Jurnal Fitopatologi Indonesia http://journal.ipb.ac.id/index.php/jfiti <p><img src="/public/site/images/jfiti/Header_OJS-JFI_copy.jpg" width="689" height="86"></p> <p>&nbsp;</p> <p><strong>Jurnal Fitopatologi Indonesia (JFI)</strong>&nbsp;is&nbsp;an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).</p> <p><strong>JFI&nbsp;</strong>is an open-access, peer reviewed journal that has been&nbsp;<strong>Accredited by Directorate General of Higher Education (DGHE), Republic of Indonesia</strong><strong>&nbsp;No. 12/M/Kp/II/2015&nbsp;</strong>which is valid for 5 (five) years since enacted on 11&nbsp;February 2015.&nbsp;<strong>JFI</strong>&nbsp;includes manuscript articles relating to plant diseases and their control, including pathogenic characterization, pathogen detection and identification, physiology and biochemistry of diseases, molecular biology, morphology and ultrastructure, genetics, disease transmission, ecology and epidemiology, chemical and biological control, Topics about abiotic abnormalities in plant health.&nbsp;<strong>Articles can be either original research papers, short communications, or disease note.</strong>&nbsp;<br><br><a href="http://u.lipi.go.id/1180435451">P-ISSN: 0215-7950</a><br><a href="http://u.lipi.go.id/1383537868">E-ISSN: 2339-2479</a></p> <p>Download:&nbsp;<a href="http://journal.ipb.ac.id/index.php/jfiti/manager/files/PedomanJuli2016.pdf">Author Guideline</a></p> The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia) en-US Jurnal Fitopatologi Indonesia 0215-7950 <p>Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:</p> <ol> <li class="show">&nbsp;Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> <li class="show">&nbsp;Authors submitting manuscripts should understand and agree that copyright of manuscripts published are held by Jurnal Fitopatologi Indonesia. Copyright encompass exclusive rights to reproduce, to distribute, and to sell any part of the journal articles in all form and media. &nbsp;The reproduction of any part of this journal, its storage in databases and its transmission by any form or media will be allowed only with a written permission from Jurnal Fitopatologi Indonesia.</li> <li class="show">&nbsp;Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work, with an acknowledgement of its initial publication in this journal.</li> <li class="show">&nbsp;Authors are permitted and encouraged to post their work online prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.</li> </ol> Deteksi dan Identifikasi Nematoda Puru Akar (Meloidogyne spp.) pada Tanaman Bit Menggunakan Metode DNA Barcoding http://journal.ipb.ac.id/index.php/jfiti/article/view/26384 <p><strong>Detection and Identification Root Knot Nematode (<em>Meloidogyne</em> spp.) in Sugar Beet Using DNA Barcoding Method</strong></p> <p>Nematode identification based on morphological characters requires high accuracy and needs to be supported by molecular identification results. One method of molecular identification is DNA barcodes. DNA barcode is a molecular identification using the cythochrome oxidase sub unit 1 (CO1) gene. This study aims to identify NPA associated with beetroot based on PCR and <em>Meloidogyne</em> CO1 nucleotide gene sequencing. Beetroot samples were obtained from agricultural land located in Cipanas District, Cianjur Regency, West Java. Sampling was done by purposive sampling method. Morphological identification by observing the perineal pattern of female NPA. Molecular identification includes extraction, amplification, DNA visualization, nucleotide and phylogenetic analysis. Morphological perineal pattern observations obtained <em>M. incognita</em> and <em>M. arenaria</em> species. DNA amplification was carried out using CO1SIF (5’-GCCTGCATTTGGTTAG-‘3) and CO1SIR (5’-TCAAACCAGTCCT-‘3), CO1SAF (5’-GGGTACTGGATGAACATTA-‘3) and CO1SAR (5’-ACTTCAGGATGACCAAA-‘3). DNA amplification succeeded in getting a DNA band measuring ± 360 for <em>M. arenaria</em> and ± 326 for <em>M. incognita</em>. The sequencing results showed that <em>M. incognita</em> isolates from Indonesia were closely related to <em>M. incognita</em> isolates from China, US, UK, and Vietnam with a 100% homology level. The results of the phylogeny analysis showed that <em>M. incognita </em>Indonesia was still in the same group as <em>M. incognita</em> from China, US, UK, and Vietnam. <em>M. arenaria</em> isolates were closely related to <em>M. arenaria </em>isolates from Argentina and US with a homology level of 100%. The results of phylogenetic analysis showed that <em>M. arenaria</em> from Indonesia was still in the same group as <em>M. arenaria</em> from Argentina and US.