Proximate Characteristics and Antioxidant Activity of Polyherbal Extract in Yogurt
DOI:
https://doi.org/10.18343/jipi.30.4.638Abstract
Fermented dairy products, such as yogurt, are well known for their health advantages. Enhancing their functional value using polyherbal mixtures constitutes a big step forward in functional food development. This study looks at the proximate composition (fat, protein, moisture, and ash content) and antioxidant activity of polyherbal yogurt enriched with green tea, cinnamon, and fenugreek seed extracts. Formulations were created with varied amounts of polyherbal extract at 0% (P0), 1% (P1), 2% (P2), and 3% (P3), with Lactobacillus bulgaricus and Streptococcus thermophilus as starter cultures (Lactina Ltd). A completely randomized design with three replications was used, and the data were analyzed using ANOVA at a 95% confidence level. The results showed substantial variations in fat (2.84−3.62%) and moisture (88.17−8.83%) content between treatments. Protein (3.92−5.03%) and ash level (0.76−0.77%) met the Indonesian National Standard (SNI) for yogurt. Polyherbal yogurt demonstrated considerably higher antioxidant activity and capacity than the control. The control yogurt (P0) had a poor antioxidant capacity of 60.81±10.89%, while polyherbal yogurt (P1, P2, and P3) had a high antioxidant capacity ranging from 252.40±11.20% to 267.44±1.44%. These findings indicate that including polyherbal extracts into yogurt not only ensures compliance with national nutritional standards (SNI), but also considerably increases antioxidant potential. Thus, polyherbal yogurt is an appealing prospect for development as a beneficial dairy product.
Keywords: antioxidant, polyherbal, proximate, yogurt
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References
[AOAC] Association of Official Analytical Chemistry. 2005. Official Methods of Analysis of AOAC Internasional. 18th ed. Maryland (US): Association of Analytical Chemistry
Adler P, Bolten CJ, Dohnt K, Hansen CE, Wittmann C. 2013. Core fluxome and metafluxome of lactic acid bacteria under simulated cocoa pulp fermentation conditions. Applied and Environmental Microbiology. 79(18): 5670–5681. https://doi.org/10.1128/AEM.01483-13
Akgun A, Yazici F, Gulec HA. 2016. Effect of reduced fat content on the physicochemical and microbiological properties of buffalo milk yoghurt. Food Science and Technology. 74: 521–527. https://doi.org/10.1016/j.lwt.2016.08.015
Al–Shawi SG, Ali HI, Al–Younis ZK. 2020. The effect of adding thyme extracts on microbiological, chemical, and sensory characteristics of yogurt. Journal of Pure Applied Microbiology. 14(2): 1367–1376. https://doi.org/10.22207/JPAM.14.2.34
Anjasari IRD. 2016. Katekin teh Indonesia: Prospek dan manfaatnya. Indonesia tea catechin: Prospect and benefits. Jurnal Kultivasi. 15(2): 99–106. https://doi.org/10.24198/kultivasi.v15i2.11871
Ayar A, Gürlin E. 2014. Production and sensory, textural, physicochemical properties of flavored spreadable yogurt. Life Science Journal. 11(4): 58–65.
Cheigh HS, Park KY, Lee C. 1994. Biochemical, microbiological, and nutritional aspects of kimchi (Korean fermented vegetable products). Critical Reviews in Food Science and Nutrition. 34(2): 175–203. https://doi.org/10.1080/10408399409527656
Dhawi F, El–Beltagi HS, Aly E, Hamed AM. 2020. Antioxidant, antibacterial activities and mineral content of buffalo yogurt fortified with klabet and Moringa oleifera seed flours. Foods. 9: 1–16. https://doi.org/10.3390/foods9091157
Domagala J, Wszolek M, Tamime AY, Kupiec–Teahan B. 2013. The effect of transglutaminase concentration on the texture, syneresis and microstructure of set–type goat's milk yoghurt during the storage period. Small Ruminant Research. 112(1): 154–161. https://doi.org/10.1016/j.smallrumres.2012.12.003
Emilia I, Setiawan AA, Novianti D, Mutiara D, Rangga. 2023. Skrining fitokimia ekstrak daun sungkai (Peronema canescens Jack.) secara infundasi dan maserasi. Jurnal Indobiosains. 5(2): 95–102. https://doi.org/10.31851/indobiosains.v5i2.12597
Estiasih T, Ahmadi K. 2016. Teknologi Pengolahan Pangan. Jakarta (ID): Rajawali Press.
