Vol 38, No 3 (2010)

Jurnal Agronomi Indonesia

Table of Contents

Articles

Anther culture is one of tissue culture methods which can be applied to plant breeding programs in order to accelerate the process of obtaining pure lines. The successful development of rice varieties is highly dependent on genetic diversity and desirable traits. To obtain the genetic variability of doubled haploid lines through anther culture techniques, anther F1 or F2 were used as explants sources. The objectives of the study were to select and characterize doubled haploid lines of upland rice having the characters of new plant type, and to study the genetic variability and agronomic characters of tested doubled haploid lines. A total of 58 doubled haploid lines, and four parents i.e. Fatmawati, SGJT-28, SGJT-36, and Way Rarem were used in this study. The experiment used completely randomized design with three replications. Results showed that the characters of the doubled haploid lines vary considerably. Selection of the character i.e. number of productive tillers, number of filled grain per panicle, and percentage of empty grain was more effective to be selected because they were well correlated to weight of grain per hill, possessed high heritability values, and have wide genetic variability. Based on productive tillers number, number of filled grains per panicle, fertility, weight of 1,000 grains, and weight of grains per hill, the lines of P3-26, P3-27, P3-28, P4-45, P5-50, P6-103, P6-105,  P3-120, P3-134, P3-135, P3-150, P3-158, P3-248, P3-249, P6-271, P6-272, P6-274, P6-276, and  P6-295 were potential for further selection for new type of upland rice.

 

Keywords:  variability, characters, doubled haploid, new plant type, upland rice

Reny Herawati, Bambang S. Purwoko, Iswari S. Dewi
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, Susilawati, Bambang S. Purwoko, Hajria l Aswidinnoor, Edi Santosa
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Bacterial leaf blight, the disease caused by Xanthomonas oryzae pv. oryzae (Xoo), a seedborne pathogen, has been known to reduce rice yield by 50%. In this study, two consecutive experiments were conducted in order to investigate the effect of various biological seed treatments applied on rice seeds artificially infected by Xoo on seed quality and seedling growth. Laboratory experiment was arranged in a completely randomized design while greenhouse experiment was arranged in a completely randomized block design. In both experiments, 12 seed treatments were applied: un-infected seeds (negative control) without seed treatment, Xoo infected seeds (positive control) without seed treatment, infected seeds soaked in bactericide Agrept  0.2%, infected seeds soaked in biological agent suspension isolate A6, infected seeds soaked in A54 isolate, infected seeds soaked in 5/B isolate, infected seeds soaked in 11/C isolate, infected seeds matriconditioned + A6 isolate, infected seeds matriconditioned + A54 isolate, infected seeds matriconditioned + 5/B isolate, and infected seeds matriconditioned + 11/C isolate. Soaking seeds either in Agrept  0.2% or in biological agent suspension was conducted for 30 h. Matriconditioning was conducted using ratio of seeds to carrier (burned rice hull 32 mesh) to biological agent suspension or bactericide solution of 1.0 (g) :0.8 (g): 1.2 (ml) for 30 h in air-conditioned room ca. 25 oC. Results of experiments showed that all biological seed treatments could suppress Xoo in rice seeds. Matriconditioning plus biological agent (isolate A6), biopriming with isolate A6 or isolate A54 were the best seed treatments to improve seed viability and vigor. In greenhouse experiment, matriconditioning plus isolate A54 was the best seed treatment to increase seedling growth.

 