</p> Ni Wayan Kartika Pratiwi Rosyid Amrulloh Fawwaz El Auly Fitrianingrum Kurniawati Copyright (c) 2020 Jurnal Fitopatologi Indonesia 2020-01-31 2020-01-31 16 1 1 8 10.14692/jfi.16.1.1-8 Insidensi Penyakit, Identifikasi dan Keragaman Bakteri Burkholderia glumae, Penyebab Penyakit Busuk Bulir pada Beberapa Varietas Padi di Jawa Barat http://journal.ipb.ac.id/index.php/jfiti/article/view/26350 <p><strong>Incidence of Bacterial Grain Rot Disease, Identification, and Diversity of <em>Burkolderia glumae</em> in Some Rice Varieties in West Java</strong></p> <p>Rice rot disease caused by the bacteria <em>Burkholderia glumae</em> needs to be looked out, including in West Java as one of rice production center for Indonesia. This study was carried out to determine disease incidence, identity and diversity of <em>B. glumae</em> in several rice varieties grown in West Java Province. Sampling and observation of disease incidence were conducted in 9 districts.&nbsp; Bacteria were isolated from rice grains with symptomatic rot, followed by biochemical and physiological tests involving Gram and growing tests at pH 4.8 and 2% NaCl.&nbsp; Phenotypic variation was observed from the colony color on S-PG media, toxofalvin production, hypersensitivity response to tobacco leaves, and pathogenicity test on rice plants.&nbsp; <em>B. glumae</em> specific primers, JLBgF/JLBgR, and bacteria universal 16S rRNA primers, 27F/1492R were used to determine molecular identity. Genotypic diversity analysis was performed using neighbour-joining tree method. Disease incidence was in the range of 0–73.3%, the highest was found in Dawuan District (Karawang) on var. Mekongga. The phenotypic observations showed 10 isolates belonging to type A colonies and 19 isolates of type B; 25 isolates produced toxoflavin; 29 isolates produced hypersensitivity responses to tobacco leaves and blight symptoms in rice plants. Identification using specific and universal primers confirmed that 29 isolates were <em>B. glumae</em>. The genotypic diversity analysis of 16S rRNA gene showed that KRCH-2 isolates (Karawang) and INCH-6 (Indramayu) were closely related to <em>B. glumae</em> from China and America.</p> Ani Widarti Giyanto Giyanto Kikin Hamzah Mutaqin Copyright (c) 2020 Jurnal Fitopatologi Indonesia 2020-01-31 2020-01-31 16 1 9 20 10.14692/jfi.16.1.9-20 Laju fotosintesis pada tanaman kelapa sawit terinfeksi karat daun Cephaleuros virescen http://journal.ipb.ac.id/index.php/jfiti/article/view/27094 <p><strong>Photosynthesis Rate of Oil Palm Infected by Red Rust Cephaleuros virescens</strong></p> <p>The incidence of oil palm red rust disease caused by <em>Cephaleuros virescens </em>tends to increase in Indonesia. However, the loses due to this disease has never been measured quantitatively. This study was conducted to measure the rate of photosynthesis on 5-year-old palms with 3 infection categories, i.e. mild, medium, and heavy infection. Field observation of oil palm in Kalianta showed that the infection of red rust disease occurred starting from lower to the upper fronds. Higher infection was prevalently on the adaxial than abaxial leaf. The red rust infection on frond number 17 or younger severely affects the photoshynthesis rate of the oil palm. On the palm with heavy infection category, the photosynthesis rate on frond number 9 was decreased up to 42.48%, i.e. from 15.51 µmol to 8.92 µmol, compared to that with mild infection.