Main Article Content
Root knot nematodes is an important pathogen on potatoes in tropical and sub-tropical areas. Root knot nematodes contribute a significant impact in reducing the quality and quantity of potato tuber. Meloidogyne incognita is one of the species causing the root knot. This research was conducted to identify M. incognita on potatoes in Java Island based on morphological and DNA molecular characteristic. The infected potato tubers with pimple-like knot symptom were collected from Pangalengan (West Java), Banjarnegara (Central Java), and Kota Batu (East Java). Nematode was identified based on morphological character of perineal pattern, and based on molecular DNA character by polymerase chain reaction technique using a pair of specific primer (MI-F and MI-R), followed by DNA fragment sequencing and phylogenetic analysis. Based on morphological character of perineal pattern, M. incognita was detected in all 3 locations; while based on DNA molecular character, and M. incognita was detected in Pangalengan (West Java) and Kota Batu (East Java). M. incognita from Pangalengan had high homology, i.e.99.2% to 99.8% with those isolates from China, India, and Malaysia.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.