Allelic Frequency of Kappa-Casein Locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole Grade (Bos indicus)

Authors

  • M A Mu'in
  • A Supriyantono

DOI:

https://doi.org/10.5398/medpet.2012.35.3.157

Keywords:

gene, locus, kappa-casein, PCR-RFLP, SIMPO

Abstract

This study was conducted to detect the genetic variants (single nucleotide polymorphism) of kappa-casein locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole grade (Bos indicus), SIMPO. Genomic DNA was isolated from blood sample of 40 SIMPO (21 males and 19 females). A 780 bp specific fragment of kappa-casein gene spanning from the forth exon region (517 bp) to forth intron (263 bp) was successfully amplified. The result of the PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphisms) analysis using HindIII enzyme showed that two genotypes (AA and AB) were found at this locus in SIMPO. The frequencies of A and B alleles in SIMPO were 0.79 and 0.21, respectively. The frequency lies between B. taurus (Simmental) and B. indicus group.

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Author Biographies

  • M A Mu'in

    Faculty of Animal Science, Fishery and Marine Science, Papua State University, Manokwari, West Papua

  • A Supriyantono

    Faculty of Animal Science, Fishery and Marine Science, Papua State University, Manokwari, West Papua

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Published

2013-02-21

Issue

Vol. 35 No. 3 (2012): Media Peternakan

Section

Articles

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