The Sensory Properties, Color, Microbial, Lipid Oxidation, and Residual Nitrite of Se’i Marinated with Lime and Roselle Calyces Extracts
AbstractMeat deterioration can occur because of lipid oxidation and bacteria that could affect meat quality. It has been recognized that fruits of lime (Citrus aurantifolia) and roselle (Hibiscus sabdariffa) calyces contain bioactive compounds that have a capability to prevent oxidation and bacterial growth. The objective of this research was to investigate the effect of lime and roselle calyces extracts on se’i (Rotenese smoked beef) quality. Completely randomized design (CRD) with 2x4 factorial pattern was used in this study. The first factor (E) was source of extracts i.e., lime extract (E1) and roselle extract (E2). The second factor (L) was level of the extract consisted of 4 levels i.e., control (without extract/ L0); L1= 1%; L2= 2%; and L3= 3% (v/v). Each treatment consisted of 3 replications. Sensory properties measured were aroma, taste, and tenderness. Other variables measured were color, total plate count (TPC), thiobarbituric acid reactive substances (TBARS), and residual nitrite. The taste and tenderness of se’i were affected (P<0.05) by combination of the extract and the level of the extract. Results showed that there were significant interactions (P<0.05) between the kind of extracts and the level of extract on L (lightness), a (redness), and b (yellowness) values, TPC, TBARS, and residual nitrite values. The level of 3% of lime extract as well as 3% of roselle calyces extract improved score of taste and tenderness, reduced a values, decreased TPC, TBARS, and residual nitrite values. Marinating in 3% of roselle calyces extract decreased the b value but marinating in 3% of lime increased the b value of se’i. It is concluded that marinating 3% of roselle or 3% of lime gives the best effect on taste, tenderness, TPC, and TBARS values of se’i.
AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Arlington, Virginia, USA.
Ardabili, A. G., R. Farhoosh, & M. H. H. Khodaparast. 2010. Frying stability of canola oil in presence of pumpkin seed and olive oils. Eur. J. Lipid Sci. Technol. 112: 871-877. https://doi.org/10.1002/ejlt.200900257
Bayram, M. & H. Bozkurt. 2007. The use of bulgur as a meat replacement: bulgur-sucuk (a vegetarian dry-fermented sausage). J. Sci. Food Agric. 87:411-419. https://doi.org/10.1002/jsfa.2712
Boshtam, M., J. Moshtaghian, & G. Naderi. 2011. Antioxidant effects of Citrus aurantifolia (Christm) juice and peel extract on LDL oxidation. J. Res. Med. Sci 16:951-955.
Bozkurt, H. & K. B. Belibagl. 2009. Use of Rosemary and Hibiscus sabdarifa L in production of Kavurma, a cooked meat product. J. Sci. Food Agric. 89: 1168. https://doi.org/10.1002/jsfa.3570
Dainty, R. H., R. A. Edwards, & C. M. Hibbard. 1985. Time course of volatile compounds formation during refrigerated storage of naturally contaminated beef in air. J. Appl. Bacteriol. 59:303-309. https://doi.org/10.1111/j.1365-2672.1985.tb03324.x
Demeyer, D., K. Honikel, & S. De Smet. 2008. The World Cancer Research Fund report 2007: a challenge for the meat processing industry. Meat Sci. 80:953–9. https://doi.org/10.1016/j.meatsci.2008.06.003
El-Alim, S. S. L., A. Lugasi, J. Hovari, & E. Dworschak. 1999. Culinary herbs inhibit lipid oxidation in raw and cooked minced meat patties during storage. J. Sci. Food Agric. 79:277-285. https://doi.org/10.1002/(SICI)1097-0010(199902)79:2<277::AID-JSFA181>3.0.CO;2-S
Ergonul, P.G. & C. Nergiz. 2010. Determination of organic acids in olive fruit by HPLC. Czech. J. Food. Sci. 28:202-205
Fernandez-Lopez, J., N. Zhi., L. Aleson-Carbonell., J. A. Perez-Alvarez, & V. Kuri. 2005. Antioxidant and antibacterial activities of natural extracts: application in beef meatballs. Meat Sci. 69: 371-380. https://doi.org/10.1016/j.meatsci.2004.08.004
Ghafar, M. F. A., K. N. Prasad., K. K. Weng, & A. Ismail. 2010. Flavonoid, hesperidine, total phenolic contents and antioxidant activities from citrus species. Afr. J. Biotechnol. 9: 326-330.
Harrigan, W. F. & M. E. McCance. 1976. Laboratory Methods in Food and Dairy Microbiology. Academic Press, London, U.K. p. 452.
Hussain, G., A. Rahman, T. Hussain, S. Uddin, & T. Ali. 2015. Citric and lactic acid effects on the growth Inhibition of E. coli and S. typhymurium on beef during storage. Sarhad Journal of Agriculture 31: 183-190. https://doi.org/10.17582/journal.sja/2015/126.96.36.199
Indonesian Health Department. 1999. Rule of Health Ministry of Indonesia. No. 1168/MENKES/PER/1999. Food additive.
Iqbal, A., O. E. Hany, A. Neelam, A. Alamgir, & S. Nawaz. 2016. In Vitro effects of papaya (Carica papaya), lemon juice (Lime), vinegar and yogurt as a meat tenderizer. J. Chem. Biol. Phys. Sci. 6(2): 568-573.
