Detection of blaTEM Gene of Klebsiella pneumoniae Isolated from Swab of Food-Producing Animals in East Java

M. H. Effendi, I. G. Bintari, E. B. Aksono, I. P. Hermawan


Klebsiella pneumoniae is one of 9 bacteria resistance to antibiotics in concern. This research aimed to detect any gene of blaTEM in bacteria of the K. pneumoniae isolated from swab of food-producing animals. In this study, 195 swab samples were taken from 17 sampling locations. Samples obtained were cultivated on selective medium and had several tests including identification, antibiotic sensitivity test using Kirby-Bauer method against antibiotics of ampicillin, cefotaxime, amoxicillin, meropenem, and trimetrophrim-sulfamethoxazole, and followed by PCR test for detecting the gene that was responsible for the antibiotic resistances. The results showed that 10 out of 195 samples were found to be K. pneumonia, those were 4 samples originated from dairy cows (SP-S1, SP-S3, SP-B2, SP-G4), 2 samples originated from beef (SPT-K1, SPT-K2), 1 sample originated from chickens (A-W5), and 3 samples originated from fish (IN-P2, IN-P3, IN-S3). Most of  isolates (9/10) were found to be resistant toward amoxicillin. These isolates were SP-S3, SP-B2, SP-G4, SPT-KI, SPT-K2, A-W5, IN-P2, IN-P3, and the IN-S3 and all of them also showed to be positive of blaTEM gene. It could be concluded that most of K. pneumoniae isolates from food animals harbour had Extended Spectrum Beta-Lactamase (ESBL) encoding gene.


antibiotic resistance; ESBL; food-producing animals; Klebsiella pneumonie; amoxicillin

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Ahmed, A.M. & T. Shimammoto. 2011. Molecular characterization of antimicrobial resistance in gram-negative bacteria isolated from bovine mastitis in Egypt. Microbiol. Immunol. 55: 318-327.

Aljanaby, A.A.J & A.H.A. Alhasani. 2016. Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections. African Journal of Microbiology Research 10: 829-843.

Apriliani, N. P. E. U. & K. J. P. Pinatih. 2017. Prevalensi kelompok gen blactx-m-1 pada Klebsiella pneumoniae di Rumah Sakit Umum Pusat Sanglah Denpasar. E-Jurnal Medika 6: 1-7.

Brooks, G. F., J.S. Butel, & S.A. Morse. 2005. Medical Microbiology. Edisi I. Alih Bahasa: Bagian Mikrobiologi, FKU Unair. Salemba Medika. Jakarta.

Doosti, A., M. Pourabbas, A. Arshi, M. Chehelgerdi, & H. Kabiri. 2014. TEM and SHV genes in Klebsiella pneumoniae isolated from cockroaches and their antimicrobial resistance pattern. Osong Public Health and Research Perspectives 6: 3-8.

Ghasemi, Y., T.Archin, M.Kargar, & M.Mohkam. 2013. A Simple multiplex PCR for assecing prevalence of extended spectrum beta lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz Iran. Asian Pasific Journal of Tropical Medicine. 703-708.

Holt, S.R., E.G.D. Murray, & R.N. Smith. 2000. Bergey’s Manual Determinative of Bacteriology. 9th ed. Waverly Press, Baltimore.

Kusumaningrum, H. D., L. Handayani, & R. Novrianti. 2016. Partial sequencing of 16S rRNA gene of selected Staphylococcus aureus isolates and its antibiotic resistance. Med. Pet. 39: 67-74.

Lalzampuia, H., T. K. Dutta, I. Warjri & R. Chandra. 2014. Detection of extended-spectrum β-lactamases (blaCTX-M-1 and blaTEM) in Escherichia coli, Salmonella spp., and Klebsiella pneumoniae isolated from poultry in North Eastern India. Veterinary World 7: 1026-1031.

Lestari, N. W., A. Budiharjo, & A. Pangastuti. 2016. Bakteri Heterotrof aerobic asal saluran pencernaan ikan sidat (Anguilla bicolor bicolor) dan potensinya sebagai probiotik. Bioteknologi 13: 9-17.

Masruroh, C. A., M. B. Sudarwanto, & H. Latif. 2016. The Occurence of extended spectrum B-Lactamase-producing Escherichia coli from broiler feces in Bogor. JSV 34: 42-49

Monstein, H.J., A. Ostholm-Bulkhed, M.V. Nilsson, M. Dombusch, & L.E. Nilsson. 2007. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM, and blaCTX-M genes in Enterobacteriaceae. APMIS. 115:1400-1408.

Ningrum, S.G., R. D. Soejoedono, H. Latif, W. Arnafia, & I. W. T. Wibawan. 2016. Prevalence and characterization of shiga toxin-producing Escherichia coli isolated from slaughtered qurban animal in Jakarta Province. Med. Pet. 39: 90-94.

Overdevest, I., I. Willemsen, M. Rijnsburger, A. Eustace, X. Li, P. Hawkey, M. Heck, P. Savelkoul, C. Vandenbroucke-Grauls, K. van der Zwaluw, X. Huijsdens, & J. Klutmans. 2011. Extended-Spectrum β-laktamase genes of Escherichia coli in chicken meat and humans, the Netherlands. Emerg. Infect. Dis.17: 1216-1222.

Rahamathulla, M.P., B.N. Harish, L. Mataseje, & M.R. Mulvey. 2016. Carbapenem resistance mechanisms among blood isolates of Klebsiella pneumoniae and Escherichia coli. Journal. Microbiol. 10:45-53.

Sagita, D., L. Azizah, & Y. Sari. 2015. Identification of bacteria and antibiotic sensivity test of pus of post surgical wound infection in Jambi Public Hospital in the period August-October 2014. Sains Farmasi 1: 6-7.

Susilo, J., T. R. Sartono, & Sumarno. 2004. Deteksi bakteri Klebsiella pneumoniae pada sputum dengan metode imunohistokimia menggunakan anti outer membrane protein berat molekul 40 kDA Klebsiella pneumoniae sebagai antibodi. Jurnal Kedokteran Brawijaya 20: 12-18.

Wilopo, B.A.P., S. Sudigdoadi, E. Sahiratmadja, & I.M.W. Dewi. 2015. Loop-mediated isothermal amplification untuk mendeteksi gen blaTEM sebagai penyandi extended-spectrum beta-lactamase pada isolat enterobacteriaceae. Majalah Kedokteran Bandung 47: 243-244.

World Health Organization. 2014. Antimicrobial resistance: global report on surveillance.

Yeh, K, M., A. Kurup, L.K. Siu, Y.L.Koh, C.P. Fung, J.C. Lin, T.L. Chen, F.Y. Chang, & T.H. Koh. 2007. Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in singapore and Taiwan J. Clin. Microbiol. 45:466-471.

Yuwono, J. 2011. Prevalensi gen TEM pada extended spectrum beta lactamase producing enterobactericeae. Jurnal Kedokteran dan Kesehatan 43: 3098-3102.



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