curing diabetic wounds. The research methods consisted of making the hydrogel, coagulation test, and antibacterial tests. Hydrogels were made with 5%, 10%, and 20% eggshell concentrations. The coagulation activity test used rabbit blood, which was added with glucose. Antibacterial test was performed using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This research shows that the coagulation activity of F2 and F3 formulations significantly differed from the control, with an average blood clotting time of 64.67 minutes and 63.00 minutes. The antibacterial test results produced a clear zone for S. aureus and E. coli with diameters of 10.27 mm and 9.18 mm for F2, 6.90 mm, and 11.66 mm for F3. This research concludes that hydrogel formulation 3, with 20% eggshell and 6.4% button mushroom, had the best blood clotting effect and antibacterial activity.
Copyright (c) 2024 Akbar Rafiqi, Alivianisa Nabilatusyifa, Oyi Candra Santoso Putri, Ikromatul Hasanah, Sabrina Firdayani Rahmah, Dimas Andrianto
Molecular docking: Bioactive compounds of Mimosa pudica as an inhibitor of Candida albicans Sap 3
Candida albicans (C. albicans) is a commensal microbiota that resides in humans. However, in certain cases, C. albicans can infect and cause several diseases to humans. This study aimed to investigate the interaction between Mimosa pudica bioactive compounds and C. albicans Sap 3. Molecular docking analysis was carried out using YASARA structure. The procedures involved preparation of ligands and target receptor, molecular docking, data analysis and visualization. All 3D ligands were downloaded from PubChem NCBI, while target receptor was downloaded from RCSB PDB. The interaction between Mimosa pudica bioactive compounds against Sap 3 resulted in a binding energies ranges from 5,168 – 7,480 kcal/mol and most of the interactions formed were relatively strong. Furthermore, the test ligands had contact with the catalytic residues and substrate binding site pockets S1/S2/S3/S4 on the target receptor. Bioactive compounds of Mimosa pudica have relatively good interactions in inhibiting C. albicans Sap 3
Copyright (c) 2023 Gusnia Meilin Gholam, Iman Akhyar Firdausy, I Made Artika, Ramadhani Malik Abdillah, Ridwan Putra Firmansyah, Riyan Alifbi Putera Irsal, Hafizh Zahra
Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae
Antibacterial resistance in Streptococcus pneumoniae has been increasing and is one of ongoing global concern. The need to find new antibacterial agents against Streptococcus pneumoniae is of paramount importance. Medicinal plants are prospective sources of antibacterial agents. The aims of the present study were to determine the activity of leaf extraxt of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. against Streptococcus pneumoniae. Leaves of Anredera cordifolia (Ten.) Steenis were extracted using 96% ethanol, while the leaves of Muntingia calabura L were extracted using 100% methanol. The leaf extracts of the two plants obtained were bioassayed for antibacterial activity against Streptococcus pneumoniae ATCC 49619 and a clinical isolate Streptococcus pneumoniae PU 067. Results showed that leaf extracts of both Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. have antibacterial activity in vitro against Streptococcus pneumoniae ATCC 49619 at crude extract concentrations of 25%, 50%, 75% and 100% (w/v). Both plants extracts showed strongest activity against S. pneumoniae ATCC 49619 at extract concentration of 75%. In addition, the extracts of both plants have inhibitory activity against growth of the clinical isolate Streptococcus pneumoniae PU 067. Both plant extracts showed strongest activity against S. pneumoniae PU 067 at extract concentration of 100%. Therefore, leaf extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. can potentially be used as a source of antibacterial agent for Streptococcus pneumoniae. Keywords: Antibacterial agent, Anredera cordifolia (Ten.) Steenis, Muntingia calabura L., Streptococcus pneumoniae.
Antibacterial Activity of Ethanol Extract of Spirulina platensis
Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.
Copyright (c) 2020 Prayoga Pannindrya, Mega Safithri, Kustiariyah Tarman
Differences in Parts of Purslane Plants (Portulaca grandiflora Hook.) To Total Phenolic and Flavonoid Content and Antioxidant Activity
Antioxidant are needed to prevent free radical inside the body and oxidative stress that promotes the aging process and various degenerative diseases. Portulaca grandiflora Hook. is known to have potential as antioxidant. Differences in plant parts can cause differences in phytochemical content, which can affect its antioxidant activity. This study aims to measure total phenolic and flavonoid content in leaf, old stem, young stem, and flower organs of purslane plant (Portulaca grandiflora Hook.) which is extracted using ethanol 96% and determined the antioxidant activity of the extract with DPPH methode. The result showed that each plant organ of purslane contains different concentration of phenolic and flavonoid compounds. The highest total phenolic and flavonoid content was found in the leaves( 113.26 ± 3.85 mg GAE/g and 97.99 ± 1.28 mg QE/g), but the highest antioxidant activity was found in the old stem with IC50 value 122.15 ± 1.30 ppm (classified as medium antioxidant). Keywords: Antioxidant, Flavonoid, Phenolic, Portulaca grandiflora Hook.
