<p class="MsoNormal" style="text-align:justify;text-indent:14.15pt;"><strong><span style="font-size:9pt;">The excessive number of free radicals in human body could increase lipid peroxides and causes a variety of degenerative diseases. <em>Piper crocatum</em> is a plant containing natural chemicals that may have an antioxidant ability to inhibit fatty acid oxidation and reduce free radicals, however, there is no published scientific reports up to now. Therefore, the objective of this research is to study the ability of ethanol extract of <em>P. crocatum</em> as an antioxidant. The leaves of <em>P. crocatum</em> (25 g) were extracted with 100 ml of 70% ethanol. Antioxidant activity was measured by thiobarbituric acid method and compared with 2,2-diphenil-1-picryl hydrazyl method with α-tocopherol as the standard antioxidant. The results showed that the extract of 200 ppm inhibited fatty acids oxidation by 80.40% and IC</span></strong><strong><span style="font-size:5pt;">50</span></strong><strong><span style="font-size:9pt;"><span> </span>for free radical scavenging was 85.82 ppm. There was no significant different (α = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and α-tocopherol at 200 ppm. This study suggested that the leaves extract of <em>P. crocatum</em> has a potential natural antioxidant.</span></strong></p>

  • MUHAMMAD ALFARABI Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University
  • MARIA BINTANG Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University
  • . SURYANI Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University
  • MEGA SAFITHRI Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University
Keywords: <p class="MsoNormal" style="text-align, justify, text-indent, 14.15pt, "><strong><span style="font-size, 9pt, ">The excessive number of free radicals in human body could increase lipid peroxides and causes a variety of degenerative diseases. <em>Piper crocatum</em> is a plant containing natural chemicals that may have an antioxidant ability to inhibit fatty acid oxidation and reduce free radicals, however, there is no published scientific reports up to now. Therefore, the objective of this research is to study the ability of ethanol extract of <em>P. crocatum</em> as an antioxidant. The leaves of <em>P. crocatum</em> (25 g) were extracted with 100 ml of 70% ethanol. Antioxidant activity was measured by thiobarbituric acid method and compared with 2, 2-diphenil-1-picryl hydrazyl method with α-tocopherol as the standard antioxidant. The results showed that the extract of 200 ppm inhibited fatty acids oxidation by 80.40% and IC</span></strong><strong><span style="font-size, 5pt, ">50</span></strong><strong><span style="font-size, "><span> </span>for free radical scavenging was 85.82 ppm. There was no significant different (α = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and α-tocopherol at 200 ppm. This study suggested that the leaves extract of <em>P. crocatum</em> has a potential natural antioxidant.</span></strong></p>

Abstract

The excessive number of free radicals in human body could increase lipid peroxides and causes a variety of degenerative diseases. Piper crocatum is a plant containing natural chemicals that may have an antioxidant ability to inhibit fatty acid oxidation and reduce free radicals, however, there is no published scientific reports up to now. Therefore, the objective of this research is to study the ability of ethanol extract of P. crocatum as an antioxidant. The leaves of P. crocatum (25 g) were extracted with 100 ml of 70% ethanol. Antioxidant activity was measured by thiobarbituric acid method and compared with 2,2-diphenil-1-picryl hydrazyl method with α-tocopherol as the standard antioxidant. The results showed that the extract of 200 ppm inhibited fatty acids oxidation by 80.40% and IC50 for free radical scavenging was 85.82 ppm. There was no significant different (α = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and α-tocopherol at 200 ppm. This study suggested that the leaves extract of P. crocatum has a potential natural antioxidant.

Downloads

Download data is not yet available.
How to Cite
ALFARABIM., BINTANGM., SURYANI., & SAFITHRIM. (1). <p class="MsoNormal" style="text-align:justify;text-indent:14.15pt;"><strong><span style="font-size:9pt;">The excessive number of free radicals in human body could increase lipid peroxides and causes a variety of degenerative diseases. <em>Piper crocatum</em> is a plant containing natural chemicals that may have an antioxidant ability to inhibit fatty acid oxidation and reduce free radicals, however, there is no published scientific reports up to now. Therefore, the objective of this research is to study the ability of ethanol extract of <em>P. crocatum</em> as an antioxidant. The leaves of <em>P. crocatum</em> (25 g) were extracted with 100 ml of 70% ethanol. Antioxidant activity was measured by thiobarbituric acid method and compared with 2,2-diphenil-1-picryl hydrazyl method with α-tocopherol as the standard antioxidant. The results showed that the extract of 200 ppm inhibited fatty acids oxidation by 80.40% and IC</span></strong><strong><span style="font-size:5pt;">50</span></strong><strong><span style="font-size:9pt;"><span> </span>for free radical scavenging was 85.82 ppm. There was no significant different (α = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and α-tocopherol at 200 ppm. This study suggested that the leaves extract of <em>P. crocatum</em> has a potential natural antioxidant.</span></strong></p&gt;. HAYATI Journal of Biosciences, 17(4), 201. https://doi.org/10.4308/hjb.17.4.201
Section
Short Communication