Keywords: biopriming, matriconditioning, rhizobacteria, seed health, viability, vigor
, Agustiansyah, Satriyas Ilyas, , Sudarsono, Muhammad Machmud
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Husni Thamrin Sebayang, Agus Suryanto, Tristi Indah Dwi Kurnia
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Aromatic new plant type (NPT) rice lines were selected to obtain high yielding and aromatic lines. The objectives of the research were to study the yield stability and adaptability of 35 NPT rice lines across different environment, with Ciherang and Sintanur as check varieties. The lines planted at two locations, Bogor and Pusakanagara in two seasons (2009 dry and wet seasons, DS-WS). The experiment used  randomized complete block design (RCBD), with three replications. The 21-day-old seedlings were planted with spacing of 20 cm x 20 cm, with a plot size of 2 m x 5 m. Yield stability and adaptability were estimated by using coefficient regression (bi) and general mean of yield by Finlay-Wilkinson method. Combined analysis of variance showed that lines (G), environment (E), and the G x E interaction were significantly different. Lines showed different stability and adaptability. Several lines yielded higher than Ciherang variety. Nine lines were classified as stable and widely adapted at the marginal environment, i.e IPB 116-F-3-1, IPB 117-F-4-1, IPB-117-F-14-2, IPB-117-F-15-2, IPB-117-F-17-5, IPB 140-F-1-1, 140-F-IPB, 2-1, IPB 140-F-3, and IPB 149-F-2. Lines IPB 113-F-2, IPB 140-F-4, IPB 140-F-6, IPB 140-F-7, and B11738-MR-Si-1-2-1-2 were not stable and adapted only in optimum environmental condition (bi > 1) while IPB 116-F-46-1, IPB-117-F 17-4, IPB-117-F 18-3 and B11955-MR-84-1-4 has the value of bi < 1 or adaptable to marginal environments.

 

Keywords:  NPT rice, aromatic rice, yield stability
Angelita Puji Lestari, Buang Abdullah, Ahmad Junaedi, Hajrial Aswidinnoor
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Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including  plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV  infection. Shoot-tip explants of five chilli pepper genotypes (Jatilaba, ICPN 12 no. 4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5%  1.0% and control),  and incubation time (15, 30, 60 min). Subsequently, each explant was grown  in multiplication media (MS media + 5 mg L-1 BAP + 0.5 mg L-1 TDZ), rooting media (MS  media +  1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost  2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate  20.4 %  was achieved with 0.5% EMS in combination with 60 min  incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2,  survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confirmed that  4.09% of the somaclonal progenies were  resistance to ChiVMV.

 

Keywords:  Capsicum annuum, ChiVMV, ethyl methane sulfonate, induce mutation, resistance
Ifa Manzila, Sri Hendrastuti Hidayat, Ika Mariska, Sriani Sujiprihati
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Trias Sitaresmi, Sriani Sujiprihati, Muhamad Syukur
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Widi Agustin, Satriyas Ilyas, Sri Wilarso Budi, Iswandi Anas, Faiza C. Suwarno
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Yard long bean (Vigna unguilata L.) 777 was grown in Ultisols, which typically have low pH and high P-fixation, to determine the best correlation of soil extraction methods for soil P with yields, and to develop soil P response categories.  The research was conducted at SANREM base camp in Hambaro Village, Nanggung, Bogor, Indonesia from April-August 2008. Treatments were arranged in a Split Plot Design with three replications. The main plots were treatments with soil P status of 0X, ¼X, ½X, ¾X and X, where is X = 1,590.5 kg SP-36 ha-1 (36% P2O5) applied once  a month before planting.  The subplots were P application rate of   0, 75, 150, 225 and 300 kg P2O5 ha-1. Yard long beans were planted in double rows per bed, 60 cm between rows and 25 cm within rows, 2 seeds per hole, with plot size of  1.5 m x 5 m. Coefficient correlation (r) of  extraction reagents Olsen, Bray-1, HCl 25%, and Mechlich-1 were 0.772, 0.765, 0.755, and 0.732, respectively.  Based on Olsen soil testing methods, soil response categories of very low, low, medium, and high were (ppm P2O5) ≤ 18.40, 18.40 < P < 117.27, 117.27 < P < 267.04, and ≥ 267.04 extracted-P, respectively. Based on Bray-1 soil testing methods, soil response categories for low, medium, and high were ≤ 87.81, 87.81

2O5), respectively. Fertilizer recommendation based on the Olsen soil test for low response category was 185.75 kg P2O5 ha-1, and for the medium soil category was 175.97 kg P2O5 ha-1.  The Bray-1 soil test for the low response category was 184.31 kg P2O5 ha-1, and for the medium soil category was 161.39 kg P2O5 ha-1.

 

Keywords: fertilizer recommendation, calibration, phosphorus, yard long bean
Anas Dinurrohman Susila, Juang Gema Kartika, Tisna Prasetyo, Manuel Celiz Palada
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Ani Kurniawati, Roedhy Poerwanto, , Sobir, Darda Effendi, Herry Cahyana
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Yoko Mine, Edi Santosa, Wakanori Amaki, Nobuo Sugiyama
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M. Zulman Harja Utama
M. Zulman Harja Utama
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