</p> Agus Susanto Agus Eko Prasetyo Hari Priwiratama Muhdan Syarovi Copyright (c) 2020 Jurnal Fitopatologi Indonesia 2020-01-31 2020-01-31 16 1 21 29 10.14692/jfi.16.1.21-29 Bakteri Agens Hayati Potensial terhadap Patogen Penting pada Padi http://journal.ipb.ac.id/index.php/jfiti/article/view/27848 <p><strong>The Potential Biological Agent Bacteria Against for Controling Important Pathogens on Rice</strong></p> <p>At present, biological control technology for the main diseases of rice continues to grow. In the development of biological control technology, inhibition mechanism of pathogens in the development of disease in a plant population in a certain area becomes important. The aim of this study was to obtain potential biological agent bacteria for controlling important rice diseases based on antagonistic mechanism, ability to induce plant resistance and support plant fitness, and their compatibility. The results showed that <em>Ralstonia pickettii</em> TT47, <em>Pseudomonas fluorescens</em> P12, <em>Chromobacterium</em> sp. T51118, <em>Bacillus subtilis</em> 451 and 154, and <em>Streptomyces</em> sp. T51105 have an antibiosis mechanism by producing secondary metabolites and volatile compounds. Additionaly, <em>Chromobacterium</em> sp. and <em>Streptomyces</em> sp. also have a lysis mechanism on the basis of the chitinolityc enzyme production test. The antibiotic activity of <em>R. pickettii</em> and <em>P. fluorescens</em> were strong to <em>P. oryzae</em> on dual culture test with the highest inhibition up to 79.68% and 77.59% respectively. Inhibition growth of <em>P. oryzae</em> and <em>R. solani</em> mycelium on volatile tests up to 100% by <em>Chromobacterium</em> sp. T51118. Generally, all of biological agents were able to induce plant resistance and support to plant fitness. Compatibility test obtained <em>R. pickettii</em>, <em>P. fluorescens</em>, and <em>Chromobacterium</em> sp. were compatible. Based on the results, three biocontrol agent bacteria, namely <em>P. fluorescens</em> P12, <em>R. pickettii</em> TT47, and <em>Chromobacterium</em> sp. T51118 were excellent. They were able to suppress the growth of pathogens, were able to induce plant resistance and support plant fitness, as well as they have more diverse target pathogens, and compatible.</p> Ratna Sari Dewi Giyanto Giyanto Meity Suradji Sinaga Dadang Dadang Bambang Nuryanto Copyright (c) 2020 Jurnal Fitopatologi Indonesia 2020-01-31 2020-01-31 16 1 37 48 10.14692/jfi.16.1.37-48 Penyakit Mosaik Kuning Pada Kedelai http://journal.ipb.ac.id/index.php/jfiti/article/view/26786 <p><strong>Yellow mosaic Disease on Soybean</strong></p> <p>&nbsp;</p> <p>Epidemic of yellow mosaic disease had occurred in yard long bean (<em>Vigna unguiculata </em>subsp. <em>sesquipedalis</em>) in Java in 2008. However, this disease has never been reported in other areas in Indonesia. Observations on soybean plants in Cirebon (West Java), Bantul (DI Yogyakarta), and Musi Banyuasin (South Sumatra) showed systemic symptoms of yellow mosaic on leaves with vein banding. Plant samples showing yellow mosaic symptoms were taken for virus detection using PCR (polymerase chain reaction). The amplification results with specific MYMIV primers showed 1000 bp DNA bands. The results of analysis nucleotide sequence homology showed MYMIV Cirebon has a high homology (98.6-99.2 %) with MYMIV from Tegal, Subang, Klaten, Magelang, Brebes, and Bogor. This confirmed that the yellow mosaic symptom leaf samples from Bantul, Musi Banyuasin, and Cirebon were positively infected with MYMIV. Phylogenetic analysis shows that the Cirebon MYMIV isolates formed a group with all MYMIV sequences in Indonesia and Oman, and separated from MYMIV groups from other countries namely Bangladesh, Nepal, Pakistan and India. MYMIV Cirebon isolate was registered in GenBank with accession number LC481009. MYMIV has been reported in soybean in Java (2013). This finding is the first report of MYMIV infection in soybeans in South Sumatera.</p> Mimi Sutrawati Sri Hendrastuti Hidayat Bonny Purnomo Wahyu Soekarno Ali Nurmansyah Copyright (c) 2020 Jurnal Fitopatologi Indonesia 2020-01-31 2020-01-31 16 1 30 36 10.14692/jfi.16.1.30-36