Screening and Identification of Plant Growth Promotion Endophyte from Five Sugarcane Varieties in PT Gunung Madu Plantations Lampung

Seleksi dan Identifikasi Endofit Pemacu Pertumbuhan dari Lima Varietas Tebu di PT Gunung Madu Plantations Lampung

  • Remaja Sitepu Program Studi Bioteknologi Tanah dan Lingkungan, Sekolah Pascasarjana, Institut Pertanian Bogor, Jl. Meranti Kampus IPB Dramaga, Bogor 16680
  • Suryo Wiyono Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor, Jl. Meranti Kampus IPB Darmaga Bogor 16680
  • Dwi Andreas Santosa Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, Institut Pertanian Bogor, Jl. Meranti Kampus IPB Darmaga Bogor 16680
Keywords: Bacillus safensis, Bacteria, Hypersensitive reaction test

Abstract

Endophyte is the bacteria or fungi which are associate and colonize of internal plant tissues naturally without negatively impacting of their host. A number of endophytes are useful to plants because play a role as plant growth promotion or as biological control agent. The purpose of this study were to select and identify of plant growth promotion endophytes from five sugarcane varieties in PT Gunung Madu Plantations Lampung. A total of 82 isolates that had passed from hypersensitive reaction and hemolysis test were screened in two stages. The first stage was performed in laboratory by inoculating of the endophytes on the rice seed and evaluating of the sprouts performance after seven days. Isolates that passed in the first stage were continued to the second by inoculating them to the sugarcane seed in semi-field test. Growth of the sugarcane plant test was evaluated up to six weeks after planting. A total of 9 bacteria and 9 fungi isolates were indicated as plant growth promotion in the first stage, but only two bacterial and one fungal isolates were consistent in the second stage. Molecular identification and alignment of the sequences on the BLAST showed that N12 (isolated from GMP3 root) was 100% identical with Bacillus safensis, L16 (isolated from GMP3 leave) was 99% identical with Domibacillus robiginosus, and C78 (isolated from PS48 stem) was 99% identical with Cladosporium cladosporioides. The three isolates were able to produce of auxin, cytokines, and gibberellins, but B. safensis is higher than the other two.

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References

Balint, M., P. Tiffin, B. Hallstrom, R.B. O’Hara, M.S. Olson, J.D. Fankhauser, M. Piepenbring and I. Schmitt. 2013. Host genotype shapes the foliar fungal microbiome of balsam poplar (Populus balsamifera). Plos one, 8:1-9.

Bensch, K., J.Z. Groenewald, J. Dijksterhuis, M. Starink-Willemse, B. Andersen, B.A. Summerell, H.D. Shin, F.M. Dugan, H.J. Schroers, U. Braun and P.W. Crous. 2010. Species and ecological diversity within the Cladosporium cladosporioides complex (Davidiellaceae, Capnodiales). Studies in Mycology, 67:1-94. Doi:10.3114/sim.2010.67.01.

Bertalan, M., R. Albano, V. de Pádua, L: Rouws, C. Rojas, A. Hemerly, K. Teixeira, S. Schwab, J. Araujo and Oliveira. 2009. Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5. BMC Genomics, 10:1-17.

Favaro, L.C.L., F.L.S. Sebastianes and W.L. Araujo. 2012. Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth. Plos One, 7:1-10.

Gomez, K.A. and A.A. Gomez. 1984. Statistical procedures for agricultural research (2nd Ed.). John wiley and sons, New York. 680 pp.

[GMP] PT Gunung Madu Plantations. 2011. Tim peneliti GMP: Usulan pelepasan varietas tebu unggul harapan RGM99-515 dan RGM99-599 spesifik lokasi lahan kering ultisol beriklim tropis basah. PT Gunung Madu Plantations, Lampung. 17 pp.

[GMP] PT Gunung Madu Plantations. 2012. Buku pedoman teknis budidaya tebu di GMP tahun 2012/2013. PT Gunung Madu Plantations, Lampung. 43 pp.

Hallmann, J., A. Quadt-Hallmann, W.F. Mahaffee and J.W. Kloepper. 1997. Bacterial endophytes in agricultural crops. Can. J. Microbial, 43:895-914.

