Repellent and Metamorphosis Capability of Alphitobius diaperinus Exposed to Gamal Leaf Extracts
DOI:
https://doi.org/10.18343/jipi.30.3.517Abstract
Alphitobius diaperinus is an insect pest that is difficult to control in conventional chicken farms. In high populations, this insect can damage the infrastructure of the coop, disrupt chicken feeding activities, and is a known vector of pathogens. Currently, the control of A. diaperinus still relies on synthetic insecticides that have been proven to be resistant to target pests, toxic to non-target organisms, and pollute the environment. This study aimed to analyze the potential of gamal (Gliricidia sepium) leaf extract as a bioinsecticide in terms of repellency response and its effect on the metamorphosis of A. diaperinus. The repellency response was measured through a preference test using a Y-olfactometer at 6 concentration levels, whereas metamorphosis ability was tested at 6 sub-lethal concentrations. Each treatment was performed in 5 replications. The repellency response was descriptively analyzed based on the repellency percentage (PP) and preference index (IP) values. The metamorphosis ability test results were analyzed using non-parametric Kruskal-Wallis and Mann-Whitney U tests. The results showed that all treatments had PP values > 80% with negative IP. The results of the Kruskal-Wallis test showed significant differences in the extract concentrations between the treatment groups (asymp. sig < 0.05). A higher extract concentration caused a decrease in the number of larvae that successfully became imagoes. In conclusion, A. diaperinus showed a high repellency response to gamal leaf extract, and the gamal leaf extract had a strong repellent effect. The extract concentration also affected the metamorphosis of A. diaperinus larvae.
Keywords: Alphitobius diaperinus, bioinsecticide, Gliricidia sepium, Metamorphosis, Repellency response
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References
Chaubey MK. 2018. Study of insecticidal properties of Trachyspermum ammi and Mentha arvensis essential oils against Sitophilus zeamais L. (Coleoptera: Curculionidae). Current Life Sciences. 4: 10–17.
Chopra B, Dhingra AK, Kapoor RP, Prasad DN. 2016. Piperine and Its various physicochemical and biological aspects: A Review. Chemistry Journal. 3: 75–96. https://doi.org/10.2174/1874842201603010075
Das N, Mishra SK, Bishayee A, Ali ES, Bishayee A. 2021. The phytochemical, biological, and medicinal attributes of phytoecdysteroids: An updated review. Acta Pharmaceutica Sinica B. 11: 1740–1766. https://doi.org/10.1016/j.apsb.2020.10.012
Das N, Mishra SK, Bishayee A, Ali ES, Bishayee A. 2021. The phytochemical, biological, and medicinal attributes of phytoecdysteroids: An updated review. Acta Pharmaceutica Sinica B. 11: 1740–1766. https://doi.org/10.1016/j.apsb.2020.10.012.
Esquivel JF, Crippen TL, Ward LA. 2012. Improved visualization of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)-Part I: Morphological features for sex determination of multiple stadia. Psyche. 1–7. https://doi.org/10.1155/2012/328478
Indriyani YA. 2019. Pergerakan pestisida organofosfat di dalam ekosistem. Agrikultur [Internet]. [accessed on 2024 Jun 05]: 1–14. Available on https://www.researchgate.net/publication/342492781
Jiang X, Hansen HCB, Strobel BW, Cedergreen N. 2018. What is the aquatic toxicity of saponin-rich plant extracts used as biopesticides. Environmental Pollution. 236: 416–424. https://doi.org/10.1016/j.envpol.2018.01.058
Jing X, Grebenok RJ, Behmer ST. 2012. Plant sterols and host plant suitability for generalist and specialist caterpillars. Journal of Insect Physiology. 58: 235–244. https://doi.org/10.1016/j.jinsphys.2011.11.013
Jose S, Sujatha K. 2017. Antifeedant activity of different solvent extract of GLiricidia sepium againts third instar larvae of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). Advanced Research in Biological Sciences. 4: 201–204. https://doi.org/10.22192/ijarbs.2017.04.04.026
Kartini A, Tarihan D, Saleh C. 2017. The phytochemical test and toxicity test leaves gamal (Gliricidia sepium) extract as a botanical insecticide. Kimia Mulawarman [Internet], [accessed 2024 Jun 05]: 15: 53–59. Tersedia pada https://jurnal.kimia.fmipa.unmul.ac.id/index.php/jkm/article/view/399 https://doi.org/10.30872/jkm.v15i1.399
Kasman K, Ishak NI, Hastutiek P, Suprihati E, Mallogi A. 2020. Identification of active compounds of ethanol extract of Citrus amblycarpa leaves by analysis of thin-layer chromatography and gas chromatography-mass spectrometry. Macedonian Journal of Medical Sciences. 8: 1–6. https://doi.org/https://doi.org/10.3889/oamjms.2020.5207
Khumaira F. 2021. Pestisida nabati ekstrak daun gamal (Gliricidia sepium Jacq. Kunth) terhadap ulat daun (Spodoptera exigua Hubner) tanaman bawang merah. [Undergraduate thesis]. Semarang (ID): Universitas Islam Negeri Walisongo
Kumara CJ, Nurhayani, Bestari RS, Dewi LM. 2021. Efektivitas flavonoid, tanin, saponin dan alkaloid terhadap mortalitas larva Aedes aegypty. In WY Adam, AN Nur, K Dwi (Eds.). In: Prosiding Kesehatan dan MIPA. Klaten, Jawa Tengah, 20 Mar 2021.