Karabacak. S. & H. Bozkurt. 2008. Effects of Urtica dioca and Hibiscus sabdarifa on the quality and safety of sucuk (Turkish dry-fermented sausage). Meat Sci. 78:288-296. https://doi.org/10.1016/j.meatsci.2007.06.013
Kilic, A. & A. Oztan. 2013. Effect of ascorbic acid utilization on cold smoked fish quality (oncorhynchus mykiss) during process and storage. Food Science and Technology Research 19:823-831. https://doi.org/10.3136/fstr.19.823
Kim, J. H., G. E. Hong, K. W. Lim, W. Park, & C.H. Lee. 2015. Influence of citric acid on the pink color and characteristics of sous vide processed chicken breasts during chill storage. Korean J. Food Sci. Anim. Resour. 2015. 35: 585–596. https://doi.org/10.5851/kosfa.2015.35.5.585
Klinhom, P., J. Klinhom, J. Senapa, & S. Methawiwat. 2015. Improving the quality of citric acid and calcium chloride marinated culled cow meat. Int. Food Res. J. 22:1410-1416.
Kowalski, J. & B. Wittrig. 2017. Simple, Reliable HPLC Analyses of Organic Acids Using Water–Compatible Allure or Ultra C18 Columns. Restek Corporation, U.S. 110 Benner Circle, Bellefonte.
Kumari, S., N. Sarmah, & A. K. Handique. 2013. Antioxidant activities of the unripen and ripen Citrus aurantifolia of assam. IJERSET 2:4811 - 4816
Mahadevan, N., Shivali, & P. Kamboj. 2009. Roselle Linn. An overview. Natural Product Radiance 8: 77-83.
Malelak, G. E. M., G. M. Sipahelut, I. G. N. Jelantik, M. R. Denoratu, & H. J. D. Lalel. 2015. Characteristics of se’i (rotenesse smoked meat) treated with coconut shell liquid smoked and lime extract. Med. Pet. 38: 89-94. https://doi.org/10.5398/medpet.2015.38.2.89
Marino, R., M. Albenzio, A. della Malva, A. Santillo, P.Loizzo, & A. Selvi. 2013. Proteolytic pattern of myofibrillar protein and meat tenderness as affected by breed and aging time. Meat Sci. 95:281-287. https://doi.org/10.1016/j.meatsci.2013.04.009
Mohd-Esa, N., F. S. Hern, A. Ismail, & C. L Yee. 2010. Antioxidant activity in different parts of roselle (Roselle L.) extracts and potential exploitation of the seeds. Food Chem. 122: 1055–1060. https://doi.org/10.1016/j.foodchem.2010.03.074
Mussa, S.B. & I.E. Sharaa. 2014. Analysis of vitamin C (ascorbic acid) contents packed fruit juice by UV-Visible Spectrophotometery and redox titration methods. IOSR Journal of Applied Physics. 6: 46-52. https://doi.org/10.9790/4861-06524652
National Agency for Drug and Food Control. 2013. Regulation of the Head of Indonesian National Agency for Drug and Food Control No. 36/2013 on the Maximum Limit of Preservative as Food Additive. Jakarta, Indonesia.
National Standardization Agency of Indonesia. 2009. Maximum permitted contaminated microbes in food. Indonesian National Standard (SNI)7388:2009.
Onibi, G.E. & I. B. Osho. 2007. Oxidative stability and bacteriological assessment of meat from broiler chickens fed diets containing Hibiscus sabdariffa calyces. African Journal of Biotechnology. 6: 2721-2726. https://doi.org/10.5897/AJB2007.000-2435
Patrascu, L., I. Dobre, & P. Alexe. 2013. Effect of thumbing time, injection rate and K-carrageenan addition on processing, textural and color characteristics of Biceps femoris muscle. Food Technol. 37: 69-84.
Sanchez del Pulgar, J., A. Gazques, & J. Ruiz-Carrascal. 2012. Physico-chemical, textural and structural characteristics of sous-vide cooked pork cheeks as affected by vacuum, cooking temperature and cooking time. Meat Sci. 90:828-835. https://doi.org/10.1016/j.meatsci.2011.11.024
Santos-Fandila, A., F. J. Camino-Sánchez, & A. Zafra-Gómez A 2014: Degradation markers in nutritional products: A review. Austin J. Anal. Pharm. Chem. 1: 1-7.
Sebranek, J. G., V. J. H. Sewalt, K. L. Robbins, & T. A. Houser. 2005. Comparison of a natural Rosemary extract and BHA/BHT for relative antioxidant effectiveness in pork sausage. Meat Sci. 69:289-296. https://doi.org/10.1016/j.meatsci.2004.07.010
Sen, A.R., B. M. Naveena, M. Muthukumar, & S. Vaithiyanathan. 2014. Colour, myoglobin denaturation and storage stability of raw and cooked mutton chops at different end point cooking temperature. J. Food Sci. Technol. 51: 970–975. https://doi.org/10.1007/s13197-011-0557-z
Shah, M. A., S. J. D. Bosco, & S. A. Mir. 2014: Plant extracts as natural antioxidants in meat and meat products. Meat Sci. 98: 21-33. https://doi.org/10.1016/j.meatsci.2014.03.020
Viuda-Martos, M., J. Fernández-López, E. Sayas-Barbera, E. Sendra, C. Navarro, & J. A. Perez- Alvarez. 2009. Citrus co-products as technological strategy to reduce residual nitrate content in meat products. J. Food Sci. 74:93-100. https://doi.org/10.1111/j.1750-3841.2009.01334.x
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