Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara

  • Budi Sri Utami Institut Pertanian Bogor
  • Supramana Supramana Institut Pertanian Bogor
  • Giyanto Giyanto Institut Pertanian Bogor
DOI: https://doi.org/10.14692/jfi.13.3.98

Abstract

Root-knot nematode (Meloidogyne spp.). is one of the main constraint of potato production in North Sulawesi.  Little is known about Meloidogyne species infecting potatoes in North Sulawesi.  Therefore, research was conducted to identify Meloidogyne spp. on potatoes in North Sulawesi and further study their relationship with related species from other countries.  Infected potato tubers with pimple-like knot symptom were collected from three potato production centers, i.e. Kakenturan (South Minahasa), Purworejo (East Bolaang Mongondow) and Singsingon (…?? Bolaang Mongondow).  Morphological identification was conducted based on the perineal pattern of  the female; whereas molecular identification was conducted by PCR using specific primer for ITS-rDNA, followed by DNA sequencing and phylogenetic analysis. Two Meloidogyne species were identified i.e.  M. javanica (samples form Kakenturan, Purworejo and Singsingon) and M. incognita (samples from Purworejo). M. javanica and M. incognita from North Sulawesi are similar to the related species from China with homology level of 97.5 % and 100 %, respectively.

Downloads

Download data is not yet available.
Published
2017-09-06
How to Cite
UtamiB. S., SupramanaS., & GiyantoG. (2017). Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara. Jurnal Fitopatologi Indonesia, 13(3), 98. https://doi.org/10.14692/jfi.13.3.98
Issue
Vol. 13 No. 3 (2017)
Section
Articles

Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:

1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.

3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.