<p class="MsoNormal" style="text-align:justify;text-indent:14.15pt;"><strong><span style="font-size:9pt;">Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (<em>Creb-2</em>) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (<em>Creb-2</em>) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F</span></strong><strong><span style="font-size:5pt;">2</span></strong><strong><span style="font-size:9pt;"><span> </span>plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to <em>Creb-2</em> in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect <em>Creb-2</em> in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.</span></strong></p> <p class="MsoBodyText2" style="line-height:10.8pt;"><strong><span style="font-size:9pt;"> </span></strong></p> <p class="MsoBodyText2" style="margin-left:58.1pt;text-indent:-58.1pt;line-height:10.8pt;"><strong><span style="font-size:9pt;"><span> </span></span></strong><span style="font-size:9pt;">Key words: <em>Cucumis melo</em> L., <em>Creb-2</em>, RAPD, SCAR<strong></strong></span></p>

Abstract

Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.

      Key words: Cucumis melo L., Creb-2, RAPD, SCAR