View of <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;text-indent:14.15pt;line-height:normal;"><strong><span style="font-size:9pt;">Soybean is a good source of protein. It has two major fractions, </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin (7S) and glycinin (11S). </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous</span></strong><strong><span style="font-size:5pt;"> </span></strong><strong><span style="font-size:9pt;">cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin and </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin sub unit-</span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;"> level obtained from two varieties of soybean i.e. <em>Wilis</em> and <em>Detam 1</em> varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that <em>Detam</em> variety and raw seed contained the highest total </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin level. And Panthee method was the best method for extraction of total </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin, while Deak method was the best method for extraction of </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin subunit-</span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">.</span></strong></p>

Return to Article Details <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;text-indent:14.15pt;line-height:normal;"><strong><span style="font-size:9pt;">Soybean is a good source of protein. It has two major fractions, </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin (7S) and glycinin (11S). </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous</span></strong><strong><span style="font-size:5pt;"> </span></strong><strong><span style="font-size:9pt;">cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin and </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin sub unit-</span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;"> level obtained from two varieties of soybean i.e. <em>Wilis</em> and <em>Detam 1</em> varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that <em>Detam</em> variety and raw seed contained the highest total </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin level. And Panthee method was the best method for extraction of total </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin, while Deak method was the best method for extraction of </span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">-conglycinin subunit-</span></strong><strong><span style="font-size:9pt;font-family:Symbol;">b</span></strong><strong><span style="font-size:9pt;">.</span></strong></p> Download Download PDF