Kurniawan N, Yulianti, Rachmadiarti F. 2013. Uji bioaktivitas ekstrak daun suren (Toona sinensis) terhadap mortalitas larva Plutella xylostella pada tanaman sawi hijau. LenteraBio: Berkala Ilmiah Biologi. 2: 203–206. https://core.ac.uk/download/pdf/230675222.pdf
Lyons BN. 2021. The lesser mealworm (Alphitobius diaperinus) association with enterococcus cecorum and pest management considerations for this pest in broiler houses. Entomolgy. 2: 1–136. https://doi.org/10.1111/raq.12300
Maazoun AM, Hamdi SH, Belhadj F, Jemâa JMB, Messaoud C, Marzouki MN. 2019. Phytochemical profile and insecticidal activity of Agave americana leaf extract towards Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Environmental Science and Pollution Research. 26: 19468–19480. https://doi.org/10.1007/s11356-019-05316-6
Marawali HH. 2017. Teknologi pakan sebagai alternatif perbaikan produktivitas sapi sumba Ongole di Pulau Sumba. In: Prosiding Seminar Nasional. Ambon, Maluku, Oct 12–13, 2016
Mohammad MY, Haniffa HM, Shakya AK, Naik RR, Sivaranjan T. 2024. Evaluation of five medicinal plants for the management of Sitophilus oryzae in stored rice and identification of insecticidal compound. Heliyon. 10: 1–9. https://doi.org/10.1016/j.heliyon.2024.e30793
Muta R, Indah K. 2015. Pengaruh ekstrak daun beluntas (Pluchea indica) terhadap mortalitas dan perkembangan larva Spodoptera litura F. Jurnal Sains Dan Seni ITS. 4: 2–5.
Nguyen N, Yang B-K, Lee J-S, Yoon J-U, Hong K-J. 2019. Infestation status of the darkling beetle (Alphitobius diaperinus) in broiler chicken houses of Korea. Korean Journal of Applied Entomology. 58: 2287–2545.
Nukmal N, Pratami GD, Rosa E, Sari A, Kanedi M. 2019. Insecticidal effect of leaf extract of gamal (Gliricidia sepium) from different cultivars on papaya mealybugs (Paracoccus marginatus, Hemiptera: Pseudococcidae). International Organization of Scientific Research Journal of Agriculture and Veterinary Science. 12: 04–08.
