Isolation and Characterization of C-C Chemokine Ligand 7 (CCL7) in Cynomolgus Macaques

  • Sela S. Mariya Primate Research Center, Bogor Agricultural University, Bogor
  • Fitriya N. Dewi Primate Research Center, Bogor Agricultural University, Bogor Bimana Indomedical, Bogor
  • Villiandra Villiandra Bimana Indomedical, Bogor
  • Yasmina A. Paramastri Primate Research Center, Bogor Agricultural University, Bogor
  • Diah Iskandriati Primate Research Center, Bogor Agricultural University, Bogor Bimana Indomedical, Bogor
  • Uus Saepuloh Primate Research Center, Bogor Agricultural University, Bogor
  • Eric Hayes Porsolt SAS
  • Joko Pamungkas Primate Research Center, Bogor Agricultural University, Bogor Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor
  • Dondin Sajuthi Primate Research Center, Bogor Agricultural University, Bogor Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor
Keywords: Asthma, CCL7, gene characterization, MCP3, nonhuman primates

Abstract

Cynomolgus macaques (Macaca fascicularis) are an established animal model of asthma, which exhibit different responses to allergen exposure that are clinically relevant. The chemokine ligand gene (CCL7) encodes Monocyte Chemotactic Protein-3, which has an important role in asthma pathogenesis. While CCL7 polymorphism in humans is associated with asthma phenotype, very little is known about CCL7 in nonhuman primate models of respiratory disease. The objective of this study was to isolate and characterize CCL7 gene in cynomolgus macaques of Indonesian origin. In this study, we used sequencing and bioinformatics technique for gene isolation, characterization, and protein 3D structure prediction. We isolated a 2253 base-pair (bp) sequence of CCL7 in cynomolgus macaques, which exhibited 95% similarity in coding sequence to human CCL7. The amino acid sequence was more closely clustered with human CCL7 than with that of rodents. Importantly, the predictive protein structure of CCL7 was similar to that in humans. These similarities in CCL7 suggests the potential of cynomolgus macaque as a translational model to study asthma, particularly in the context of genetics and role of chemokines such as CCL7.

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Published
2019-12-02
Section
Short Communication