Gemede H, Haki G, Beyene F, Woldegiorgis A, Rakshit S. 2015. Proximate, mineral, and antinutrient compositions of indigenous Okra (Abelmoschus esculentus) pod accessions: Implications for mineral bioavailability. Food Science & Nutrition. 4: 223–233. https://doi.org/10.1002/fsn3.282
Gharibzahedi SMT, Chronakis IS. 2018. Crosslinking of milk proteins by microbial transglutaminase: utilization in functional yogurt products. Food Chemistry. 245: 620–632. https://doi.org/10.1016/j.foodchem.2017.10.138
He Y, Ding C, Wang X, Wang H, Suo Y. 2015. Using response surface methodology to optimize countercurrent chromatographic separation of polyphenol compounds from klabet (Trigonella foenum–graecum L.) seeds. Journal of Liquid Chromatography & Related Technologies. 38: 29–35. https://doi.org/10.1080/10826076.2013.864981
Hidayah H, Zulfa AN, Nurjanah A, Septanti R, Nadeak ZT. 2024. Perbandingan kadar antioksidan pada tumbuhan jamblang dengan metode DPPH, FRAP, dan ABTS. Journal of Social Science Research. 4(1): 3359–3373.
Hussain A, Bose S, Wang JH, Yadav MK, Mahajan GB, Hojun K. 2016. Fermentation, a feasible strategy for enhancing bioactivity of herbal medicines. Food Research International. 81: 1–16. https://doi.org/10.1016/j.foodres.2015.12.026
Jeki MW, Zulfanita, Dedi R. 2019. The antioxidant activity of yogurt drink by mangosteen rind extract (Garcinia mangostana L.). Journal of Applied Food Technology. 6(1): 15–18. https://doi.org/10.17728/jaft.4267
Kadhka M. 2023. Effect of frying time on phytochemical and antioxidant activity of fenugreek seeds (Trigonellafoenum–graecum) and cumin seeds (Cuminum cyminum) [Doctoral dissertation]. Nepal (NP): Tribhuvan University.
Kamiya H, Shinozaki H, Ymamoto C. 1996. Activation of metabotropic glutamate receptor type 2/3 suppresses transmission at rat hippocampal mossy fiber synapses. Journal of Physiology. 493(2): 447–455. https://doi.org/10.1113/jphysiol.1996.sp021395
Kumalasari ID, Fathita LN, Septiyani R. 2024. Physicochemical, microbiological and organoleptic properties of cowpeas (Vigna unguiculata) yoghurt with the addition of gotu kola leaf (Centella asiatica (l.) Urban) extract. Sains Malaysiana. 53(2): 123–134. https://doi.org/10.17576/jsm-2024-5301-10
Manzanares CG, Beltran LM, Hernández A, González AF, Vallejo B. 2021. Invited review: Potential antiobesity effect of fermented dairy products. Journal of Dairy Science. 104(4): 3766–3778. https://doi.org/10.3168/jds.2020-19256
Mohamed AF, Haidy AS, Sherwet A, Moamen ME. 2021. Dissecting yogurt: the impact of milk types, probiotics, and selected additives on yogurt quality. Food Reviews International. 10: 1–18.
Muniandy P, Shori AB, Baba AS. 2016. Influence of green, white and black tea addition on the antioxidant activity of probiotic yogurt during refrigerated storage. Food Packaging and Shelf Life. 8: 1–8. https://doi.org/10.1016/j.fpsl.2016.02.002
Nugroho MR, Wanniatie V, Qisthon A, Septinova D. 2023. Sifat fisik dan total bakteri asam laktat (BAL) yoghurt dengan bahan baku susu sapi yang berbeda. Jurnal Risetdan Inovasi Peternakan. 7(2): 279–286. https://doi.org/10.23960/jrip.2023.7.2.279-286
Pazra DF, Purwanti M, Handayani KS, Wahyuningsih, Wahyuwardani S. 2023. Antidiabetic and hypolipidemic potential of cow’s milk yogurt with the addition of herbal extracts in diabetic rats. Tropical Animal Science Journal. 46(4) 494–501. https://doi.org/10.5398/tasj.2023.46.4.494
Putri WCW, Yuliawati, Rahman H. 2021. Uji aktivitas hepatoprotektor ekstrak etanol daun rambutan (Nephelium lappaceum L.) pada mencit putih jantan yang diindukasi parasetamol. Pharmacon. 18(2): 148–156. https://doi.org/10.23917/pharmacon.v18i2.15732
Rahadian D, Muhammad A, Dewettinck K. 2017. Cinnamon and its derivatives as potential ingredient in functional food: A review. International Journal of Food Properties. 20(2): 2237–2263.