Minimum Concentration of Stingless Bee Propolis (Trigona sp.) in Inhibiting the Growth of Dandruff Bacteria
Lack of confidence in men and women, one of which is when there is dandruff on the hair and scalp. Hair loss is a further symptom of the presence of white scales on the scalp that cause itching. The presence of excessive microorganisms can aggravate this dandruff. Ingredients such as ketocanozole, zinc pyrithione, selenium sulfide and sulfur are compounds commonly used in shampoo formulas. Propolis is an alternative natural ingredient to treat dandruff. The purpose of this study was to determine the minimum growth inhibitory concentration (MIC) of propolis against dandruff-causing bacteria. Dandruff-causing microbes were isolated from dandruff hair and with the well method in a petri dish, the minimum growth inhibitory concentration of propolis was determined. The microbial colonies produced were very diverse with a round shape like grapes, yellowish white in color. The concentration of 6.25% is the MIC value of propolis extract against dandruff microbes. The effectiveness values against ketoconazole and propolis X from the tested propolis extract were 223.52% and 99.59%, respectively. Keywords: hair loss, dandruff, propolis, bacteria , Trigona
Copyright (c) 2021 Akhmad Endang Zainal Hasan, I Made Artika, Erlank Bagjavicenna
Antioxidant Activities and α-glucosidase Inhibition of Gayo Arabica Coffee Skin (Coffea arabica L)
This research objectives to determine antioxidant activities and inhibition of α-glucosidase 96 % ethanol extract of gayo arabica coffee skin, n-hexane fraction, ethyl acetate fraction, and water fraction. Gayo arabica coffee skin was extracted by ethanol using maceration and fractionation using n-hexane, ethyl acetate, and water. Quercetin and Gallic acid were used as standard to found total flavonoids and phenolics. Test of antioxidant activity using the DPPH method. After being tested the inhibition of the α-glucosidase IC50 ethanol extract, the n-hexane fraction, ethyl acetate fraction, and water fractions. The total flavonoids of 96% ethanol extract, n-hexane fraction, ethyl acetate, and water fraction respectively are 76.74, 99.07, 73.55, 50.24 mg QE/g extract . Total phenolics of 96% ethanol extract, n-hexane fraction, ethyl acetate, and water fraction respectively are respectively 211.85, 136.67, 145.19, 127.41 mg GAE/ g extract. Antioxidants activity of ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction are IC50 12.03 ppm, 36.08 ppm, 30.27 ppm, 11.62 ppm. Inhibition activity α-glucosidase enzyme respectively are 588.85 ppm, 341.81 ppm, 591.61 ppm, and 798.78 ppm. These showed that the ethanol extract and water fraction are the best in antioxidants and the ethanol extract and ethyl acetate fraction are the best in inhibiting the α-glucosidase enzyme. These results indicate that gayo arabica coffee skin extract has the potential to be developed as an antidiabetic compound. Keywords: Antidiabetic, Antioxidant, flavonoids, phenolics, α-glucosidase
Copyright (c) 2021 Baharuddin Yusuf Habiby Harahap, Hasim Hasim, Didah Nur Faridah
Antioxidant Activity, Inhibition α-Glucosidase of Cymbopogon nardus (L.) Rendle and Identification of Active Compounds
Diabetes mellitus (DM) has become a health problem in various countries, even in Indonesia, multiple of Covid-19 sufferers have DM as comorbid. Treatment of DM can be conducted by delaying glucose intake performed by the α-glucosidase enzyme in the intestine. Treatment using synthetic drugs often causes side effects. Instead to overcome these problems, herbal medicine is a choice. Cymbopogon nardus (L.) Rendle served as a potential candidate as seen from prior research but related to DM drugs, further exploration needs to be performed. This study aims to acquire a DM medicine by using Cymbopogon nardus (L.) Rendle . The leaves were macerated using 96% concentration of ethanol followed by multilevel fractionation with n-hexane, water and ethyl acetate as solvents. The test results showed that the n-hexane fraction was the best for antioxidant activity and α -glucosidase inhibition with IC50 values of 8,23 ppm and 311,87 ppm while the identification of the compound by using Liquid Chromatograph-Mass Spectrometry (LC-MS/MS) suspected compounds that have a role were ar-turmerone, beesioside N and notohamosin A. Based on the test, the n-hexane fraction was found as a source of antioxidants as well as α -glucosidase inhibitors. Keywords: Antioxidant, α -glucosidase, Cymbopogon nardus (L.) Rendle, LC-MS/MS.
Copyright (c) 2021 Najmah Najmah, Hasim Hasim, Didah Nur Faridah
Review: Gallotannins; Biosynthesis, Structure Activity Relationship, Anti-inflammatory and Antibacterial Activity
Tannins are one of the main compounds in plants with potential health benefits. Gallotannin is one of the biologically active tannins groups produced by some medicinal plants. Gallotannins is a hydrolyzable tannin compound biosynthesized through gallic acid, shikimic acid, and pentagalloylglucose pathways. Gallotannins can be separated by cascade solvent extraction procedures, column chromatography, and preparative HPLC. This review focuses on the discussion of biosynthesis and structure-activity relationship of tannins as antibacterial and anti-inflammatory. The structure-activity relationship (SAR) and biological activity of gallotannins is caused by the molecular bonds between gallotannins and certain other compounds, especially proteins, which make complex compounds, change physiological and morphological processes in bacterial cells or tissues. The biological activities of gallotannin include anti-inflammatory, anticancer or antitumour, antiviral, antioxidant, antibacterial, and antidiabetic. This review used the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) method approach in a short, concise, and clear summary about tannins through various references. Keywords: Biological activities, Biosynthesis, Gallotannins
Copyright (c) 2021 Ilham Kurniawan, Hafizh Zahra