Nurhudiman N, Hasibuan R, Hariri AM, Purnomo P. 2018. Uji potensi daun babadotan (Ageratum conyzoides L.) sebagai insektisida botani terhadap hama (Plutella xylostella L.) di laboratorium. Jurnal Agrotek Tropika. 6: 91–98. https://doi.org/10.23960/jat.v6i2.2600
Pratiwi BYH, Muhsin LB. 2023. Effect of gamal leaves (Gliricidia sepium) extract against termite (Coptotermes curvignathus). Jurnal Pijar Mipa. 18: 970–975. https://doi.org/10.29303/jpm.v18i6.5863
Ramayanti I, Layal K, Pratiwi PU. 2017. Effectiveness test of basil leaf (Ocimum basilicum) extract as bioinsecticide in mosquito coil to mosquito Aedes aegypti death. Journal of Agromedicine and Medical Sciences. 3: 6. https://doi.org/10.19184/ams.v3i2.5063
Rinaldi FB, Rachmawati J, Udiarto BK. 2016. Pengaruh ekstrak bunga krisan (Chrysanthemum cinerariaefolium Trev.), bunga saliara (Lantana camara Linn.) dan bunga lavender (Lavandula angustifolia Mill.) terhadap repellency kutu kebul (Bemisia tabaci Genn.). Bioed: Jurnal Pendidikan Biologi. 4: 42–49. https://doi.org/10.25157/jpb.v4i1.554
Roopashree KM, Naik D. 2019. Saponins : Properties, applications and as insecticides: A Review. Trends in Biosciences [Internet]: [accessed on 2024 Jun 02]: 12. 1–14. Available on https://www.researchgate.net/publication/333531471
Sari AO, Isworo S. 2020. The potential biopesticide toxicity test of Ipomoea batatas (L.) Lam (purple sweet potato leaf extract) against Artemia salina leach larvae using the brine shrimp lethality test method. International Journal of Scientific and Research Publications. 10: 212–217. https://doi.org/10.29322/ijsrp.10.08.2020.p10428
Sharma N, Palia P, Chaudhary A, Shalini, Verma K, Kumar I. 2020. A Review on pharmacological activities of lupeol and its triterpene derivatives. Journal of Drug Delivery and Therapeutics. 10: 325–332. https://doi.org/10.22270/jddt.v10i5.4280
Subahar R, Aulung A, Husna I, Winita R. 2020. Effects of Lansium domesticum leaf extract on mortality, morphology, and histopathology of Aedes aegypti larvae (Diptera: Culicidae). International Journal of Mosquito Research. 7: 105–111. https://www.dipterajournal.com/pdf/2020/vol7issue4/PartB/7-4-6-606.pdf
Subekti N, Cahyaningrum SH, Maulana S. 2022. Effective control of Alphitobius diaperinus using natural bioinsecticides. Journal of Tropical Life Science. 12: 289–297. https://doi.org/10.11594/jtls.12.03.01
Sultan A, Raza AR. 2015. Steroids: A diverse class of secondary metabolites. Medicinal Chemistry. 5: 310–317. https://doi.org/10.4172/2161-0444.1000279
Sunarno S, Nagari AP. 2022. Efek dinamika faktor lingkungan terhadap perilaku ayam broiler di kandang close house. Jurnal Peternakan Indonesia. 24: 8. https://doi.org/10.25077/jpi.24.1.8-20.2022
Sutriadi MT, Harsanti ES, Wahyuni S, Wihardjaka A. 2020. Pestisida nabati: Prospek pengendali hama ramah lingkungan. Jurnal Sumberdaya Lahan. 13: 89. https://doi.org/10.21082/jsdl.v13n2.2019.89-101
Taj T, Sultana R, Shahin HD, Chakraborthy M, Ahmed GM. 2021. Phytol a phytoconstituent, its chemistry and pharmacological actions. Gis Science Journal [Internet]. [accessed on 2024 Jun 02]: 8: 395–406. Available on https://www.researchgate.net/publication/348351025
Wafaey AA, El-Hawary SS, Kirollos FN, Abdelhameed MF. 2023. An overview on Gliricidia sepium in the pharmaceutical aspect: A review article. Egyptian Journal of Chemistry. 66: 479–496. https://doi.org/10.21608/ejchem.2022.129184.5713
Wanjira EO, Muriuki J. 2019. Gliricidia sepium fact sheet: Establishment, management and benefits. World Agroforestry. 1–16.
Widiyaningrum P, Indriyanti DR, Ngabekti S, Abdillah ML, Labibah VR, Suwarti S. 2024. Analisis kemampuan dan kelangsungan hidup serangga Alphitobius diaperinus sebagai biodegradator sampah plastik. Bookchapter Konservasi Alam. 4: 22–23.
Wulandari DD, Santoso RAP. 2020. Pengaruh lama paparan pestisida terhadap aktivitas kolinesterase, serum glutamic oxaloacetic transaminase (SGOT) dan serum glutamic pyruvic transaminase (SGPT) pada pekerja yang terpapar pestisida golongan organofosfat. Jurnal Sains Dan Terapan Kimia. 14: 9. https://doi.org/10.20527/jstk.v14i1.6516
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