Ratnani S, Malik S. 2022. Therapeutic properties of green tea: A Review. Journal of Multidisciplinary Applied Natural Science. 2(2): 90–102. https://doi.org/10.47352/jmans.2774-3047.117
Rong T, Li H. 2012. Antioxidant properties in vitro and in vivo: realistic assessments of efficacy of plant extracts. CABI Reviews. 7(9): 1-9. https://doi.org/10.1079/PAVSNNR20127009
Scalbert A, Manach C, Morand C, Rémésy C. 2005. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition. 45:287–306. https://doi.org/10.1080/1040869059096
Shori AB, Baba AS, Misran M, Tan HW. 2013. Enrichment of yogurt made from camel milk with Allium sativum and Cinnamomum verum: Influence on syneresis, water holding capacity, expolysaccharides and rheological properties. International Journal of Veterinary Sciences. 1(1): 51–63.
[SNI] Standardisasi Nasional Indonesia. 2009. SNI Yogurt 01–2981–2009. Jakarta (ID): Badan Standardisasi Nasional.
Soni R, Jain NK, Shah V, Soni J, Suthar D, Gohel P. 2020. Development of probiotic yogurt: effect of strain combination on nutritional, rheological, organoleptic and probiotic properties. Journal of Food Science and Technology. 57: 2038–2050. https://doi.org/10.1007/s13197-020-04238-3
Stanton C, Ross RP, Fitzgerald GF, Sinderen DV. 2005. Fermented functional foods based on probiotics and their biogenic metabolites. Current Opinion in Biotechnology. 16(2): 198–203.
Steel R, Torrie JK. 1997. Prinsip dan Prosedur Statistik., Jakarta (D): Gramedia Pustaka Utama.
Tangkanakul P, Auttaviboonkul P, Niyomwit B, Lowvitoon N, Charoenthamawat P, Trakoontivakorn G. 2009. Antioxidant capacity, total phenolic content 17 and nutritional composition of Asian foods after thermal processing. Journal International Food Research. 16: 571–580.
Wahyuningsih, Pazra DF. 2022. Kualitas sfisik, kimia, mikrobiologi susu sapi pada peternakan sapi perah di Kecamatan Caringin, Kabupaten Bogor. Jurnal Agroekoteknologi dan Agribisnis. 6(1): 1–16. https://doi.org/10.51852/jaa.v6i1.532
Wihansah RRS, Pazra DF, Handayani KS. 2022. Assesment of the Antidiabetic Activity and Characteristics of Cow’s Milk Yogurt Enhanced with Herbs Extracts. In IOP Conference Series: Earth and Environmental Science; 10–12 Nov. 2021; pp. 1–10. https://doi.org/10.1088/1755-1315/1020/1/012024
Xing L, Zhang H, Qi R, Tsao R, Mine Y. 2019. Recent advances in the understanding of the health benefits and molecular mechanisms associated with green tea polyphenols. Journal of Agricultural and Food Chemistry. 67(4): 1-15. https://doi.org/10.1021/acs.jafc.8b06146
Yan Z, Zhong Y, Duan Y, Chen Q, Li F. 2020. Antioxidant mechanism of tea polyphenols and its impact on health benefits. Animal Nutrition. 6(2): 115-123. https://doi.org/10.1016/j.aninu.2020.01.001
Yiannakopoulou EC. 2013. Targeting oxidative stress response by green tea polyphenols: clinical implications. Free Radical Research. 47: 667-671. https://doi.org/10.3109/10715762.2013.819975
Zain WNH, Mirdhayati I. 2021. Pengaruh penambahan minyak zaitun pada yogurt susu kambing terhadap profil asam lemak, kolesterol, pH dan jumlah bakteri asam laktat. Warta Industri Hasil Pertanian. 38(1): 9–16. https://doi.org/10.32765/wartaihp.v38i1.6377
Zhao Y, Khalesi H, He J, Fang Y. 2023. Application of different hydrocolloids as fat replacer in low–fat dairy products: ice cream, yogurt and cheese. Food Hydrocoloids. 138: 1–13. https://doi.org/10.1016/j.foodhyd.2023.108493
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