Hamayun, M., S.A. Khan, A.L. Khan, G. Rehman, Y.H. Kim, I. Iqbal, J. Hussain, E.Y. Sohn and I.J. Lee. 2010. Gibberellin production and plant growth promotion from pure cultures of Cladosporium sp. MH-6 isolated from cucumber (Cucumis sativus L.). Mycologia, 102:989-95. Doi: 10.3852/09-261.

Hidayati, U. 2014. Potensi bakteri endofit asal tanaman karet sebagai pemacu pertumbuhan bibit batang bawah tanaman karet (Hevea brasiliensis müll. Arg.) [Disertasi]. IPB. Bogor.

Hudson, A.O., N.H. Ahmad, R. van Buren and M.A. Savka. 2010. Sugarcane and grapevine endophytic bacteria: Isolation, detection of quorum sensing signals and identification by 16S v3 rDNA sequence analysis In A. Mendez-Vilas (Eds.). Current Research, Technology and Education Topic in Applied Microbiology and Microbial Biotechnology. Formatex: 801-806.

[ICBB] Indonesian Center for Biodiversity and Biotechnology. 2016. Laporan hasil uji analisa No: ICBB.LHP.XII.2016.0996–0999. Bogor.

Lateef, A., I.A. Adelere and E.B.G. Kana. 2015. The biology and potential biotechnological applications of Bacillus safensis. Biologia, 70:411-419. Doi: 10.1515/biolog-2015-0062.

Loiret, F.G., E. Ortega, D. Kleiner, P. Ortega-Rodes, R. Rodes and Z. Dong. 2004. A putative new endophytic nitrogen-fixing bacterium Pantoea sp. from sugarcane. J. Appl. Microb., 97: 504-511.

Mandic-Mulec, I., P. Stefanic and J.D. van Elsas. 2015. Ecology of Bacillaceae. Microbiol Spectrum 3:TBS-0017-2013. Doi: 10.1128/microbiolspec.TBS-0017-2013.

Matsuoka, S., and R. Stolf. 2012. Sugarcane tillering and ratooning: key factor for profitable cropping. In J.F. Goncalves and K.D. Correia (Eds.). Sugarcane: Production, Cultivation and Uses. Nova Sci. Publ. p. 137-157.

Mendes, R., A.A. Pizzirani-Kleiner, W.L. Araujo and J.M. Raaijmakers. 2007. Diversity of cultivated endophytic bacteria from sugarcane: Genetic and biochemical characterization of Burkholderia cepacia complex isolates. Appl. Environ. Microb., 73:7259-7267.

Ming, R., P.H. Moore, K.K. Wu, A. D'Hont, J.C. Glaszmann and T.L. Tew. 2006. Sugarcane improvement through breeding and biotechnology. Plant Breeding Reviews, 27:15-118.

Njoloma, J.P., M. Oota, K. Taroura, Y. Saeki, and S. Akao. 2006. Colonization ability of Herbaspirillum spp. B501gfp1 in sugarcane, a non-host plant in the presence of indigenous diazotrophic endophytes. Afr. J. Biotech., 5:836-841.

Paul, N.C., and S.H. Yu. 2008. Species of endophytic Cladosporium in pine trees in Korea. Mycobiology, 36:211-216.

Seiler, H., M. Wenning, and S. Scherer. 2013. Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room. Int. J. Syst. Evol. Microbiol., 63:2054-61. Doi: 10.1099/ij s.0.044396-0.

Published
2018-04-01
How to Cite
Sitepu, R., Wiyono, S., & Santosa, D. A. (2018). Screening and Identification of Plant Growth Promotion Endophyte from Five Sugarcane Varieties in PT Gunung Madu Plantations Lampung: Seleksi dan Identifikasi Endofit Pemacu Pertumbuhan dari Lima Varietas Tebu di PT Gunung Madu Plantations Lampung. Jurnal Ilmu Tanah Dan Lingkungan, 20(1), 19-24. https://doi.org/10.29244/jitl.20.1.19-24